Siklofosfamide maruz kalmış sıçanların dilaltı bezi üzerine kurkuminin etkisi

Year 2021, Volume: 46 Issue: 3, 897 - 903, 30.09.2021

Ahmad Yahyazadeh

Abstract

Amaç: Bu çalışma, siklofosfamidin (CY) 12 haftalık sıçanların dil altı bezleri üzerindeki etkisini ve ayrıca kurkuminin (CR) bu dokulardaki olası morfometrik değişimi üzerindeki olası etkinliğini araştırdı.
Gereç ve Yöntem: Otuz beş yetişkin erkek Wistar albino sıçan rastgele seçilerek aşağıdaki gibi yedi sıçandan oluşan beş gruba ayrıldı: kontrol (Con), zeytin (OV), CY, CR ve CY + CR. Dil Dil altı bez yapılarının ortalama hacimleri ve müköz ve seröz hücre sayısı stereolojik yöntemi kullanılarak tahmin edildi.
Bulgular: CY grubundaki mükös hücre ve seröz hücre sayısı, Con grubuna göre anlamlı olarak azaldığını bulduk. Ayrıca CY grubundaki mükös asinüsler, seröz asinüsler ve interkalat kanalların toplam hacmileri ile mükös asinüslerin stromaya hacim fraksiyon oranı, Con grubuna göre anlamlı olarak azalmıştı. Buna ek olarak, Con grubuna kıyasla CY grubunda toplam stroma hacminde anlamlı ölçüde bir artış gözlemlendi. CY+CR grubundaki müköz hücre ve seröz hücre sayısı, Con grubuna göre anlamlı olarak artmıştır.
Sonuç: CY tedavisinin dil altı bezleri üzerinde zararlı bir etkiye neden olduğunu ve CR uygulamasının da CY'nin neden olduğu değişiklikleri iyileştirdiğini düşündük.

Keywords

Dilaltı bezi, kurkumin, rat, siklofosfamid

Effect of curcumin on rat sublingual gland exposed to cyclophosphamide

Abstract

Purpose: This study investigated the effect of cyclophosphamide (CY) on the sublingual glands of 12 weeks old rats, as well as possible efficacy of curcumin (CR) on morphometrical change in these tissues.
Materials and Methods: Thirty-five adult male Wistar albino rats were randomly selected and divided into five group of seven rats as: control (Con), olive (OV), CY, CR, and CY+CR. The mean volumes of sublingual gland structures and the number of mucous and serous cells were estimated using stereological method.
Results: We found that the number of mucous and serous cells was significantly decreased in the CY group than the Con group. The total volume of mucous acini, serous acini, and intercalated ducts, as well volume fraction ratio of mucous acini to stroma was significantly decreased in the CY group than the Con group. By the contrary, there was a significant increase in the total volume of stroma in the CY group when compared with the Con group). In the CY+CR group, the number of mucous cells and serous cells was significantly higher than the CY group.
Conclusion: We speculated that CY treatment caused a detrimental effect on the sublingual gland tissues, and that administration of CR also ameliorated the changes induced by CY.

Keywords

Cyclophosphamide, curcumin, sublingual gland, rat

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