Safran-withania somnifera bitkisel jelinin ağız ülserlerine karşı antioksidan etkinliğinin değerlendirilmesi: bir invitro çalışma

Year 2025, Volume: 50 Issue: 2, 261 - 269, 30.06.2025

Madhumitha Mahalingam , Devika S Pıllaı , Rajeshkumar Shanmugam

https://doi.org/10.17826/cumj.1563879

Abstract

Öz
Amaç: Tekrarlayan aftöz stomatit (RAS), oral mukozanın en sık görülen ülseratif lezyonudur. Lezyonlar genellikle tekrarlayan, ağrılı olup yaşam kalitesini etkileyebilir. RAS’ta antioksidan ajan olarak safran, withania somnifera, kuru zencefil, tulsi bitkisel ekstraktlarının etkinliğini değerlendirmek.
Gereç ve Yöntem: 1 g tulsi, withania somnifera, kuru zencefil tozu, 100 ml damıtılmış su ile ayrı ayrı karıştırıldı ve 50 ila 60 derecede bir ısıtma mantosunda 15 dakika kaynatıldı. Karboksilat metil selüloz 5 g, karbapel 5 g kombinasyonunu içeren boş jele 5 ml ekstrakt eklenerek antiinflamatuar, antimikrobiyal, antioksidan, sitotoksik aktiviteler test edildi. Withania somnifera, safran, kuru zencefil ve fesleğenin antioksidan özelliği DPPH testi, H2O2 testi ve FRAP testi kullanılarak değerlendirildi.
Bulgular: Çalışmamız, mililitre başına 50 mikrogram konsantrasyonda %90.22, 84.3, 88.93'lük en yüksek inhibisyon bölgesini ortaya koydu; bu, standart değere yakın bir değerdir.
Sonuç: Bu çalışma, tıbbi bitkilerin doğal olarak oluşan bileşenlerinin ağız ülserlerini çözebileceği ve böylece hastaların yaşam kalitesini artırabileceği konusunda bir fikir vermektedir. Dolayısıyla, bitkisel ilaçlar ticari olarak temin edilebilen sentetik ilaçlara ek olarak kullanılabilir.

Keywords

Withania somnifera , tulsi , kuru zencefil , safran , ülser , ilaç , yaşam kalitesi

Ethical Statement

Scientific Review Board- Saveetha Dental College number for the study entitled "ASSESSMENT OF ANTIOXIDANT PROPERTY OF SAFFRON BASED HERBAL GEL AGAINST RECURRENT APHTHOUS STOMATITIS- AN INVITRO STUDY " is SRB/SDC/OMED-2204/23/136

Project Number

SRB/SDC/OMED-2204/23/136

Assessment of antioxidant efficacy of saffron-withania somnifera herbal gel against oral ulcers: an invitro study

Abstract

Abstract
Purpose: Recurrent aphthous stomatitis (RAS) is the most commonly seen ulcerative lesion of the oral mucosa. The lesions are generally recurrent, painful and can affect the quality of life. The aim of this study was to evaluate the efficacy of herbal extracts of saffron, withania somnifera, Dry ginger, Tulsi as an antioxidant agent in RAS.
Materials and Methods: 1g powder of tulsi, withania somnifera, dry ginger was mixed with 100ml of distilled water separately and boiled in a heating mantle at 50 to 60 degree for 15 minutes. Anti-inflammatory, antimicrobial, antioxidant,cytotoxic activities were tested by adding 5 ml extract to empty gel containing a combination of carboxylate methyl cellulose 5g, carbapel 5g. Antioxidant property of withania somnifera, saffron, dry ginger and tulsi were evaluated using DPPH assay, H2O2 assay and FRAP assay.
Results: Our study revealed highest zone of inhibition of 90.22, 84.3, 88.93% at a concentration of 50 microgram per milliliter which is near to that of standard value
Conclusion: This study gives an insight that the naturally occurring constituents of medicinal herbs will be able to resolve oral ulcers thereby improving the quality of life of the patients. Hence, herbal medicines can be used as an adjunct to synthetic drugs available commercially.

Keywords

Withania somnifera , Tulsi , Dry ginger , Saffron , ulcer , medicine , quality of life

Project Number

SRB/SDC/OMED-2204/23/136

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