Akut egzersizde antosiyanin uygulamasının redoks dengesi üzerine etkisi: deneysel bir çalışma

Year 2024, Volume: 49 Issue: 2, 471 - 478, 30.06.2024

Seyhan Taşkın , Hakim Çelik , Abdullah Taşkın

https://doi.org/10.17826/cumj.1440184

Abstract

Amaç: Siyanidin-3-glikozit (Cy3G) antioksidan, antidiyabetik, antiinflamatuar ve sitoprotektif özelliklerine atfedilen biyolojik aktivitelerinin çeşitliliği nedeniyle geniş çapta araştırılmaktadır. Bu çalışmada akut egzersizde bozulan redoks dengesi üzerine Cy3G takviyesinin etkilerinin araştırılması amaçlanmıştır.
Gereç ve Yöntem: 28 adet erkek Balb-C fare dört gruba ayrıldı: kontrol, egzersiz, Cy3G ve egzersiz+Cy3G. Deney protokolünün 1-14. günleri boyunca, Cy3G ve Egzersiz+Cy3G gruplarındaki farelere vücut ağırlığının kg'ı başına 5 mg Cy3G verildi. Kontrol ve egzersiz grubundaki fareler standart yem ile beslendi. Farelere akut tükenme egzersizinden önce 2 hafta boyunca 10 m/dak hızda her gün 10 dakika boyunca koşu bandında alıştırma egzersizi yaptırıldı. 15. günde ise akut tükenme egzersizi uygulandı. Ardından tüm gruplar sakrifiye edildi ve serum örneklerinde native thiol, total thiol, disülfit düzeyi ve thiol-disülfit indeksleri analiz edildi.
Bulgular: Egzersizle birlikte verilen Cy3G, native tiyol düzeyini diğer gruplara göre anlamlı düzeyde artırırken, disülfit düzeylerini düşürdüğü bulundu. Total tiyol düzeylerinde herhangi bir değişikliğe neden olmadı. Native tiyol 114.6±64.1, 106.4 ±57.9, 200.1±84.2, 262.1±105.9 olarak ölçüldü; Disülfid düzeyi 253.2±71.6, 257.4±31.5, 213.8±44.8 ve 188.4±32.2 olarak hesaplandı; Total tiyol ise 621.1±116.1, 621.2±100.1, 627.7±125.8, 639.0±105.3 idi (sırasıyla; kontrol, egzersiz, Cy3G ve egzersiz+Cy3G).
Sonuç: Akut egzersizde Cy3G takviyesi, antioksidan savunma sistemlerini destekleyerek ve oksidatif stresi azaltarak potansiyel olarak redoks dengesini koruyabilir. Cy3G kullanımı, spor branşlarında akut yorgunluğa neden olabilecek egzersiz kaynaklı oksidatif stresin önlenmesinde olumlu etki yaratabilir.

Keywords

Antosiyanin, Oksidatif stres, Cy3G, Tiyol, Disülfit

Project Number

TUBITAK Project No: 223S229

The effect of anthocyanin administration on redox balance in acute exercise: an experimental study

Abstract

Purpose: Due to the diverse biological activities attributed to its antioxidant, antidiabetic, anti-inflammatory, and cytoprotective properties, cyanidin-3-glucoside (Cy3G) has been widely studied. The present study was conducted to investigate the effects of Cy3G supplementation on redox balance disruption during acute exercise.
Materials and Methods: To this end, 28 male Balb-C mice were divided into four groups: control, exercise, Cy3G, and exercise+Cy3G. During the experimental period, the mice in the Cy3G and exercise+Cy3G groups were administered 5 mg of Cy3G per kg of body weight, while the control and exercise groups were fed standard chow. The mice were trained on treadmill for 10 min every day at speed of 10 m/min for 2 weeks. On the 15th day, an acute exhaustion exercise was applied. Then, all groups were sacrificed, and serum samples were taken to analyze the native thiol, total thiol, disulfide, and thiol-disulfide indices.
Results: The findings showed that the combination of Cy3G and exercise significantly increased native thiol levels and decreased disulfide levels compared to the other groups. However, it did not cause any change in total thiol levels. Native thiol were measured as 114.6±64.1, 106.4 ±57.9, 200.1±84.2, 262.1±105.9; Disulfide were determined as 253.2±71.6, 257.4±31.5, 213.8±44.8 and 188.4±32.2; Total thiol were 621.1±116.1, 621.2±100.1, 627.7±125.8 and 639.0±105.3 (respectively; control, exercise, Cy3G, and exercise+Cy3G).
Conclusion: Cy3G supplementation can potentially maintain redox balance during acute exercise by supporting antioxidant defense systems and reducing oxidative stress. The use of Cy3G may have a positive effect on preventing exercise-induced oxidative stress and acute fatigue.

Keywords

Anthocyanin, Oxidative stress, Cy3G, Thiol, Disulfide

Ethical Statement

The study protocol was approved by the Harran University Animal Experiments Local Ethics Committee (No: 2023/006/03) and conformed to the Guide for the Care and Use of Laboratory Animals.

Supporting Institution

This study was supported by Scientific and Technological Research Council of Türkiye (TUBITAK) (Project No: 223S229)

Project Number

TUBITAK Project No: 223S229

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