Kadmiyum toksisitesi oluşturulan ratlarda arı sütünün farklı dozlarının oksidatif stres ve telomeraz enzimi üzerine etkileri

Year 2022, Volume: 47 Issue: 4, 1516 - 1522, 28.12.2022

Selcen Çakır

https://doi.org/10.17826/cumj.1139113

Abstract

Amaç: Kadmiyum (Cd), çevre kirlenmesi nedeni ile insan sağlığını ciddi oranda tehdit eden endüstride ve tarımda yaygın şekilde kullanılan oksidatif strese ve doku hasarlarına sebep olan toksik bir metaldir. Bu çalışma Cd ile oluşturulmuş doku hasarında arı sütünün (RJ) oksidatif statü ve telomeraz enzim aktivitesine etkisini araştırmayı amaçlamaktadır.
Gereç ve Yöntem: Deneysel tasarım her grupta 6 rat olacak şekilde; Kontrol grubu, Cd grubu, 250 mg/kg RJ, Cd + 250 mg/kg RJ, 400 mg/kg RJ, Cd + 400 mg/kg RJ grubu olacak şekilde 6 guruba ayrılarak oluşturuldu. Çalışmada kan serumundan total oksidan düzeyi, total antioksidan düzeyi kolorimetrik yöntemle ve ovaryum dokusundan telomeraz enzim aktivitesi ELISA yöntemiyle araştırıldı.
Bulgular: Cd oksidatif kapasitede artışa sebep oldu (23.80 ± 2.4) ve RJ uygulamaları ardından kontrol grubuna göre anlamlı azalma izlendi. RJ uygulaması ardından en iyi total antioksidan yanıtı 250 mg/kg RJ ve Cd + 250 mg/kg RJ gruplarında görüldü. Cd telomeraz enzim aktivitesini önemli düzeyde düşürmüştür. (0.90 ± 0.13) Cd uygulaması ardından tedavi amaçlı verilen RJ nin telomeraz düzeylerini yeniden kontrol seviyesine (1.40 ± 0.05) yükselttiği görüldü. Tedaviye en iyi cevap Cd + 250 mg/kg RJ grubunda izlendi (1.42 ± 0.05).
Sonuç: Cd un oksitatif strese sebep olduğu ve RJ uygulamaları ardından antioksidan kapasitede ve telomeraz enzim aktivitesinde görülen artışla RJ nin iyileştirici etkilerinin olabileceği sonucuna ulaşıldı.

Keywords

Arı Sütü, Kadmiyum, Telomeraz, Antioksidan, Ovaryum

Supporting Institution

yok

Project Number

yok

Effects of different doses of royal jelly on oxidative stress and telomerase enzyme in rats with Cadmium toxicity

Abstract

Purpose: Cadmium (Cd) is a toxic metal that seriously threatens human health due to environmental pollution, is widely used in industry and agriculture, and causes oxidative stress and tissue damage. This study aims to examine the effect of royal jelly (RJ) on oxidative status and telomerase enzyme activity in tissue damage induced by Cd.
Materials and Methods: The experimental design was made with 6 rats in each group. A total of 6 groups were created: control group, Cd group, 250 mg/kg RJ group, Cd + 250 mg/kg RJ group, 400 mg/kg RJ group, Cd + 400 mg/kg RJ group. In the study, total oxidant status and total antioxidant status in blood serum were investigated by colorimetric method, and telomerase enzyme activity in ovarian tissue was investigated by ELISA method.
Results: Cd caused an increase in oxidative capacity (23.80 ± 2.4) and a significant decrease was determined after RJ applications compared to the control group. After RJ application, the best total antioxidant response was observed in the 250 mg/kg RJ and Cd + 250 mg/kg RJ groups. Cd significantly reduced telomerase enzyme activity (0.90 ± 0.13). RJ administered for treatment after Cd application increased telomerase levels up to the control level (1.40 ± 0.05). The best treatment response was observed in the Cd + 250 mg/kg RJ group (1.42 ± 0.05).
Conclusion: Cd causes oxidative stress and that RJ may have curative effects by increasing the antioxidant capacity and telomerase enzyme activity RJ is a promising natural product and can contribute to recovery.

Keywords

Cadmium, Royal Jelly, Telomerase, Antioxidant, Ovary

Project Number

yok

References

• REFERENCES 1. Stohs SJ, Bagchi D. Oxidative mechanisms in the toxicity of metal ions. Free Radic Biol Med. 1995;18:321–36.
• 2. Chen L, Liu L, Huang S. Cadmium activates the mitogen-activated protein kinase (MAPK) pathway via induction of reactive oxygen species and inhibition of protein phosphatases 2A and 5. Free Radic Biol Med. 2008;45:1035–44.
• 3. Lee CK, Lee JT, Yu SJ, Kang SG, Moon CS, Choi YH et al. Effects of cadmium on the expression of placental lactogens and Pit-1 genes in the rat placental trophoblast cells. Mol Cell Endocrinol. 2009;298:11–8.
• 4. Thompson J, Bannigan J. Cadmium: Toxic effects on the reproductive system and the embryo. Reprod Toxicol. 2008;25:304–15.
• 5. Cuypers A, Plusquin M, Remans T, Jozefczak M, Keunen E, Gielen H et al. Cadmium stress: An oxidative challenge. BioMetals. 2010;23:927–40.
• 6. Tribowo JA, Arizal MH, Nashrullah M, Aditama AR, Utama DG. Oxidative Stress of Cadmium-Induced Ovarian Rat Toxicity. Int J Chem Eng Appl. 2014;5:254–8.
• 7. Zhang W, Pang F, Huang Y, Yan P, Lin W. Cadmium exerts toxic effects on ovarian steroid hormone release in rats. Toxicol Lett. 2008;182:18–23.
• 8. Blackburn EH. Structure and function of telomeres. Nature. 1991;350:569–73.
• 9. Shay JW, Bacchetti S. A survey of telomerase activity in human cancer. Eur J Cancer Part A. 1997;33:787–91.
• 10. Kim NW. Clinical implications of telomerase in cancer. Eur J Cancer Part A. 1997;33:781–6.
• 11. Hiyama E, Hiyama K. Telomerase as tumor marker. Cancer Lett. 2003;194:221–33.
• 12. Cen J, Zhang H, Liu Y, Deng M, Tang S, Liu W et al. Anti-aging effect of estrogen on telomerase activity in ovariectomised rats - Animal model for menopause. Gynecol Endocrinol. 2015;31:582–5.
• 13. Bekaert S, Derradji H, Baatout S. Telomere biology in mammalian germ cells and during development. Dev Biol. 2004;274:15–30.
• 14. Kanbur M, Eraslan G, Beyaz L, Silici S, Liman BC, Altinordulu Ş et al. The effects of royal jelly on liver damage induced by paracetamol in mice. Exp Toxicol Pathol. 2009;61:123–32.
• 15. Silici S, Ekmekcioglu O, Kanbur M, Deniz K. The protective effect of royal jelly against cisplatin-induced renal oxidative stress in rats. World J Urol. 2011;29:127–32.
• 16. Inoue SI, Koya-Miyata S, Ushio S, Iwaki K, Ikeda M, Kurimoto M. Royal Jelly prolongs the life span of C3H/HeJ mice: correlation with reduced DNA damage. Exp Gerontol. 2003;38:965–9.
• 17. Yang W, Tian Y, Han M, Miao X. Longevity extension of worker honey bees (Apis mellifera) by royal jelly: Optimal dose and active ingredient. PeerJ. 2017;2017.
• 18. Kamakura M. Royalactin induces queen differentiation in honeybees. Nature. 2011;473:478–83.
• 19. Karadeniz A, Simsek N, Karakus E, Yildirim S, Kara A, Can I et al. Royal jelly modulates oxidative stress and apoptosis in liver and kidneys of rats treated with cisplatin. Oxid Med Cell Longev. 2011;2011.
• 20. Collazo N, Carpena M, Nuñez‐estevez B, Otero P, Simal‐gandara J, Prieto MA. Health promoting properties of bee royal jelly: Food of the queens. Nutrients. 2021;13:1–26.
• 21. Cohen J. The statistical power of abnormal-social psychological research: A review. J Abnorm Soc Psychol. 1962;65, 145–153.
• 22. Toupance S, Fattet AJ, Thornton SN, Benetos A, Guéant JL, Koscinski I. Ovarian telomerase and female fertility. Biomedicines. 2021;9:1–17.
• 23. Ansere VA, Ali-Mondal S, Sathiaseelan R, Garcia DN, Isola JVV, Henseb JD et al. Cellular hallmarks of aging emerge in the ovary prior to primordial follicle depletion. Mech Ageing Dev. 2021;194.
• 24. Yan F, Zhao Q, Li Y, Zheng Z, Kong X, Shu C et al. The role of oxidative stress in ovarian aging: a review. J Ovarian Res. 2022;15:1–20.
• 25. Guo J, Wang Z, Chen Y, Cao J, Tian W, Ma B et al. Active components and biological functions of royal jelly. J Funct Foods. 2021;82:104514.
• 26. Ghanbari E, Nejati V, Khazaei M. Antioxidant and protective effects of Royal jelly on histopathological changes in testis of diabetic rats. Int J Reprod Biomed. 2016;14:511–8.
• 27. Hamza RZ, Al-Eisa RA, El-Shenawy NS. Possible Ameliorative Effects of the Royal Jelly on Hepatotoxicity and Oxidative Stress Induced by Molybdenum Nanoparticles and/or Cadmium Chloride in Male Rats. Biology (Basel). 2022;11.
• 28. Yamagata Y, Nakamura Y, Umayahara K, Harada A, Takayama H, Sugino Nmet al. Changes in telomerase activity in experimentally induced atretic follicles of immature rats. Endocr J. 2002;49:589-95.
• 29. Liu X, Jiang C, Chen Y, Shi F, Lai C, Shen L. Major royal jelly proteins accelerate onset of puberty and promote ovarian follicular development in immature female mice. Food Sci Hum Wellness [Internet]. 2020;9:338–45.
• 30. Tesarik J, Galán-Lázaro M, Mendoza-Tesarik R. Ovarian aging: Molecular mechanisms and medical management. Int J Mol Sci. 2021;22:1–10.
• 31. Bellanti F, Matteo M, Rollo T, De Rosario F, Greco P, Vendemiale G et al. Sex hormones modulate circulating antioxidant enzymes: Impact of estrogen therapy. Redox Biol. 2013;1:340–6.
• 32. Nampoothiri LP, Agarwal A, Gupta S. Effect of co-exposure to lead and cadmium on antioxidant status in rat ovarian granulose cells. Arch Toxicol. 2007;81:145–50.