Sıçanlarda deneysel varikosel modelinde D vitamininin etkisi

Year 2024, Volume: 49 Issue: 1, 97 - 103, 29.03.2024

Gökhan Ağtürk , Cemil Tümer , Okan Tutuk , Hatice Doğan , Oğuzhan Özcan , Tümay Özgür , Gülay Gülbol Duran , Muhyittin Temiz

https://doi.org/10.17826/cumj.1395542

Abstract

Amaç: Varikosel, yetişkin erkeklerin yaklaşık %20'sini etkileyen, infertilite ve sperm bozulmasına neden olan patolojik bir durumdur. Çalışmamızın amacı D vitamininin (Vit. D) varikosel patofizyolojisindeki etkinliğini araştırmaktır. Deneysel varikosel modelinde D vitamininin etkinliğini değerlendirmek için biyokimyasal, histopatolojik ve gen ekspresyon analizleri yaptık.
Gereç ve Yöntem: Çalışmada üç gruba ayrılmış 30 yetişkin erkek Wistar albino sıçan kullanıldı: kontrol grubu, deney grubu (varikosel) ve tedavi grubu (varikosel + D vitamini). Tedavi grubuna intramüsküler olarak 500 IU/kg D vitamini verildi.
Bulgular: Deney sonunda toplanan testis ve kan örneklerinde histopatolojik, TRPM2-8 gen ekspresyonu ve biyokimyasal analizler yapıldı. Deney grubunda tübüler yapıda bozulma, total antioksidan seviyelerinde azalma ve total oksidan seviyelerinde artış görüldü. Öte yandan, tedavi grubu TAS'da artış), TOS'da azalma ve tübüler yapı bozukluklarında faydalı bir iyileşme göstermiştir. Gen ekspresyon seviyelerinin analizi TRPM2-8 ekspresyon seviyelerinin varikosel grubunda anlamlı olarak arttığını ve tedavi grubunda azaldığını gösterdi.
Sonuç: Varikosel modelinde, D vitamini kullanımı TRPM2-8 gen seviyesi, patolojik seminifer tübüller ve biyokimyasal değerler üzerinde anlamlı bir etkiye sahipti. Varikosel hastalığında D vitamininin klinik uygulamasını belirlemek için daha ileri çalışmalara ihtiyaç vardır.

Keywords

Varicocele, TRPM, Vitamin D, TAS, TOS

Project Number

Hatay Mustafa Kemal University Scientific Research Projects Unit with project number 16562.

Thanks

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Effect of vitamin D in experimental varicocele model in rats

Abstract

Purpose: Varicocele is a pathological condition that affects approximately 20% of adult men, causing infertility and sperm deterioration. The aim of our study was to investigate the efficacy of vitamin D (Vit. D) in the pathophysiology of varicocele. We performed biochemical, gene expression analyses and histopathological to evaluate the efficacy of vitamin D in the experimental varicocele model.
Materials and Methods: In the study, 30 adult male Wistar Albino rats were used. The rats were divided into 3 groups equally as control group, experimental group (varicocele), treatment group (varicocele + D vit.). The treatment group received 500 IU/kg D vit. intramuscularly.
Results: Histopathological, TRPM2-8 gene expression and biochemical analyses were performed on testicular and blood samples collected at the end of the experiment. The experimental group showed a deterioration in tubular structure, a decrease in total antioxidant levels and an increase in total oxidant levels. The treatment group, on the other hand, showed an increase in TAS, a decrease in TOS and a beneficial improvement in tubular structure disorders. Analysis of gene expression levels showed that TRPM2-8 expression levels were significantly increased in the varicocele group and decreased in the treatment group.
Conclusion: In the varicocele model, the use of vitamin D had a significant effect on TRPM2-8 gene level, pathological seminiferous tubules and biochemical values. Further studies are needed to determine the clinical application of vitamin D in varicocele disease.

Keywords

Varicocele, TRPM, ROS, Vitamin D

Project Number

Hatay Mustafa Kemal University Scientific Research Projects Unit with project number 16562.

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