BibTex RIS Cite

An Investigation of Correlation between Electrophysiological and Functional Recovery after the Sciatic Nerve Injury

Year 2012, Volume: 37 Issue: 4, 177 - 185, 01.12.2012

Abstract

Purpose: Video or photo assisted footprint analysis method is used to determine the motor and sensorial development instead of classic walking track footprint analysis in experimental peripheral nerve injury. Besides, the sucrose-gap method is used for measuring the electrophysiological activity in the sciatic nerves in-vitro. The aim of this study is to investigate the relationship between functional and electrophysiological recovery during the nerve regeneration in Wistar rats. Methods: In the experiments, after the unilateral sciatic nerve crushing, the rats were evaluated at the preoperative and 2nd, 4th, 6th and 8th weeks postoperative using the sucrose gap method, and photo assisted footprint method. The compound action potentials (CAP), the Peak- time (PT) and the ½ Falling- time (1/2FT) were measured, and compared to functional results. Results: Two weeks after being crushed sciatic nerves, complete function loss was seen operated legs in all rats. The amplitude of CAP was determined too small. The PT and the 1/2FT values were three fold longer than intact. However, following 4th - 8th weeks, the amplitude of CAP and other parameters of CAP were closed to intact values. Conclusion: The findings indicated that the results of the functional recovery were correlated to electrophysiological results. However, functional results showed almost full functional recovery in the 4th week, the electrophysiological results did not reach to intact values in the 8th week. We conclude that photo assisted footprint analysis method and sucrose-gap technique, which are useful functional and electrophysiological methods to produce complementary knowledge with each other in the investigation of experimental peripheral nerve regeneration.

References

  • Ide C. Peripheral nerve regeneration. Neuroscience Research. 1996; 25:101–21.
  • Stoll G, Muller HW. Nerve injury, axonal degeneration and neural regeneration: Basic insights, Brain Pathology. 1999; 9:313-25.
  • Sunderland S. Nerves and nerve injuries, 2nd Ed., New York: Churchill Livingstone. 1978.
  • Black JA, Kocsis JD, Waxman SG. Ion channel organization of myelinated fiber. Trends in Neurosciences. 1990; 13:48-54.
  • Bowe C, Hildebrand MC, Kocsis JD, Waxman SG. Morphological and physiological properties of neurons after long-term axonal regeneration: observations on chronic and delayed sequelae of peripheral nerve injury. J. Neurol. Sci. 1989; 91:25992.
  • Hildebrand C, Bowe CM, Remahl IN. Myelination and myelin sheath remodelling in normal and pathological PNS nerve fibres. Prog. Neurobiol. 1994; 43:85-141.
  • Ritchie JM. Sodium and potassium channels in regenerating and developing mammalian myelinated nerves, Proc. R. Soc. Lond. B. Biol. Sci. 1982; 215:273-87.
  • Waxman SG. Acquired channelopathies in nerve injury and MS. Neurology. 2001; 56:1621-7.
  • De Medinaceli L, Freed WJ, Wyatt RJ. An index of the functional condition of rat sciatic nerve based on measurements made from walking tracks. Exp Neurol. 1982; 77: 634–43.
  • Bervar M. Video analysis of standing-an alternative footprint analysis to assess functional loss following injury to the rat sciatic nerve. J. Neurosci. Methods. 2000; 102:109-16.
  • Varejao AS, Meek MF, Ferreira AJ, Patricio JA, Cabrita AM. Functional evaluation of peripheral nerve regeneration in the rat: walking track analysis. J. Neurosci. Methods. 2001; 108:1-9.
  • Lowden IMR, Seaber AV, Urbaniak JR. An improved method of recording rat tracks for measurement of the sciatic functional index of deMedinaceli. J Neurosci Methods. 1988; 24:279–81.
  • Walker JL, Resig P, Sisken BF, Guarnieri S, Evans JM. Improved footprint analysis using video recording to assess functional recovery following injury to the rat sciatic nerve. Rest Neurol Neurosci. 1994; 6:189– 93.
  • Stampfli R. A new method for measuring membrane potentials with external electrodes. Experientia. 1954; 10: 508-9.
  • Gordon TR, Kocsis JD, Waxman SG. TEA-sensitive potassium channels and inward rectification in regenerated rat sciatic nerve. Muscle and Nerve. 1991; 14:640-46.
  • Nonaka T, Honmou O, Sakai J, Hashi K, Kocsis JD. Excitability changes of dorsal root axons following nerve injury: implications for injury-induced changes in axonal Na(+) channels. Brain Res. 2000; 859:2805.
  • Sakai J, Honmou O, Kocsis JD, Hashi K. The delayed depolarization in rat cutaneous afferent axons is reduced following nerve transection and ligation, but not crush: implications for injury-induced axonal Na+ channel reorganization. Muscle Nerve. 1998; 21: 1040-7.
  • Guven M, Mert T, Gunay I. Ag/AgCl Electrodes and Low-Cost Chloriding System. C.U. Journal of Health Sciences. 2003; 17:1-6.
  • Güven M, Mert T, Gunay I. Effect of tramadol on nerve action potentials. Comparations with lidocaine and benzocaine. Int. J Neurosci. 2005; 115(3): 35565.
  • Mert T, Gunes Y, Guven M, Gunay I, Ozcengiz D. Comparison of nerve conduction blocks by an opioid and a local anesthetic. Eur. J. Pharmacol. 2002; 439:77-81.
  • Rasband MN, Shrager P. Ion channel sequestration in central nervous system axons. J Physiol. 2000; 15:63-73

Siyatik Sinir Ezilmesinden Sonra Elektrofizyolojik ve Fonksiyonel İyileşme İlişkisinin Araştırılması

Year 2012, Volume: 37 Issue: 4, 177 - 185, 01.12.2012

Abstract

Amaç: Deneysel periferik sinir yaralanmalarında motor ve duyusal iyileşmeyi belirlemek için klasik yürüme izi analiz yöntemi yerine, video ya da fotoğraf destekli footprint analiz yöntemi kullanılabilmektedir. Bunun yanında in-vitro bir yöntem olan sukroz-gap yöntemi de sıçan siyatik sinirlerinde elektrofizyolojik aktivitenin ölçülmesinde kullanılmaktadır. Bu çalışmadaki amacımız Wistar türü sıçanlarda, sinir rejenerasyonu sırasındaki fonksiyonel ve elektrofizyolojik iyileşme ilişkisini araştırmaktır. Yöntem: Çalışmalarımızda, siyatik sinirlerde tek taraflı ezildikten sonra, sıçanlar operasyon öncesi, 2., 4., 6. ve 8. haftalarda sukroz-gap ve fotoğraf destekli footprint yöntemi ile değerlendirildi. Bileşik aksiyon potansiyelleri (CAP), yükselme süresi (PT) ve ½ düşme süresi ölçüldü (1/2 FT) ve fonksiyonel sonuçlarla karşılaştırıldı. Bulgular: Siyatik sinirlerin ezilmesinden iki hafta sonra tüm sıçanların ezilmiş sinirin bulunduğu ayaklarda tam fonksiyon kaybı oldu. CAP genliği çok düşük olarak belirlendi. PT ve 1/2FT değerleri normal değerlerinden üç kere daha uzundu. Buna rağmen, takip eden 4. - 8. haftalarda, CAP genliği ve diğer CAP parametreleri normale yakın değerlere geri döndü. Sonuç: Bulgular, fotoğraf destekli footprint yöntemi sonuçları ile, elektrofizyolojik sonuçların yakın bağlantı içinde olduğuna işaret etmektedir. Bununla birlikte, fonksiyonel sonuçlar, 4. haftada neredeyse tam iyileşmeyi gösterirken, elektrofizyolojik ölçümler 8. haftaya kadar normal değerlere ulaşmadı. Sonuç olarak, fotoğraf destekli footprint analiz ve sukroz-gap yönteminin deneysel periferik sinir rejenerasyonun araştırılmasında birbirini tamamlayıcı bilgiler ürettiğini düşünüyoruz.

References

  • Ide C. Peripheral nerve regeneration. Neuroscience Research. 1996; 25:101–21.
  • Stoll G, Muller HW. Nerve injury, axonal degeneration and neural regeneration: Basic insights, Brain Pathology. 1999; 9:313-25.
  • Sunderland S. Nerves and nerve injuries, 2nd Ed., New York: Churchill Livingstone. 1978.
  • Black JA, Kocsis JD, Waxman SG. Ion channel organization of myelinated fiber. Trends in Neurosciences. 1990; 13:48-54.
  • Bowe C, Hildebrand MC, Kocsis JD, Waxman SG. Morphological and physiological properties of neurons after long-term axonal regeneration: observations on chronic and delayed sequelae of peripheral nerve injury. J. Neurol. Sci. 1989; 91:25992.
  • Hildebrand C, Bowe CM, Remahl IN. Myelination and myelin sheath remodelling in normal and pathological PNS nerve fibres. Prog. Neurobiol. 1994; 43:85-141.
  • Ritchie JM. Sodium and potassium channels in regenerating and developing mammalian myelinated nerves, Proc. R. Soc. Lond. B. Biol. Sci. 1982; 215:273-87.
  • Waxman SG. Acquired channelopathies in nerve injury and MS. Neurology. 2001; 56:1621-7.
  • De Medinaceli L, Freed WJ, Wyatt RJ. An index of the functional condition of rat sciatic nerve based on measurements made from walking tracks. Exp Neurol. 1982; 77: 634–43.
  • Bervar M. Video analysis of standing-an alternative footprint analysis to assess functional loss following injury to the rat sciatic nerve. J. Neurosci. Methods. 2000; 102:109-16.
  • Varejao AS, Meek MF, Ferreira AJ, Patricio JA, Cabrita AM. Functional evaluation of peripheral nerve regeneration in the rat: walking track analysis. J. Neurosci. Methods. 2001; 108:1-9.
  • Lowden IMR, Seaber AV, Urbaniak JR. An improved method of recording rat tracks for measurement of the sciatic functional index of deMedinaceli. J Neurosci Methods. 1988; 24:279–81.
  • Walker JL, Resig P, Sisken BF, Guarnieri S, Evans JM. Improved footprint analysis using video recording to assess functional recovery following injury to the rat sciatic nerve. Rest Neurol Neurosci. 1994; 6:189– 93.
  • Stampfli R. A new method for measuring membrane potentials with external electrodes. Experientia. 1954; 10: 508-9.
  • Gordon TR, Kocsis JD, Waxman SG. TEA-sensitive potassium channels and inward rectification in regenerated rat sciatic nerve. Muscle and Nerve. 1991; 14:640-46.
  • Nonaka T, Honmou O, Sakai J, Hashi K, Kocsis JD. Excitability changes of dorsal root axons following nerve injury: implications for injury-induced changes in axonal Na(+) channels. Brain Res. 2000; 859:2805.
  • Sakai J, Honmou O, Kocsis JD, Hashi K. The delayed depolarization in rat cutaneous afferent axons is reduced following nerve transection and ligation, but not crush: implications for injury-induced axonal Na+ channel reorganization. Muscle Nerve. 1998; 21: 1040-7.
  • Guven M, Mert T, Gunay I. Ag/AgCl Electrodes and Low-Cost Chloriding System. C.U. Journal of Health Sciences. 2003; 17:1-6.
  • Güven M, Mert T, Gunay I. Effect of tramadol on nerve action potentials. Comparations with lidocaine and benzocaine. Int. J Neurosci. 2005; 115(3): 35565.
  • Mert T, Gunes Y, Guven M, Gunay I, Ozcengiz D. Comparison of nerve conduction blocks by an opioid and a local anesthetic. Eur. J. Pharmacol. 2002; 439:77-81.
  • Rasband MN, Shrager P. Ion channel sequestration in central nervous system axons. J Physiol. 2000; 15:63-73
There are 21 citations in total.

Details

Primary Language Turkish
Journal Section Research
Authors

Mustafa Güven This is me

İbrahim Kahraman This is me

İsmail Günay This is me

Publication Date December 1, 2012
Published in Issue Year 2012 Volume: 37 Issue: 4

Cite

MLA Güven, Mustafa et al. “Siyatik Sinir Ezilmesinden Sonra Elektrofizyolojik Ve Fonksiyonel İyileşme İlişkisinin Araştırılması”. Cukurova Medical Journal, vol. 37, no. 4, 2012, pp. 177-85.