Wistar sıçanlarda tadalafilin miyokard üzerindeki etkilerinin elektriksel ve izometrik kasılmalar ve kardiyak belirteçler yoluyla araştırılması

Year 2024, Volume: 49 Issue: 3, 639 - 645, 30.09.2024

Duygun Altıntaş Aykan , Selma Yaman , Muhammed Seyithanoğlu , Ahmet Çağrı Aykan

https://doi.org/10.17826/cumj.1457346

Abstract

Amaç: Uzun etkili bir fosfodiesteraz-5 (PDE-5) inhibitörü olan tadalafil, erektil disfonksiyon tedavisinde yaygın olarak kullanılmaktadır. Bu çalışma, sıçanlarda miyokardın izometrik ve elektriksel kasılmalarını ölçerek tadalafilin kalp fonksiyonuna katkısını araştırmaktadır.
Gereç ve Yöntem: Otuz sıçan beş gruba ayrıldı. Bazal elektriksel kasılmalar elektrokardiyogram (EKG) aracılığıyla PR (ms), QRS (ms), QT (ms), Tp (ms), Te (ms), patolojik Q ve kalp atım/dakika aralıklarında kaydedildi. Daha sonra grup 1'e 7 gün boyunca salin verildi (kontrol); grup 2'ye 1 mg/kg tadalafil verildi; grup 3'e 10 mg/kg tadalafil verildi; grup 4'e 7 gün süreyle 1 mg/kg tadalafil verildi; grup 5'e 7 gün süreyle 10 mg/kg tadalafil verildi. Tedavilerden sonra EKG'deki farklılıkları analiz etmek için elektriksel kasılmalar yeniden gerçekleştirildi. İzometrik kasılmalar için kalpler, miyokardiyal kasılma kuvvetlerini (g), sürelerini (ms) ve frekanslarını (Hz) belirlemek için izometrik güç dönüştürücüye bağlandı. ELISA yoluyla kardiyak kreatin kinaz (CK-MB) ve kardiyak troponin I için serum örnekleri toplandı.
Bulgular: CK-MB değerlerinde Grup 2'de (395,56±124,38 pg/ml) ve Grup 3'te (377,81±79,61 pg/ml), Grup 1'e (575,32±83,54 pg/ml) göre anlamlı bir azalma bulduk. Kardiyak kasılma kuvvetleri, kasılma süreleri veya frekanslarındaki farklılıklar istatistiksel olarak anlamlı değildi.
Sonuç: Tadalafil, miyokardın elektriksel veya izometrik kasılmaları üzerinde belirgin bir bozulma yaratmadı. Tadalafil 1 ve 10 mg/kg'ın serum CK-MB'yi azalttığı dikkat çekicidir. QT'deki kısalma ve kalp hızındaki azalmanın miyokardiyal fonksiyonlar üzerinde önemli etkileri olabilir.

Keywords

Tadalafil, Kas kontraksiyonu, Miyokard, Elektrokardiyografi

Ethical Statement

All experiments were conducted in accordance with the National Institute of Health Guidelines for the Care and Use of Laboratory Animals. Experimental protocols were approved by Kahramanmaraş Sütçü İmam University Local Animal Experimentation Ethics Committee (File No: 2019/01/04, Approval date: 19.02.2019).

Investigation of the effects of tadalafil on the myocardium via electrical and isometric contractions and cardiac markers in Wistar rats

Abstract

Purpose: Tadalafil, a long-acting phosphodiesterase-5 (PDE-5) inhibitor, is commonly used in the treatment of erectile dysfunction. This study investigates the contribution of tadalafil in cardiac function by measuring isometric and electrical contractions of myocardium in rats.
Materials and Methods: Thirty rats were divided into five groups. Basal electrical contractions were recorded via electrocardiogram (ECG) for intervals PR (ms), QRS (ms), QT (ms), Tp (ms), Te (ms), pathological Q and heart beats/min. Then group 1 received saline for 7 days (control); group 2 received tadalafil 1 mg/kg; group 3 received tadalafil 10 mg/kg; group 4 received tadalafil 1 mg/kg for 7 days; group 5 received tadalafil 10 mg/kg for 7 days. After treatments, electrical contractions were re-performed to analyze the differences in ECG. For isometric contractions, hearts were connected to isometric power transducer to determine myocardial contractile forces (g), durations (ms) and frequencies (Hz). Serum samples were collected for cardiac creatine kinase (CK-MB) and cardiac troponin I via ELISA.
Results: We found a significant decrease in CK-MB in Group 2 (395.56±124.38 pg/ml) and 3 (377.81±79.61 pg/ml), compared to Group 1 (575.32±83.54 pg/ml). The differences in cardiac contractile forces, contraction durations or frequencies were not statistically significant.
Conclusion: Tadalafil did not exert obvious distruption on myocardial electrical or isometrical contractions. It is noteworthy that tadalafil 1 and 10 mg/kg reduced serum CK-MB. Shortening in QT and decrease in heart rate may have important implications on myocardial functions.

Keywords

Tadalafil, Muscle contraction, Myocardium, Electrocardiography

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