Evaluation of the protective and therapeutic effect of Salvia officinalis against testicular damage caused by docetaxel in rats

Year 2025, Volume: 50 Issue: 1, 137 - 147, 31.03.2025

Mustafa Öztürk , Demet Bolat , Arzu Yay , Perihan Gürbüz , Münevver Baran

https://doi.org/10.17826/cumj.1607896

Abstract

Purpose: This study aims to investigate the possible side effects of the chemotherapeutic agent Docetaxel on the male reproductive system and in vivo the preventive or therapeutic role of the hydroalcoholic extract of S. officinalis L. against these effects.
Materials and Methods: In the study, 50 Wistar albino male rats were divided into five groups: Control; Docetaxel; S. officinalis; S. officinalis + Docetaxel; and Docetaxel + S. officinalis groups. After the treatments, the testicular tissues of the rats were excised and fixed in 10% formaldehyde. After routine tissue processing, sections were taken. Then, hematoxylin-eosin and immunohistochemical staining (PGP 9.5 and DAZL1) were performed, and immunoreactivity intensity was evaluated.
Results: In the light microscopic examinations of the preparations to which the routine histological tissue tracking method was applied, the testicular architecture was observed to be normal in the control group, while pathological changes such as irregularity, hemorrhage, and spermatogenic cells spilling into the lumen were observed in the seminiferous tubule epithelium in the docetaxel group. In the evaluation made according to the Johnsen scoring system, a significant decrease was detected in the docetaxel group compared to the control group, and the groups applied only to S. officinalis. According to the analysis of the immunoreactivity intensities of PGP 9.5 and DAZL1, it was seen that Sal applied before docetaxel application showed protective properties on the testicular tissue and increased the expression levels compared to the Dox + Sal group
Conclusion: This study shows that S. officinalis 70% methanol (MeOH) extract may protect against the negative effects of docetaxel on testicular tissue. Our findings are promising because they indicate that when given to patients before dosetaxel administration, the distinct protective characteristics of S. officinalis may provide a novel therapeutic option. It is also thought that it may contribute to future in vitro studies examining the effects of S. officinalis on the reproductive system.

Keywords

Docetaxel, Salvia officinalis, Testis, Rat

Ethical Statement

Approval was obtained from the Erciyes University Animal Experiments Local Ethics Committee (Decision No: 21/164).

Supporting Institution

Erciyes University

Project Number

This study was supported by Erciyes University Scientific Research Project Coordination Unit (Project no: EUBAP, TYL-2021-11448).

Thanks

Scientific Research Projects (BAP) and Experimental Research Application and Research Center - DEKAM

Sıçanlarda dosetakselin neden olduğu testis hasarına karşı Salvia officinalis’in koruyucu ve terapötik etkisinin değerlendirilmesi

Abstract

Amaç: Bu çalışma ile kemoterapötik ajan olan Dosetakselin erkek üreme sistemi üzerindeki olası yan etkileri ve bu etkilere karşı S. officinalis L. bitkisinden hazırlanan hidroalkolik ekstresinin koruyucu veya tedavi edici rolününün in vivo olarak araştırılması amaçlanmıştır.
Gereç ve Yöntem: Çalışmada, 50 adet Wistar albino erkek sıçan beş gruba ayrılmıştır: Kontrol, Dosetaksel, S. officinalis, S. officinalis + Dosetaksel ve Dosetaksel + S. officinalis grubu. Uygulamalar sonrasında sıçanların testis dokuları eksize edilmiş ve %10’luk formaldehitte fikse edilmiştir. Rutin doku takip işlemlerinin ardından kesitler alınmıştır. Daha sonra Hematoksilen-Eozin ve immünohistokimyasal boyamalar (PGP 9,5 ve DAZL1) yapılmış ve immunreaktivite yoğunlukları değerlendirilmiştir.
Bulgular: Rutin histolojik doku takip yöntemi uygulanan preparatların ışık mikroskobik incelemelerinde kontrol grubunda testis mimarisinin normal olduğu gözlenirken, docetaxel grubunda seminifer tübül epitelinde düzensizlik, hemoraji ve spermatogenik hücrelerin lümene dökülmesi gibi patolojik değişiklikler izlendi. Johnsen skorlama sistemine göre yapılan değerlendirmede docetaxel grubunda kontrol grubuna ve sadece S. officinalis uygulanan gruplara göre anlamlı azalma saptand). PGP 9.5 ve DAZL1'in immunoreaktivite yoğunluklarının analizine göre docetaxel uygulamasından önce uygulanan Sal'ın testis dokusu üzerinde koruyucu özellik gösterdiği ve Dox + Sal grubuna göre ekspresyon düzeylerini artırdığı görüldü .
Sonuç: Bu çalışma, S. officinalis %70 metanol (MeOH) ekstresinin docetaxel'in testis dokusu üzerindeki olumsuz etkilerine karşı koruma sağlayabileceğini göstermektedir. Bulgularımız umut vericidir çünkü dosetaxel uygulamasından önce hastalara verildiğinde, S. officinalis'in belirgin koruyucu özelliklerinin yeni bir tedavi seçeneği sağlayabileceğini göstermektedir. Ayrıca, S. officinalis'in üreme sistemi üzerindeki etkilerini inceleyen gelecekteki in vitro çalışmalara katkıda bulunabileceği düşünülmektedir.

Keywords

Dosetaksel, Salvia officinalis, Testis, Sıçan

Ethical Statement

Erciyes Üniversitesi Hayvan Deneyleri Yerel Etik Kurulu’ndan onay alındı ​​(Karar No: 21/164).

Supporting Institution

Erciyes Üniversitesi

Project Number

This study was supported by Erciyes University Scientific Research Project Coordination Unit (Project no: EUBAP, TYL-2021-11448).

Thanks

Bilimsel araştırma Projeleri (BAP) ve Deneysel Araştırmalar Uygulama ve Araştırma Merkezi - DEKAM' a

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