Plevral efüzyonlarda transude ile eksuda ayrımında interlökin-6 geninin rolü

Year 2024, Volume: 49 Issue: 2, 391 - 399, 30.06.2024

Müge Gülcihan Önal , Hilal Akalın , Armagan Akkuş , Munis Dündar , Ömer Önal

https://doi.org/10.17826/cumj.1441437

Abstract

Amaç: Bu çalışma, plevral sıvı örneklerinde IL-6 gen ekspresyonunu değerlendirmeyi ve ekspresyon düzeyleri ile promotör polimorfizmleri arasındaki ilişkiyi inceleyerek plevral efüzyon sınıflandırmasında daha doğru sonuçlar elde etmeyi amaçlamaktadır.
Gereç ve Yöntem: Çalışmaya plevral sıvısı bulunan toplam 38 yetişkin hasta (19 transüda, 19 eksüda) ve 33 sağlıklı kontrol grubu dahil edildi. IL-6 gen ekspresyonu çalışması için, transüda ve eksüda plevral sıvılarından RNA izole edildi ve ekspresyon seviyeleri iki grup arasında karşılaştırıldı. Ardından, kontrol ve hasta gruplarının genomik DNA'larından LightCycler 480 II real-time polimeraz zincir reaksiyonu kullanılarak -174G>C (rs1800795), -572G>C (rs1800796) ve -597G>A (rs1800797) polimorfizmleri analiz edildi.
Bulgular: Eksüda grubunda IL-6 düzeyleri, transüda grubuna göre 7,13 kat daha fazla ifade edildi. Transüda-eksüda grupları arasında polimorfizmler açısından anlamlı bir fark bulunamadı. Ancak transüda-eksüda hasta grupları kontrol grupları ile karşılaştırıldığında, -174G>C polimorfizmi ve -597G>A polimorfizmi istatistiksel olarak anlamlıydı.
Sonuç: Plevral efüzyon tedavisi sıvı karakterizasyonu ile başlar. Zorlu vakalarda, mevcut parametreler yetersizdir. Bulgularımız, geleneksel kriterlerin ötesine geçerek eksüdatif ve transüdatif durumları bağımsız olarak ayırt eden güçlü bir biyobelirteç olarak IL-6 'yı işaret etmektedir. IL-6 , plevral efüzyonun hassas karakterizasyonu için umut vermekte olup, patofizyoloji hakkında bilgi sağlayarak hedeflenen tedavi yaklaşımlarına olanak tanımaktadır.

Keywords

IL-6, mRNA ekspresyonu, plevral efüzyon, genetik polimorfizm

Ethical Statement

Our study was approved by Erciyes University Ethics Committee (No: 2017/262). Erciyes University Ethics Committee approved this project according to the Declaration of Helsinki revised in 2000.

Supporting Institution

Erciyes University Scientific Research Projects Unit (Kayseri, Turkey)

Thanks

This study was supported by Erciyes University Scientific Research Projects Unit (Kayseri, Turkey) with the number TDK-2017-7518.

The role of interleukin-6 gene in distinction of transudate-exudate in pleural effusions

Abstract

Purpose: This study aims to appraise IL-6 gene expression in pleural fluid samples and establish a connection between expression levels and promoter polymorphisms for more accurate pleural effusion classification.
Materials and Methods: A total of 38 adult patients (transudate (19) and exudate (19)) with pleural fluid and 33 healthy controls were included in the study. For the IL-6 gene expression study, RNA was isolated from transudate and exudate pleural fluids, and expression levels were compared between the two groups. Then, -174G>C (rs1800795), -572G>C (rs1800796) and, -597G>A (rs1800797) polymorphisms were analyzed using LightCycler® 480 II with real-time polymerase chain reaction from genomic DNA of controls and patients.
Results: IL-6 levels were 7.13-fold more expressed in the exudate group than in the transudate group. No significant difference was found between the transudate-exudate groups in terms of polymorphisms. However, when we compared the transudate-exudate patient groups with the control groups, -174 G>C polymorphism and -597 G>A polymorphism were statistically significant.
Conclusion: Pleural effusion treatment initiates with fluid characterization. In challenging cases, the current parameters are inadequate. Our findings indicate that IL-6 is a robust biomarker, independently distinguishing exudative and transudative states, surpassing traditional criteria. IL-6 shows promise for precise pleural effusion characterization, offering insights into pathophysiology and enabling targeted therapeutic interventions.

Keywords

IL-6, mRNA Expression, Pleural Effusion, Genetic Polymorphism

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