Effect of Ferula elaeochytris on irisin levels in rat heart

Year 2021, Volume: 46 Issue: 2, 473 - 480, 30.06.2021

Nadire Eser Ahmet Türk Abdullah Karadağ Atila Yoldaş

https://doi.org/10.17826/cumj.840681

Cited By: 1

Abstract

Purpose: The aim of this study was to determine the levels of irisin in the heart, which is a vital organ, in rats of different ages and to reveal whether Ferula elaeochytris (FE) has an effect on the expression of the irisin.
Materials and Methods: Our study was designed in six groups, young (4 months), middle age (12 months), old (24 months) rats which were given FE extract for 8 weeks and their control groups. After that their heart tissues were taken and irisin levels were measured by immunohistochemical staining method.
Results: The obtained results were analyzed by performing histoscoring. It was observed that the level of irisin in old rats was statistically significantly lower in young and middle age rats. In addition, irisin level of in all groups administered with FE extract was found significantly increased.
Conclusion: FE extract increases the level of irisin through other pathways besides its protective effect against tissue damage.

Keywords

Irisin, Ferula elaeochytris, ageing, heart, rat

Supporting Institution

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Project Number

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Ferula elaeochytrisin sıçan kalbinde irisin düzeylerine etkisi

Abstract

Amaç: Bu çalışmanın amacı, farklı yaşlardaki sıçanlarda hayati bir organ olan kalpte bulunan irisin düzeylerini belirlemek ve Ferula elaeochytris'in (FE) irisin ekspresyonu üzerine etkisi olup olmadığını ortaya çıkarmaktır..
Gereç ve Yöntem: Çalışmamız 8 hafta boyunca FE ekstraktı verilmiş genç (4 ay), orta yaş (12 ay), yaşlı (24 ay) sıçanlar ve onların kontrol grupları olmak üzere altı grup şeklinde düzenlenmiştir. Ardından kalp dokuları alınarak, kalp dokusunda irisin seviyeleri immünohistokimyasal boyama yöntemi ile ölçülmüştür.
Bulgular: Elde edilen sonuçlar histoskorlama yapılarak analiz edilmiştir. Yaşlı sıçanlarda irisin seviyesinin genç ve orta yaş sıçanlara göre istatistiksel olarak anlamlı derecede düşük olduğu gözlenmiştir. Ayrıca FE ekstraktı verilmiş bütün gruplarda irisin düzeylerinde istatistiksel olarak anlamlı derecede anlamlı artış olduğu bulunmuştur.
Sonuç: FE ekstraktının doku hasarına karşı koruyucu etkisinin dışında başka yolaklar aracılığıyla da irisin seviyesini artırdığı düşünülmektedir.

Keywords

İrisin, Ferula elaeochytris, yaşlanma, kalp, sıçan

Project Number

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References

• 1. Boström P, Wu J, Jedrychowski MP, et al. A PGC1-α-dependent myokine that drives brown-fat-like development of white fat and thermogenesis. Nature 2012;481(7382):463-8 doi: 10.1038/nature10777[published Online First: Epub Date]|.
• 2. Aypak Aİ, Ünübol S. İrisin ve Metabolik Etkileri. 2016;11(1):15-21
• 3. Hecksteden A, Wegmann M, Steffen A, et al. Irisin and exercise training in humans - results from a randomized controlled training trial. BMC medicine 2013;11:235 doi: 10.1186/1741-7015-11-235[published Online First: Epub Date]|.
• 4. Hofmann T, Elbelt U, Ahnis A, Kobelt P, Rose M, Stengel A. Irisin Levels are Not Affected by Physical Activity in Patients with Anorexia Nervosa. Frontiers in endocrinology 2014;4:202 doi: 10.3389/fendo.2013.00202[published Online First: Epub Date]|.
• 5. Timmons JA, Baar K, Davidsen PK, Atherton PJ. Is irisin a human exercise gene? Nature 2012;488(7413):E9-10; discussion E10-1 doi: 10.1038/nature11364[published Online First: Epub Date]|.
• 6. Ghanbari-Niaki A, Saeidi A, Ahmadian M, et al. The combination of exercise training and Zataria multiflora supplementation increase serum irisin levels in postmenopausal women. Integr Med Res 2018;7(1):44-52 doi: 10.1016/j.imr.2018.01.007[published Online First: Epub Date]|.
• 7. Altundag E, Ozturk M. Ethnomedicinal studies on the plant resources of east Anatolia, Turkey. Procedia - Social and Behavioral Sciences 2011;19:756-77 doi: https://doi.org/10.1016/j.sbspro.2011.05.195[published Online First: Epub Date]|.
• 8. Güzel Y, Güzelşemme M, Miski M. Ethnobotany of medicinal plants used in Antakya: A multicultural district in Hatay Province of Turkey. Journal of ethnopharmacology 2015;174:118-52 doi: 10.1016/j.jep.2015.07.042[published Online First: Epub Date]|.
• 9. Eser N, Yoldas A. Identification of heat-resistant chemical components of Ferula elaeochytris root extracts by gas chromatographymass spectrometry. Tropical Journal of Pharmaceutical Research 2019;18:55 doi: 10.4314/tjpr.v18i1.9[published Online First: Epub Date]|.
• 10. Eser N, Yoldaş A, Yigin A, et al. The protective effect of Ferula elaeochytris on age-related erectile dysfunction. Journal of ethnopharmacology 2020;258:112921 doi: https://doi.org/10.1016/j.jep.2020.112921[published Online First: Epub Date]|.
• 11. Uyar A, Yaman T, Keles OF, et al. Reduction of hepatorenal and pancreatic damage by Ferula elaeochytris extract in STZ induced diabetic rats. Biotechnic & histochemistry : official publication of the Biological Stain Commission 2020:1-13 doi: 10.1080/10520295.2020.1753239[published Online First: Epub Date]|.
• 12. Labat-Robert J, Robert L. Longevity and aging. Mechanisms and perspectives. Pathologie-biologie 2015;63(6):272-6 doi: 10.1016/j.patbio.2015.08.001[published Online First: Epub Date]|.
• 13. Bimakr M, Rahman RA, Taip FS, et al. Comparison of different extraction methods for the extraction of major bioactive flavonoid compounds from spearmint (Mentha spicata L.) leaves. Food and bioproducts processing : transactions of the Institution of Chemical Engineers, Part C. 2011;89(1):67-72 doi: 10.1016/j.fbp.2010.03.002[published Online First: Epub Date]|.
• 14. Hsu SM, Raine L, Fanger H. Use of avidin-biotin-peroxidase complex (ABC) in immunoperoxidase techniques: a comparison between ABC and unlabeled antibody (PAP) procedures. The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society 1981;29(4):577-80 doi: 10.1177/29.4.6166661[published Online First: Epub Date]|.
• 15. Kuloglu T, Aydin S. Immunohistochemical expressions of adropin and ınducible nitric oxide synthase in renal tissues of rats with streptozotocin-ınduced experimental diabetes. Biotechnic & histochemistry : official publication of the Biological Stain Commission 2014;89(2):104-10 doi: 10.3109/10520295.2013.821713[published Online First: Epub Date]|.
• 16. Harms M, Seale P. Brown and beige fat: development, function and therapeutic potential. Nature medicine 2013;19(10):1252-63 doi: 10.1038/nm.3361[published Online First: Epub Date]|.
• 17. Kenyon CJ. The genetics of ageing. Nature 2010;464(7288):504-12 doi: 10.1038/nature08980[published Online First: Epub Date]|.
• 18. Rana KS, Arif M, Hill EJ, et al. Plasma irisin levels predict telomere length in healthy adults. AGE 2014;36(2):995-1001 doi: 10.1007/s11357-014-9620-9[published Online First: Epub Date]|.
• 19. Belviranlı M, Okudan N. Exercise training increases cardiac, hepatic and circulating levels of brain-derived neurotrophic factor and irisin in young and aged rats. Hormone molecular biology and clinical investigation 2018;36(3) doi: 10.1515/hmbci-2018-0053[published Online First: Epub Date]|.
• 20. Huh JY, Mougios V, Kabasakalis A, et al. Exercise-induced irisin secretion is independent of age or fitness level and increased irisin may directly modulate muscle metabolism through AMPK activation. The Journal of clinical endocrinology and metabolism 2014;99(11):E2154-61 doi: 10.1210/jc.2014-1437[published Online First: Epub Date]|.
• 21. Emanuele E, Minoretti P, Pareja-Galeano H, Sanchis-Gomar F, Garatachea N, Lucia A. Serum irisin levels, precocious myocardial infarction, and healthy exceptional longevity. The American journal of medicine 2014;127(9):888-90 doi: 10.1016/j.amjmed.2014.04.025[published Online First: Epub Date]|.
• 22. Aydin S, Kuloglu T, Aydin S, et al. Cardiac, skeletal muscle and serum irisin responses to with or without water exercise in young and old male rats: cardiac muscle produces more irisin than skeletal muscle. Peptides 2014;52:68-73 doi: 10.1016/j.peptides.2013.11.024[published Online First: Epub Date]|.
• 23. Xie C, Zhang Y, Tran TD, et al. Irisin Controls Growth, Intracellular Ca2+ Signals, and Mitochondrial Thermogenesis in Cardiomyoblasts. PloS one 2015;10(8):e0136816 doi: 10.1371/journal.pone.0136816[published Online First: Epub Date]|.
• 24. Kuloglu T, Aydin S, Eren MN, et al. Irisin: a potentially candidate marker for myocardial infarction. Peptides 2014;55:85-91 doi: 10.1016/j.peptides.2014.02.008[published Online First: Epub Date]|.
• 25. Alkhatib R, Hennebelle T, Joha S, et al. Activity of elaeochytrin A from Ferula elaeochytris on leukemia cell lines. Phytochemistry 2008;69(17):2979-83 doi: 10.1016/j.phytochem.2008.09.019[published Online First: Epub Date]|.
• 26. Assyov Y, Gateva A, Karamfilova V, et al. Impact of testosterone treatment on circulating irisin in men with late-onset hypogonadism and metabolic syndrome. The Aging Male 2020:1-7 doi: 10.1080/13685538.2020.1770721[published Online First: Epub Date]|.
• 27. Zhang X, Hu C, Kong C-Y, et al. FNDC5 alleviates oxidative stress and cardiomyocyte apoptosis in doxorubicin-induced cardiotoxicity via activating AKT. Cell Death & Differentiation 2020;27(2):540-55 doi: 10.1038/s41418-019-0372-z[published Online First: Epub Date]|.