The Relationship Between Insulin PSTI Polymorphism and Prostate Cancer in Turkish Population

Year 2013, Volume: 38 Issue: 3, 434 - 439, 01.09.2013

Muhammed Hamza Muslumanoglu Tanju Basmaci Selma Demir Ulusal Huseyin Aslan Emre Tepeli Muhsin Ozdemir Mehmet Turgut

Abstract

Purpose: There is positive correlation between insulin and various cancers such as colon, lung and endometrium. Protate cancer is the one of them. The aim of this study, to determine the incidance of INS+1127 PstI polymorphism, located in the 3" UTR of insulin gene, between prostate cancer cases and controls and to clarify if this polymorphism have any role in the etiology of prostate cancer. Methods: We investigated 71 prostate cancer cases and 119 normal controls unrelated to patients. INS+1127 PstI polymorphism was investigated with PCR and RFLP method. Results: At the end of study; INS+1127 PstI polymorphism genotypes out to be 67.6 % CC, 28.2 % CT, 4.2 % TT among prostatic cancer individuals and 78.2% CC, 21% CT, 0.8% TT among control group individuals. Conclusion: As a result, there was no significant difference between patients and control group, in the genotype distribution.

Keywords

INS, polymorphism, prostate cancer, PstI

Türk Popülasyonunda PstI Polimorfizminin Prostat Kanseri ile İlişkisinin Araştırılması

Abstract

Giriş: İnsülin ve kolon, akciğer ve endometrium kanserleri gibi bazı kanser türleri arasında pozitif bir korelasyon olduğu bilinmektedir. Prostat kanseri de bunlardan birisidir. Bu çalışmadaki amacımız insülin geni 3" UTR bölgesinde lokalize olan INS+1127 PstI polimosfizminin prostat kanseri etiyolojinde rolünü araştırmaktır. Materyal ve Metot: Çalışmaya 71 prostat kanseri tanısı almış hasta ve 119 normal kontrol bireyi dahil edilmiştir. INS+1127 PstI polimorfizm araştırması için PCR ve RFLP tekniği kullanılmıştır. Bulgular: Çalışmanın sonucunda INS geni PstI polimorfizmi genotipleri prostat kanserli hastalarda (n:71) %67,6 CC, %28,2 CT, %4,2 TT; kontrol grubunda (n: 119) %78,2 CC, %21 CT, %0,8 TT olarak belirlenmiştir. Sonuç: Hasta ve kontroller arasındaki genotip dağılımında istatistiksel olarak anlamlı bir farklılık bulunamamıştır (P=0,078).

Keywords

INS, polimorfizm, prostat kanseri, PstI

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• Yazışma Adresi / Address for Correspondence: Dr. Muhammed Hamza M üslümanoğlu Yildiz Technical University, Faculty of Science and Arts, Department of Molecular Biology, Davutpasa Street 124, 34210 Merter/Istanbul, e-mail: [e-mail] geliş tarihi/received :15.12.2012 kabul tarihi/accepted:28.12.2012