Sıçanlarda immünsupresyonsuz xenotransplantasyon uygulamasının etkililiği

Year 2019, Volume: 44 Issue: 3, 782 - 787, 30.09.2019

Ebru Kanımdan Emrah Yücesan Beyza Göncü Burcu Özdemir Oğuz İdiz Yeliz Emine Ersoy Fahri Akbaş Erhan Ayşan

https://doi.org/10.17826/cumj.486224

Abstract

Amaç: Çalışmamızın amacı, insan paratiroid dokularından izole edilen hiperplazik hücrelerin, sıçanlara immünsupresan verilmeden transplante edilerek transplantasyon verimliliğinin incelenmesidir. 

Gereç ve Yöntem: Çalışmada, paratiroid hiperplazi tanısıyla opere edilen 62 yaşında erkek hastadan izole edilen hiperplazik paratiroid hücreleri kullanıldı. Alıcı olarak üç adet erkek Sprague Dawley sıçan kullanıldı. İnsandan elde edilen hücreler sıçanlara immünsupresan uygulanmadan transplante edildi. Transplantasyon işlemi sırasında hücreler doğrudan sırasıyla, vena portaya, vena cavaya ve periferik vene enjekte edildi. Transplantasyon başarısının takibi açısından total parathormon (PTH) ve serum kalsiyum düzeyleri, transplantasyon öncesinde ve sonrasında ölçüldü.

Bulgular: Sıçanların transplantasyon öncesi total PTH  düzeyleri 1,4-4,2 pg/mL aralığında, 45. gün değerleri ise 6,2-17,1 pg/mL aralığında tespit edildi. 

Sonuç: Periferik dolaşımdan ve vena portadan yapılan transplantasyonun, vena cavaya göre daha başarılı olduğu belirlendi. Sonuç olarak nakil işleminde periferik venden hücre naklinin yararlı olabileceği düşünüldü. Sunduğumuz çalışmanın ilerleyen dönemlerde hayvan sayısı arttırılarak tekrarlanması araştırmayı kuvvetlendirecektir.

Keywords

Paratiroit hiperplazisi, parathormon, zenotransplantasyon

Efficacy of xenotransplantation without immunosuppression in rats

Abstract

Purpose: .The aim of this study was to transplant hyperplastic cells obtained from human parathyroid tissues into the rats without immunsupression, and also evaluate the efficiency. 

Materials and Methods: Present study,hyperplasic parathyroid tissues were obtained and cells were isolated from a 62-year-old male.  Three male rats were used as recipients.Cells were obtained from human,transplanted into different anatomical locations of rats without immunosuppression. During the transplantation, cells were injected directly into the vena porta, vena cava and peripheral vena.Total parathormone (PTH) and serum calcium  levels were measured before and after transplantation in terms of follow-up of transplantation success.

Results: Total PTH levels of rats, before transplantation were determined 1.4-4.2 pg/mL and throughout 45 days values were detected 6.2-17.1 pg/mL. 

Conclusion: Peripheral circulation and vena porta transplantation was found more successful than vena cava.As a result, cell transplantation from the peripheral vein might be useful technique.In the future, new studies should be carried out by increasing the number of animals will be strengthen this research. 

Keywords

Parathyroid hyperplasia, parathormone, xenotransplantation

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