The levels of oxidative stress biomarkers of liver and mammary tissues of apparently mice.

Year 2013, Volume: 38 Issue: 1, 7 - 14, 01.03.2013

Ergul Belge Kurutaş Figen Doran Yakup Gümüşalan Yalçın Atlı

Abstract

Objective: In the present study, the levels of antioxidant systems such as glucose-6-phosphate dehydrogenase (G6PDH), catalase (CAT), superoxide dismutase (SOD), glutathione-S-transferase (GST) and, also reduced glutathione (GSH) in liver and breast tissues were examined in order to obtain basal data for subsequent toxicological investigations. Additionally, the level of thiobarbituric acid reactive substance (TBARS) was measured in liver and breast tissues as an index of lipid peroxidation. Materials and Methods: Sixty-six apparently healthy mice (36 male, 30 female) had normal liver and breast tissues (histopathological data) were taken into the study. The tissues were homogenised with ice-cold 1.15 % KCI. The activities of antioxidant enzymes, the levels of GSH and TBARS were measured as spectrophotometric in the supernatant obtained from centrifugation at 14.000 rpm. Results: The levels of antioxidant systems and TBARS in liver were significantly higher than those found in breast tissue of female and male mice (P < 0.05). However, the levels of CAT, SOD, GST, GSH and TBARS in breast tissue except G6PDH enzyme were not significantly different between female and male mice (P > 0.05). On the other hand, the levels of CAT, SOD, GST, GSH and TBARS in male liver except G6PDH enzyme were higher than female liver (P < 0.05). Conclusion: Results suggest that antioxidant defense of the liver tissue was higher compared to breast tissue possibly due to a compensatory response to various toxic substances in liver and thereby protects the cells against oxidative damage.

Keywords

Antioxidant systems, TBARS, liver, breast

Sağlıklı görünen farelerin karaciğer ve meme dokularında oksidatif stres biyobelirteçlerinin düzeyleri

Abstract

Giriş: Bu çalışmada ileride yapılacak toksikolojik araştırmalara temel veri sağlamak amacıyla karaciğer ve meme dokularında antioksidan sistemler [glukoz-6-fosfat dehidrogenaz (G6PDH), katalaz (CAT), süperoksit dismutaz (SOD), glutatyon-S-transferaz (GST), redükte glutatyon (GSH)] incelendi. Buna ek olarak, lipit peroksidasyonun bir göstergesi olarak karaciğer ve meme dokularında tiyobarbiturik asit reaktif maddesi (TBARS) ölçüldü. Materyal ve Metod: Sağlıklı görünen, histopatolojik inceleme sonucu normal karaciğer ve meme dokusu bulgusu bulunan 66 fare (36 erkek, 30 dişi) çalışmaya alındı. Dokular soğuk % 1,15 KCI ile homojenize edildi. Antioksidan enzimler, GSH ve TBARS düzeyi 14.000 rpm"de santrifüjden sonra elde edilen süpernatant"ta spektrofotometrik olarak ölçüldü. Bulgular: Erkek ve dişi farelerin karaciğer dokularındaki antioksidan sistemlerin ve TBARS düzeylerinin meme dokusundan yüksek olduğu gözlendi (P < 0,05). Bununla birlikte, meme dokusundaki G6PDH hariç CAT, SOD, GST, GSH ve TBARS düzeylerinin erkek ve dişi fareler arasında farklılık göstermediği saptandı (P > 0,05). Öte yandan, erkek fare karaciğerinde G6PDH hariç CAT, SOD, GST, GSH ve TBARS düzeylerinin dişi karaciğerine göre daha yüksek bulundu (P < 0,05). Sonuç: Sonuçlar, karaciğer dokusunda antioksidan savunmanın meme dokusuna oranla yüksek olmasının, karaciğerde olası çeşitli toksik maddelere karşı kompensatuar yanıta bağlı olabileceğini ve bu şekilde hücreleri oksidatif hasara karşı koruyabileceğini göstermiştir.

Keywords

Antioksidan sistemler, TBARS, karaciğer, meme

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• Yazışma Adresi / Address for Correspondence: Dr.Ergül Belge Kurutaş Kahramanmaraş Sütçü İmam Üniversitesi Biyokimya Anabilim Dalı Yörük Selim mah., Hastane cad., No: 32 46050/ KAHRAMANMARAŞ Tel: [phone] Faks: 0344 2212371 geliş tarihi/received :26.07.2012 kabul tarihi/accepted:21.08.2012