Matrigel ve kolajen I hepatosit karsinomu hücre davranışını etkiler: konfluense bağımlı çalışma

Year 2025, Volume: 50 Issue: 3, 899 - 907, 30.09.2025

Zeynep Akbulut , Can Daylan , Gamze Demirel

https://doi.org/10.17826/cumj.1722027

Abstract

Amaç: Tümör mikroçevresi, hepatosellüler karsinom (HCC) ilerlemesini önemli ölçüde etkiler ve epitel-mezenkimal geçiş (EMT) metastazın temel itici gücüdür. Bu çalışma, iki önemli ECM bileşeni olan Matrigel ve Tip I kollajenin HepG2 karaciğer kanseri hücre davranışını düzenlemedeki farklı rollerini araştırmaktadır
Gereç ve Yöntem: HepG2 hücreleri tip I kollajen ve Matrigel kültür ortamında kültürlendi ve YAP1, Na-K ATPaz ve E-kadherin ifadesi konfokal mikroskop kullanılarak araştırıldı. Hücre birleşmesinin etkisini göz önünde bulundurarak bunların proliferasyon ve ifadesi üzerindeki etkileri araştırıldı.
Bulgular: HepG2 hücreler en yüksek proliferasyonu Tip I kolajen ortamda konfluent sonrası kültür zamanda göstermiştir. YAP1 ifadesi her iki kültür zamanında Tip I kolajenli ortamda yüksek çıkmış (ve tam konfluente ulaştıkları zamanda YAP1 lokalizasyonu sitoplazmadan çekirdeğe doğru olmuştur. Na-K ATPaz ekspresyonu, kontrol hücrelerde konfluent öncesi yüksekken, konfluent sonrası matrigel ortamda artış göstermiştir. E-kadherin, Tip I kolajen ortamda kontrol göre istatistiksel olarak azalma göstermiş, matrigel kültürlü hücrelerde konfluent sonrası dönemde ifadesi yüksek bulunmuştur.
Sonuç: Bu bulgular, ekstrasellülar matrix bileşenlerinin HCC hücre çoğalması ve EMT ile ilişkili protein ifadesi üzerindeki farklı etkilerini vurgulayarak, tümör mikroçevresi içinde potansiyel terapötik hedeflere işaret etmektedir.

Keywords

Ekstrasellüler matriks , epitel-mezankimal geçiş , matrigel , tip 1 kolajen

Ethical Statement

Çalışma hücre kültürü çalışmasıdır, Etik Kurul onayı gerekmemektedir.

Supporting Institution

Tübitak 2209A Üniversite Öğrencileri Araştırma Projeleri Destekleme Programı

Matrigel and collagen I impact hepatocellular carcinoma cell behavior: a confluency-dependent study

Abstract

Purpose: The tumor microenvironment significantly influences hepatocellular carcinoma (HCC) progression, with epithelial-mesenchymal transition (EMT) being a key driver of metastasis. This study explores the distinct roles of matrigel and type I collagen, two prominent ECM components, in modulating HepG2 liver cancer cell behavior.
Materials and Methods: HepG2 cells were cultured in type I collagen and Matrigel culture media, and the expression of YAP1, Na-K ATPase, and E-cadherin were investigated using a confocal microscope. We examine their impact on proliferation and the expression of, considering the influence of cell adherent.
Results: HepG2 cells showed the highest proliferation at post-confluent culture time in type 1 collagen medium. YAP1 expression was high in type 1 collagen medium at both culture times (and when they reached adherent, YAP1 localization was from the cytoplasm to the nucleus. Na-K ATPase expression was high in control cells non-adherent, while it increased in Matrigel adherent. E-cadherin showed a statistically significant decrease in type 1 collagen medium compared to control and its expression was found to be high in Matrigel cultured cells adherent.
Conclusion: These findings highlight the differential effects of extracellular matrix components on HCC cell proliferation and EMT-related protein expression, suggesting potential therapeutic targets within the tumor microenvironment.

Keywords

Collagen type I , extracellular matrix , epithelial-mesenchymal transition , matrigel

Ethical Statement

The study is a cell culture study. Ethical committee permission is not required.

Supporting Institution

Scientific and Technological Research Council of Turkey

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