Serum oxidant, antioxidant, and paraoxonase levels in COVID-19 patients

Year 2022, Volume: 47 Issue: 4, 1531 - 1538, 28.12.2022

Rumeysa Duyuran Hüseyin Gürbüz Sinem Bayrakçı Hülya Çiçek

https://doi.org/10.17826/cumj.1167711

Abstract

Purpose: The aim of his study was to determine serum oxidant status (TOS), antioxidant status (TAS), and paraoxonase (PON1) levels and to determine their diagnostic values in patients diagnosed with COVID-19.
Materials and Methods: The research was carried out on patients diagnosed with COVID-19. Within the scope of the study, a total of 87 patients with a diagnosis of COVID-19, 48 (55.1%) male and 39 (44.9%) were evaluated. Total antioxidant determination was performed using a microplate reader according to the Erel method. To calculate the Oxidative stress index (OSI), TOS and TAS levels were determined.
Results: Male gender was associated with high PON1, smoking with high TOS, the presence of hypertension and Diabetes mellitus (DM) diseases with low OSI, and the presence of asthma with low PON1. High PON1 was found to be associated with shorter hospitalization duration and high TOS was associated with longer hospitalization duration. TAS and TOS levels increased significantly due to the increase in CRP, TOS levels due to the increase in neutrophil level, OSI levels due to the increase in leukocyte level, PON1 levels increased due to the increase in LDH level TAS, TOS, OSI, and PON1 cut-off values were 1.41 (AUC: 0.647), 4.56 (AUC: 0.493), 0.421 (AUC: 0.505) and 340 (AUC: 0.536), sensitivity values were 65.5, 55.2, 48.3 and 51.7; specificity values were calculated respectively as 62.1, 46.6, 34.5 and 53.4.
Conclusion: Although it is seen that oxidative stress types have diagnostic value, there is a need for more comprehensive studies with larger samples on the subject.

Keywords

oxidant, antioksidant, paraoxoase, covid-19

COVID-19 hastalarında serum oksidan, antioksidan ve paraoksonaz düzeyleri

Abstract

Amaç: Bu çalışmada, COVID-19 tanısı konan hastalarda serum oksidan, antioksidan ve paraoksonaz düzeylerinin belirlenmesi ve tanısal değerlerinin belirlenmesi amaçlanmıştır.
Gereç ve Yöntem: Araştırma, COVID-19 tanısı konulan hastalar üzerinde gerçekleştirilmiştir. Çalışma kapsamında COVID-19 tanılı 48 (%55,1) erkek ve 39 (%44,9) olmak üzere toplam 87 hasta değerlendirildi. Toplam antioksidan tayini, Erel yöntemine göre bir mikroplaka okuyucu kullanılarak yapıldı. Toplam oksidan tayini, Erel yöntemine göre bir mikroplaka okuyucu kullanılarak yapıldı. OSI hesaplamak için TOS ve TAS seviyeleri belirlendi.
Bulgular: Erkek cinsiyetin yüksek PON1 ile yüksek TOS ile sigara kullanımı, hipertansiyon ve DM hastalıkları varlığı ile düşük OSİ ve düşük PON1 ile astım varlığı ile ilişkili olduğu bulundu. Ayrıca yüksek PON1 daha kısa hastanede kalış süresi ve yüksek TOS daha uzun hastanede kalış süresi ile ilişkili bulunmuştur. CRP'deki artışa bağlı olarak TAS ve TOS seviyeleri, nötrofil seviyesindeki artışa bağlı olarak TOS seviyesi ve OSI seviyesi nedeniyle anlamlı olarak arttı. lökosit seviyesindeki artışa bağlı olarak PON1 seviyesi LDH seviyesindeki artışa bağlı olarak yükselmiştir TAS, TOS, OSI ve PON1 cut-off değerleri 1.41 (AUC: 0.647), 4.56 (AUC: 0.493) olmuştur. 0.421 (AUC: 0.505) ve 340 (AUC: 0.536), hassasiyet değerleri 65.5, 55.2, 48.3 ve 51.7; özgüllük değerleri sırasıyla 62.1, 46.6, 34.5 ve 53.4 olarak hesaplanmıştır.
Sonuç: Çalışmamız sonucunda oksidatif stres türlerinin tanısal değeri olduğu görülmekle birlikte konu ile ilgili daha geniş örneklemli daha kapsamlı çalışmalara ihtiyaç vardır.

Keywords

oksidan, antioksidan, paraoksonaz, covid-19

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