Effects of Magnetic Field on the Electrophysiological Parameters of Soleus Muscle in Streptozotocin-Induced Diabetic Rats

Year 2013, Volume: 38 Issue: 3, 426 - 433, 01.09.2013

Aykut Pelit Mustafa Emre Ayşe Demirkazık İsmail Günay

Abstract

Purpose: To investigate the effects of magnetic field (MF) on the resting membrane potential and action potential parameters of the Soleus muscle preparations in streptozotocin (STZ) induced diabetic rats. Methods: Forty male wistar rats were used in this study. The rats were divided into four groups; Group I (control), Group II (control with magnetic field), group III (diabetic), group IV (diabetic with magnetic field). The diabetes was induced by a single intravenous injection of STZ (45 mg/kg) in citrate buffer to the jugular vein under the ketamine and xylazine anesthetic combination. Resting membrane potential (RMP), muscle action potential (MAP), depolarization and half-repolarization time, MAP integral, depolarization and repolarization rates were recorded by means of the conventional microelectrode technique. Results: When group III and IV compared to group I, a significant decrease was found in the RMP (p

Keywords

Soleus muscle, streptozotocin, resting membrane potential, action potential, magnetic field

Streptozotosin ile Deneysel Diyabet Oluşturulan Sıçanların Soleus Kaslarının Elektrofizyolojik Parametrelerine Elektromanyetik Alanın Etkileri

Abstract

Amaç: Streptozotosin (STZ) ile deneysel diyabet oluşturulan sıçanlar elektromanyetik alana maruz bırakıldıktan sonra soleus kas preparatlarından dinlenim zar potansiyeli ve kas aksiyon potansiyelinin ölçülerek incelenmesi. Metod: Bu çalışmada 40 adet Wistar türü erkek sıçan kullanıldı. Sıçanlar dört gruba bölündü; Grup I (Kontrol), Grup II (Manyetik alan kontrolü), Grup III (Diyabetik), Grup IV (diyabet uygulanmış manyetik alan grubu). Diyabet tek doz halinde (45 mg/kg ) ketamin ve ksilazin kombinasyon anestezisi altında citrat tamponu içerisine karıştırılmış STZ olarak jügüler venden verildi. Konvensiyonel mikroeletrod tekniği ile dinlenim zar potansiyeli, aksiyon potansiyeli, depolarizasyon ve repolarizasyon süresi, kas aksiyon potansiyeli integrali kayıtlandı. Sonuçlar: Dinlenim zar potansiyeli, Grup I, III ve IV arasında anlamlı bir azalma bulunmuştur (p

Keywords

Soleus kası, streptozotosin, Dinlenim zar potansiyeli, Aksiyon Potansiyeli, Manyetik Alan.

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• Yazışma Adresi / Address for Correspondence: Dr. Aykut Pelit Çukurova University Medical Faculty Biophysics Department 01330 Balcali-ADANA e-mail: [e-mail]; [e-mail] geliş tarihi/received :13.10.2012 kabul tarihi/accepted:07.12.2012