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Tumor necrosis factor beta A329G gene polymorphism and the association between patients with acute myocardial infarction

Year 2013, Volume: 38 Issue: 3, 365 - 373, 01.09.2013

Abstract

Aim: In this study, we aim to evaluate the efficacy of the tumor necrosis factor-beta A329G gene polymorphism in patients admitted to emergency department with chest pain complaint and diagnosed acute myocardial infarction. Material and Method: This study was planned as a prospective, randomized, controlled study and we started the study after the approval of Cukurova University Ethics Committee. Blood samples were taken from the patients who gave permission. Analysis of blood samples were studied in the Biochemistry Laboratory of Mersin University. DNA was extracted by High Pure PCR Template Preparation kit (High Pure PCR Template Preparation kit, Roche Diagnostic, Germany). TNF-β gene polymorphism A329G mutations were determined by using the Light Cycler instrument detection kit (Roche diagnostic, GmbH, Mannheim, Germany) in Light Cycler Real Time PCR. Statistical analysis of data was performed with SPSS 11.5 software package. The statistical significance level of p

References

  • Lopez AD, Mathers CD, Ezzati M, Jamison DT, Murray CJ. Global and regional burden of disease and risk factors, 2001: systematic analysis of population health data. Lancet. 2006; 367:1747-57.
  • Türkiye’ de Sağlığa Bakış. TC Sağlık Bakanlığı. 2007; 47-50.
  • Libby P. Changing concepts of atherogenesis J Intern Med. 2000; 247: 349-358.
  • Kritchevsky SB, Cesari M, Pahor M. Inflammatory markers and cardiovascular health in older adults. Cardiovas Res. 2005; 66: 265–75.
  • Ross R. Atherosclerosis-an inflammatory disease. N Engl J Med. 1999; 340: 115-26.
  • Grundy SM. Atlas of aterosclerosis. Risk factors and treatment. 4th ed.1999. 1-30.
  • Güner İ, Özmen D, Bayındır O. Sitokinler. T Klin J Med Sci. 1997; 17: 65-74.
  • Messer G, Spengler U, Jung MC, Honold G, Blomer K, Pape GR, et al. Polymorphic structure of the tumor necrosis factor (TNF) locus: an NcoI polymorphism in the first intron of the human TNF-beta gene correlates with a variant amino acid in position 26 and a reduced level of TNF-beta production. J Exp Med. 1991; 173: 209-19.
  • Weinshenker BG, Hebrink D, Wingerchuk DM, Klein CJ, Atkinson E, O’Brien PC, et al. Genetic variants in the tumor necrosis factor receptor 1 gene in patients with MS. Neurology. 1999; 52: 1500-3.
  • Grünenfelder J, Umbehr M, Plass A, Bestmann L, Maly FE, Zünd G, et al. Genetic polymorphisms of apolipoprotein E4 and tumor necrosis factor ß as predisposing factors for increased inflammatory cytokines after cardiopulmonary bypass. J Thorac Cardiovasc Surg. 2004; 128: 92-97.
  • Liang WB, Lv ML, Su XW, Gao LB, Fang WL, Luo HB,et al. Association of tumor necrosis factor gene polymorphisms with susceptibility to dilated cardiomyopathy in a Han Chinese population. DNA Cell Biol. 2010; 29: 625-8.
  • Densem CG, Hutchinson IV, Yonan N, Brooks NH. Influence of tumor necrosis factor-alpha gene-308 polymorphism on the development of coronary vasculopathy after cardiac transplantation. Journal of Heart and Lung Transplantatıon. 2001; 20: 1265-73.
  • Kubota T, McNamara DM, Wang JJ, Trost M, McTiernan CF, Mann DL, et al. Effects of Tumor Necrosis Factor Gene Polymorphisms on Patients With Congestive Heart Failure. Circulation. 1998; 97: 2499–2501.
  • Shulz S, Schagdarsurengin U, Rehfeld D, Suss T, Werdan K, Muller-Werdan U, et al. Genetic impact of TNF-beta on risk factors for coronary atherosclerosis. European Cytokine Network. 2006; 17: 148-54.
  • Riha H, Hubacek JA, Poledne R, Kellovsky P, Brezina A, Pirk J. IL-10 and TNFβ gene polymorphisms have no major influence on lactate levels after cardiac surgery. European Journal of Cardio-Thoracic Surgery. 2006; 30: 54-8.
  • Iribarren JL, Sagasti FM, Jiménez JJ, Brouard M, Salido E, Martínez R,et al. TNFbeta+250 polymorphism and hyperdynamic state in cardiac surgery with extracorporeal circulation. Interact Cardiovasc Thorac Surg. 2008; 7: 1071-4.
  • Grundy SM. Posternak R, Greenland P, Smith S Jr, Fuster V. Assesment of cardiovasculer risk by use of multiple risk factor assesment equations. Circulation. 1999; 100: 1481-92.

Tümör nekroz faktör beta A329G gen polimorfizmi ve akut miyokard infarktüsü olan hastalar arasındaki ilişki

Year 2013, Volume: 38 Issue: 3, 365 - 373, 01.09.2013

Abstract

Amaç: Bu çalışmada; acil servise göğüs ağrısı şikayeti ile başvuran ve miyokard infarktüsü (MI) tanısı konulan hastalarda tümör nekroz faktör-beta (TNF-&#61538;) A329G gen polimorfizminin etkinliğinin araştırılması amaçlanmıştır. Gereç ve Yöntem: Prospektif, randomize, kontrollü olarak yapılması planlanan çalışmamıza Çukurova Üniversitesi Tıp Fakültesi Etik Kurulu&#8217;ndan onay alındıktan sonra başlandı. Çalışmaya onam veren hastalardan kan örneği alındı. Alınan kan örneklerinin analizi Mersin Üniversitesi Tıp Fakültesi Biyokimya Laboratuvarı&#8217;nda çalışıldı. DNA izolasyonu, DNA izolasyon kiti (High Pure PCR Template Preparation kit, Roche Diagnostic, Germany) ile yapıldı. TNF-&#946; A329G gen polimorfizmi Light Cycler mutasyon belirleme kiti (Roche diagnostic, GmbH, Mannheim, Germany) kullanılarak Light Cycler cihazında Real Time PCR ile saptandı. Verilerin istatistiksel analizi SPSS 11.5 paket programı ile yapıldı. Tüm testlerde istatistiksel önem düzeyi p < 0.05 olarak alındı. Bulgular: Bu çalışmaya miyokard infarktüsü tanısı konulan 90 hasta (78 erkek, 12 kadın) ve kontrol grubu olarak 78 sağlıklı birey (28 erkek, 50 kadın) dahil edildi. Tümör nekroz faktör-beta A329G polimorfizmi ile miyokard infarktüsü arasında istatistiksel olarak anlamlı bir ilişki bulunamadı. Miyokard infarktüs&#8217; lü hastaların % 42.2&#8217;sinde AG genotipi ve % 6.7&#8217;sinde ise GG genotipi saptandı. MI olan hastalarda kontrol grubuna göre LDL düzeyinin istatistiksel olarak anlamlı derecede yüksek olduğu (p = 0.021), HDL düzyinin ise istatistiksel olarak anlamlı derecede düşük olduğu (p = < 0.001) saptandı. Sonuç: TNF-&#946; A329G gen polimorfizmi ile Miyokard İnfarktüsü arasında bir ilişki olmadığı saptandı. Yüksek LDL ve düşük HDL düzeylerinin Mİ için risk faktörleri olduğu tespit edildi. Ülkemizde Türk toplumuna yönelik TNF-&#946; A329G gen polimorfizmi ile MI arasındaki ilişkiyi belirlemeye yönelik yapılan ilk çalışma olması açısından bu çalışma önemlidir. Bu çalışma, diğer çalışmalara ışık tutabilir ve tümör nekroz faktörün diğer polimorfizmleri araştırılarak muhtemel olası anlamlı bulgular elde edilebilir.

References

  • Lopez AD, Mathers CD, Ezzati M, Jamison DT, Murray CJ. Global and regional burden of disease and risk factors, 2001: systematic analysis of population health data. Lancet. 2006; 367:1747-57.
  • Türkiye’ de Sağlığa Bakış. TC Sağlık Bakanlığı. 2007; 47-50.
  • Libby P. Changing concepts of atherogenesis J Intern Med. 2000; 247: 349-358.
  • Kritchevsky SB, Cesari M, Pahor M. Inflammatory markers and cardiovascular health in older adults. Cardiovas Res. 2005; 66: 265–75.
  • Ross R. Atherosclerosis-an inflammatory disease. N Engl J Med. 1999; 340: 115-26.
  • Grundy SM. Atlas of aterosclerosis. Risk factors and treatment. 4th ed.1999. 1-30.
  • Güner İ, Özmen D, Bayındır O. Sitokinler. T Klin J Med Sci. 1997; 17: 65-74.
  • Messer G, Spengler U, Jung MC, Honold G, Blomer K, Pape GR, et al. Polymorphic structure of the tumor necrosis factor (TNF) locus: an NcoI polymorphism in the first intron of the human TNF-beta gene correlates with a variant amino acid in position 26 and a reduced level of TNF-beta production. J Exp Med. 1991; 173: 209-19.
  • Weinshenker BG, Hebrink D, Wingerchuk DM, Klein CJ, Atkinson E, O’Brien PC, et al. Genetic variants in the tumor necrosis factor receptor 1 gene in patients with MS. Neurology. 1999; 52: 1500-3.
  • Grünenfelder J, Umbehr M, Plass A, Bestmann L, Maly FE, Zünd G, et al. Genetic polymorphisms of apolipoprotein E4 and tumor necrosis factor ß as predisposing factors for increased inflammatory cytokines after cardiopulmonary bypass. J Thorac Cardiovasc Surg. 2004; 128: 92-97.
  • Liang WB, Lv ML, Su XW, Gao LB, Fang WL, Luo HB,et al. Association of tumor necrosis factor gene polymorphisms with susceptibility to dilated cardiomyopathy in a Han Chinese population. DNA Cell Biol. 2010; 29: 625-8.
  • Densem CG, Hutchinson IV, Yonan N, Brooks NH. Influence of tumor necrosis factor-alpha gene-308 polymorphism on the development of coronary vasculopathy after cardiac transplantation. Journal of Heart and Lung Transplantatıon. 2001; 20: 1265-73.
  • Kubota T, McNamara DM, Wang JJ, Trost M, McTiernan CF, Mann DL, et al. Effects of Tumor Necrosis Factor Gene Polymorphisms on Patients With Congestive Heart Failure. Circulation. 1998; 97: 2499–2501.
  • Shulz S, Schagdarsurengin U, Rehfeld D, Suss T, Werdan K, Muller-Werdan U, et al. Genetic impact of TNF-beta on risk factors for coronary atherosclerosis. European Cytokine Network. 2006; 17: 148-54.
  • Riha H, Hubacek JA, Poledne R, Kellovsky P, Brezina A, Pirk J. IL-10 and TNFβ gene polymorphisms have no major influence on lactate levels after cardiac surgery. European Journal of Cardio-Thoracic Surgery. 2006; 30: 54-8.
  • Iribarren JL, Sagasti FM, Jiménez JJ, Brouard M, Salido E, Martínez R,et al. TNFbeta+250 polymorphism and hyperdynamic state in cardiac surgery with extracorporeal circulation. Interact Cardiovasc Thorac Surg. 2008; 7: 1071-4.
  • Grundy SM. Posternak R, Greenland P, Smith S Jr, Fuster V. Assesment of cardiovasculer risk by use of multiple risk factor assesment equations. Circulation. 1999; 100: 1481-92.
There are 17 citations in total.

Details

Primary Language Turkish
Journal Section Research
Authors

Mehmet Canacankatan This is me

Ahmet Sebe This is me

Necmiye Canacankatan This is me

Mehmet Oguzhan Ay This is me

Meryem Genc Karanlık This is me

Ufuk Saracoglu This is me

Salim Satar This is me

Salim Satar This is me

Publication Date September 1, 2013
Published in Issue Year 2013 Volume: 38 Issue: 3

Cite

MLA Canacankatan, Mehmet et al. “Tümör Nekroz faktör Beta A329G Gen Polimorfizmi Ve Akut Miyokard infarktüsü Olan Hastalar arasındaki ilişki”. Cukurova Medical Journal, vol. 38, no. 3, 2013, pp. 365-73.