Determination of Iron in Blood Serum by Spectrophotometric and Atomic Absorption Methods as a Comparative Study

Year 2013, Volume: 38 Issue: 3, 358 - 364, 01.09.2013

Usama Abu Mohsen Khaled İ Abu Elaish Mahmoud Issa

Abstract

Context: Iron is an essential element for most life on Earth, including human beings and its accurate determination is extremely important. A simple and sensitive spectrophotometric method has been used for the determination of iron concentration in blood serum and compare with atomic absorption spectrophotometric method. Objective: To find the appropriate, simple and accurate methods for iron determination in serum samples Design: A comparative study between two analytical methods. The methods are based on the reaction of iron with ferene reagent at acidic condition. The method of determining iron in serum by flame atomic absorption spectrophotometry with slurry nebulization into an air-acetylene to various flame has been used. Main Outcome Measure: Serum iron concentrations in different serum samples. Results: The results showed that distribution of iron content between serum samples are different, it was between 41 to 343.5 mcg/dL. Values of iron content measured in the serum samples with spectrophotometer method are in good agreement with the results obtained with atomic absorption method. Conclusions: The spectrophotometric method with ferene reagent can be successfully and accurately applied to the determination of iron in serum and it can be used instead atomic absorption.

Keywords

iron, serum, determination, ferene, spectrophotometry, atomic absorption

Kan Serumundaki Demir Miktarının Spektrofotometrik ve Atomik Absorpsiyon Metodlarıyla Karşılaştırmalı Olarak Tayini

Abstract

Amaç: Demir insanoğlu dahil dünyada ki birçok yaşam formu için gerekli bir element olup onun canlıdaki miktarının doğru tayini son derece önemlidir. Çalışmamızdaki amacımız kolay ve hassas olan spektrofotometrik metod ile kan serumundaki demir konsantrasyonlarını belirleyerek bulunan değerleri atomik absorpsiyon spektrofotometrik yöntemi ile karşılaştırılmaktadır. Materyal ve Metod: Serum örneklerinde demir tayini için kullanılabilecek en uygun, en basit ve en doğru yöntemi bulmak. İki analitik yöntem arasında karşılaştırmalı bir çalışma yapılması düşünüldü. Yöntemler asidik koşullarda ferene reaktifi ile demirin reaksiyonuna dayanır. Serum demir belirleme yöntemi olarak nebulizasyon ürününün bir hava-asetilen karışımı içerisinden geçirildiği alevli atomik absorpsiyon spektrofotometresi yöntemi ile yapıldı. Bulgular: Elde edilen bulgular; serum örnekleri arasındaki demir içeriği dağılımının 41 ile 343.5 mcg/dl aralığında farklılık gösterdiğini ortaya koymuştur. Serum örneklerinde spektrofotometre yöntemi ile ölçülen demir içeriğine ait değerler atomik absorpsiyon yöntemi ile belirlenen sonuçlarla uyum göstermektedir. Sonuç: Ferene reaktifinin kullanıldığı spektrofotometrik yöntem serum demir içeriğinin başarılı ve doğru olarak belirlenmesinde uygulanabilir ve ayrıca bu yöntem atomik absorpsiyon yöntemi yerine kullanılabilir.

Keywords

Demir, serum, belirleme, ferene, spektrofotometre, atomik absorpsiyon

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• Yazışma Adresi / Address for Correspondence: Dr. Usama Abu Mohsen Al Azhar University Faculty of Pharmacy Department of Pharmaceitical Chemistry Gaza-Palestine e-mail: [e-mail] geliş tarihi/received :09.10.2012 kabul tarihi/accepted:07.12.2012