Protective effects of jervine purified from Veratrum album on paracetamol-induced liver toxicity in rats

Year 2024, Volume: 49 Issue: 1, 150 - 158, 29.03.2024

Serdar Yiğit , Tuba Aydın , Soner Bitiktaş , Seyit Ali Bingöl , Muhammed Yayla Lale Duysak , İsa Yeşilyurt , Ali Alper Kahraman

https://doi.org/10.17826/cumj.1398401

Abstract

Purpose: Since paracetamol toxicity is a very common type of poisoning, we planned to investigate whether Jervine has an effect on paracetamol toxicity by utilizing its anti-inflammatory effect.
Materials and Methods: In our study, 42 Sprague Dawley rats of 8 weeks of age were used. Seven groups were formed with 6 animals in each group. At the 24th hour of the study, all groups underwent laparotomy under anesthesia, and liver dissection was performed. Hematoxylin and Eosin (H&E) staining was performed to evaluate liver histopathology. SOD, CAT, GSH, and MDA levels were analyzed biochemically.
Results: Histopathological, while liver tissues were normal in the control group, we observed degeneration areas, inflammation, and hemorrhage in the paracetamol group. Jervine reduced the severity of paracetamol toxicity and prevented liver damage. Jervine significantly increased SOD levels. Paracetamol administration significantly decreased CAT levels. Paracetamol significantly decreased GSH levels compared to the control group.
Conclusion: Jervine reduced the adverse effects of paracetamol toxicity on liver tissue, such as degeneration, inflammation, and hemorrhage. Jervine increased antioxidant activity and reduced the harmful effects of NAPQI, the toxic metabolite of paracetamol, on liver tissue.

Keywords

Jervine, Veratrum Album, Paracetamol, Antiinflammatory

Ethical Statement

Author Contributions: Concept/Design :SY,MY; Data acquisition:SY; Data analysis and interpretation:TA,LD,AAK; Drafting manuscript:SB; Critical revision of manuscript:İY; Final approval and accountability:SY; Technical or material support:SY; Supervision:SAB; Ethical Approval: With decision 2021-129, the Kafkas University Local Ethics Committee for Animal Experiments gave its consent. Peer-review: Externally peer-reviewed. Conflict of Interest: Authors declared no conflict of interest. Financial Disclosure: The authors have declared that they do not receive financial support

Sıçanlarda parasetamolle indüklenen karaciğer toksisitesi üzerine Veratrum album bitkisinden saflaştırılan jervinin koruyucu etkisi

Abstract

Amaç: Parasetamol toksisitesi de çok yaygın bir zehirlenme tipi olduğundan dolayı bizde jervinin antiinflamatuvar etkisinden faydalanarak parasetamol toksisitesi üzerinde etkisinin olup olmadığını araştırmayı planladık.
Gereç ve Yöntem: Çalışmamızda 42 adet Sprague Dawley cinsi 8 haftalık sıçan kullanıldı. Her grupta 6 dişi hayvan olacak şekilde 7 grup oluşturuldu. Çalışmanın 24. saatinde tüm gruplara anestezi altında laparotomi uygulandı ve karaciğer diseksiyonu yapıldı. Karaciğer histopatolojisini değerlendirmek için Hematoksilen ve Eozin (H&E) boyama yapıldı. Biyokimyasal olarak SOD, CAT, GSH ve MDA seviyelerine bakıldı.
Bulgular: Histopatolojik olarak kontrol grubunda karaciğer dokuları normal iken, parasetamol grubunda dejenerasyon alanları, enflamasyon ve hemoraji gözlemledik. Jervin parasetamol toksisitesinin şiddetini azaltmış ve karaciğer hasarını önlemiştir. Jervin SOD seviyelerini önemli ölçüde artırmıştır. Parasetamol uygulaması CAT seviyelerini önemli ölçüde azaltmıştır. Parasetamol, kontrol grubuna kıyasla GSH seviyelerini önemli ölçüde azaltmıştır.
Sonuç: Çalışmamız, Jervinin parasetamol toksisitesinin karaciğer dokusu üzerindeki dejenerasyon, inflamasyon ve hemoraji gibi olumsuz etkilerini azalttığını ortaya koymuştur. Jervin antioksidan aktiviteyi arttırmış ve parasetamolün toksik metaboliti olan NAPQI'nin karaciğer dokusu üzerindeki zararlı etkilerini azaltmıştı

Keywords

Jervin, Veratrum Album, Parasetamol, Antiiflamtuvar

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