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Effects of the Food Additive Sodium Benzoate on Developing Chicken Liver

Year 2016, Volume: 37 Issue: 2, 85 - 98, 20.04.2016

Yücel Başimoğlu Koca , Feryal Karakahya

https://doi.org/10.17776/csj.29808

Abstract

Abstract. In this study, the effects of sodium benzoate (E-211) (SB) on the liver tissues of chicken embryos which were exposed to different doses of SB and for different durations were histopathologically investigated. SB was injected into vitellus at three different doses (250, 500, 1000 mg/kg) on the 5th day of the incubation. The embryos of the control and experimental groups were collected on the 7th day (stage 31) and 10th day (stage 36) of incubation and weighed. It was statistically detected that SB caused rise to a significant decrease (P<0.001) on the total weights of the embryos depending in the dose and duration. At microscopic level deterioration of vein structures, congestion, edema, sinusoidal dilation, deterioration of hepatocyte arrangements, swelling, vacuolization, chromatin condensation, karyolysis, shape deformation and eccentric located of the nuclei decrease in Nuclear Organization Regions (NOR) and mitotic division, stickiness of chromosomes, anaphase bridge, nuclei budding and the formation of micronuclei were observed.

Keywords: Food additive, sodium benzoate, chicken embryo, liver, histopathology

 

Özet. Bu çalışmada sodyum benzoata (E211) farklı doz ve sürelerle maruz kalan tavuk embriyosu karaciğer dokusuna etkileri histopatolojik yönden araştırılmıştır. Sodyum benzoat, inkübasyonun 5. gününde üç ayrı dozda (250, 500, 1000 mg/kg) vitellusa enjekte edildi. Kontrol ve deney gruplarına ait embriyolar inkübasyonun 7. (safha 31) ve 10. günlerinde (safha 36) çıkarıldı, ağırlıkları tartıldı. Yapılan istatistiki değerlendirmeler sonucunda sodyum benzoatın doza ve süreye bağlı olarak embriyoların toplam ağırlıklarında anlamlı bir azalmaya neden olduğu belirlendi (P<0.001). Mikroskop düzeyinde venlerin yapısında bozulma, konjesyon, ödem, sinuzoidlerde genişleme, hepatosit düzenlenmelerinde bozulma, şişme, vakuolizasyon, kromatin kondensasyonu, karyolisis, nükleusun şekil bozukluğu ve eksentrik yerleşimi, Nükleolar Organizasyon Bölgeleri’nde (NOR) ve mitotik bölünmede azalma, kromozomlarda yapışıklık, kalgın kromozomlar, nükleusta tomurcuklanma ve mikronükleus oluşumu gözlendi.

Anahtar Kelimeler: Gıda katkısı, sodyum benzoat, tavuk embriyo, karaciğer, histopatoloji

References

  • Xue, B., Wang, H.F., Xu, L.H., Sun, H.F., Liu, Y.F., Peng, S.X., Liu, KX., Guo, Z.Y.,2004. Benzoate derived DNA adduction and its decay in mice measured by AMS. Nucl Instr Meth Phys ResB. 223-224: 765-69.
  • Türkoğlu, Ş., 2007. Genotoxicity of five food preservative stested on roottips of Allium cepaL. Mutat Res. 626: 4-14.
  • Chen, Q., Huang, N., Huang, J., Chen, S., Fan, J., Li, C., Xie, F.K., 2009. Sodium benzoate exposure down regulates the expression of tyrosine hydroxylase and dopamine transporter in dopaminergic neurons in developing zebrafish. Birth Defects Res (Part B). 86: 85-91.
  • Fujitani, T., 1993. Short-term effect of sodium benzoate in F344 rats and B6C3F1 mice. Toxicol Letters. 69: 171-79.
  • Sinha, R., D’Souza, D., 2010. Liver cell damage caused due to sodium benzoate toxicity in mice. Int J Biotech Biochem. 6(4): 549-54.
  • FDA (Food and Drug Administration): Food ingredients and packaging terms. Food Additive. See: Avaliable from: http://www.fda.gov/Food/FoodIngredientsPackaging/ucm064228.htm– Accessed:20.05.2010
  • Food and Organization (FAO): Sodium benzoate. http://www.fao.org/ag/agn/jecfa- additives/specs/Monograph1/Additive-393.pdf – Accessed: 20.05.2010.
  • Srour R: Benzoic acid and derivatives. Aromatic intermediat esandderivative. Paris, A.IV.1– A.IV.17, 1998.
  • RxMED: The website for family, physicians and you. The comprehensive recource for physicians, drug and illness information. See: Avaliable from: http://www.rxmed.com/b.main/b2.pharmaceutical/b2.1.monographs/CPS– Accessed: 20.11.2012. [10]. [10] EWG’s Skin Deep Cosmetics Database: Avaliable at:http://www.cosmeticsdatabase.com– Accessed: 20.05.2010.
  • Baldwin, E.A., Nisperos-Carriedo, M.O., Baker, R.A., 1995. Use of edible coatings to preserve quality of lightly (and slightly) processed products. Crit Rev Food Sci Nutr,35(6): 509-24.
  • World Health Organization (WHO):World health organization coincises international assessment document 26, benzoic acid and sodium benzoate. Genova, Switzerland. See: Avaliable from:http://www.who.int/ipcs/publications/cicad/cicad26_rev_1.pdf– Accessed: 22.09.2011.
  • Dawson, D.A., Schultz, T.W., Hunter, R.S., 1996. Developmental toxicity of carboxylic acids to Xenopus embryos: A quantitative structure activity relationship and computer- automated structure evaluation. Teratog Carcinog Mutage.16: 109-124.
  • Tsay, H.J., Wang, Y.H., Chen, W.L., Huang, M.Y., Chen, Y.H., 2007. Treatment with sodium benzoate leads to malformation of zebra fish larvae. Neurotoxicol Teratol.29: 562- 69.
  • Onodera, H., Ogiu, T., Matsuoka, C., Furuta, K., Takeuchi, M., Oono, Y., Kubota, T., Miyahara, M., Maekawa, A., Odashima, S., 1978. Studies on effects of sodium benzoate on fetuses and offspring of wistar rats. Eisei Shikenjo Hokoku.96: 47-55.
  • Concise International Chemical Assessment Document (CICAD)(2000). Benzoic acid and sodium benzoate. Document: 26. See: Available from: http://www.inchem.org/documents/cicads/cicads/cicad26.htm– Accessed:24.10.2010.
  • Hamburger, V., Hamilton, H.L., 1992. A series of normal stages in the development of chick embryo. Dev Dynam.195: 231-72.
  • Bancroft, J.D., Cook, H.C., 1994. Manual of Histological Techniques and Their Diagnostic Application. 457 p, New York, Churchill Livingstone.
  • Minor, J.L., Becker, J.A., 1971. A comparison of the teratogenic properties of sodium salicylate, sodium benzoate and phenol. Toxicol Appl Pharm. 19: 373.
  • Kaboğlu, A., Aktaç, T,A., 2002. Study of the effects of the sodium benzoate on the mouse liver. Biol Brat. 57(3): 373-80.
  • Çalışır Erden, Z., Çalışkan, D., 2003. Gıda katkı maddeleri ve insan sağlığı üzerine etkileri. Ankara Ecz Fak Derg. 32(3): 193-206.
  • Lahijani, H.A.K., Rahimpour-Bonab, H., Tavakoli, V., Hosseindoost, M., 2009. Evidence for late holocene highstands in central Guilan. Quatern Int. 197: 55-71.
  • De Robberties, E.D.P., De Robberties, E.M.F., 1980. Cell and molecular biology. INFO- MED. Int. Ed.
  • El-Elaimy, I.A., Sakr, S.A., El-Sadeny, M.M., Gabr, S.A., 1993. Electron microscopic study of the liver of Tilapia exposed to neopybuthrin. Bull Environ Contam Toxicol. 50: 682-88.
  • Nair, B., 2001. Cosmetic ingredient review expert panel “Final report on the safety assessment of benzyl alcohol, benzoic acid and sodium benzoate”. Int J Toxicol. 3: 23-50.
  • Zengin, N., Yüzbaşıoğlu, D., Ünal, F., Yılmaz, S., Aksoy, H., 2011. The evaluation of the genotoxicity of two food preservatives: Sodium benzoateand potassium benzoate. Food Chem Toxicol. 49: 763-69.
  • Sasaki, Y.F., Kawaguchi, S., Kamaya, A., Ohshita, M., Kabasawa, K., Iwama, K., Taniquchi, K., Tsuda, S., 2002. The coment assay with 8 mouse organs: result with 39 currently used food additives. Mutat Res. 519: 103-19.
  • Sarıkaya, R., Solak, K., 2003. Genotoxicity of benzoic asit studied in the Drosophila melanogaster somatic mutation and recombination test (SMART).Gazi Eğt Fak Derg. 23(3): 19-32.
  • Mpountoukas, P., Vantarakis, A., Sivridis, E., Lialiaris, T., 2008. Cytogenetic study in cultured human lymphocytes treated with three commonly used preservatives. Food Chem Toxicol,46(7): 2390-93.
  • Yılmaz, S., Ünal, F., Yüzbaşıoğlu, D., 2009. The in vitro genotoxicity of benzoic acid in
  • human peripheral blood lymphocytes. Cytotechn. 60: 55-61.
  • Darnell, J., Lodish, H., Baltimore, D., 1986. Molecular cell biology. Scientific American Books New York, p269-369.
  • Egan, M.J., Crocker, J., 1992. Nucleolar organizer regions in pathology. British Journal of Cancer.65: 1-7.
  • Giri, D.D., Nottingham, J.F., Lawry, J., Dundas, S.A., Underwood, J.C.E., 1989. Silver binding nucleolar organiser regions (agnors) in benign and malignant breast lesions: correlations with ploidy and growth phase by DNA flow cytometry. J Path. 157: 307-13.
  • Chern, J.H., Lee, Y.C., Yang, M.H., Chang, S.C., Perng, R.P., 1997. Usefulness of argyrophilic nucleolar organiser regions score to differentiate suspicious malignancy in pulmonary cytology. Chest. 111: 1591-96.
  • Lee, J.S., Lee, M.S., Park, C.S., Juhng, S.W., 1997. Diagnostic value of p53 protein and flow cytometric DNA analysis in the study of serous effusions. Acta Cytol. 41: 1719-25.
  • Luca, D., Luca, V., Cotor, F., Raileanu, L., 1987. Invivoand in vitro cytogenetic damage in duced by sodium nitrite. Mutat Res. 189: 333-39.
  • Munzer, R., Guigas, C., Renner, H.W., 1990. Re-examination of potassium sorbate and sodium sorbate for possible genotoxic potential. Food Chem Toxicol. 28. 397-01.
  • Meng, Z., Zhang, L., 1992. Cytogenetic damage induced in human lymphocytes by sodium bisulfite. Mutat Res. 298: 63-69.

Gelişmekte Olan Tavuk Karaciğeri Üzerine Gıda Katkı Maddesi

Year 2016, Volume: 37 Issue: 2, 85 - 98, 20.04.2016

Yücel Başimoğlu Koca , Feryal Karakahya

https://doi.org/10.17776/csj.29808

Abstract

Bu çalışmada sodyum benzoata (E211) farklı doz ve sürelerle maruz kalan tavuk embriyosu karaciğer dokusuna etkileri histopatolojik yönden araştırılmıştır. Sodyum benzoat, inkübasyonun 5. gününde üç ayrı dozda (250, 500, 1000 mg/kg) vitellusa enjekte edildi. Kontrol ve deney gruplarına ait embriyolar inkübasyonun 7. (safha 31) ve 10. günlerinde (safha 36) çıkarıldı, ağırlıkları tartıldı. Yapılan istatistiki değerlendirmeler sonucunda sodyum benzoatın doza ve süreye bağlı olarak embriyoların toplam ağırlıklarında anlamlı bir azalmaya neden olduğu belirlendi (P

Keywords

Gıda katkısı sodyum benzoat tavuk embriyo karaciğer histopatoloji

References

  • Xue, B., Wang, H.F., Xu, L.H., Sun, H.F., Liu, Y.F., Peng, S.X., Liu, KX., Guo, Z.Y.,2004. Benzoate derived DNA adduction and its decay in mice measured by AMS. Nucl Instr Meth Phys ResB. 223-224: 765-69.
  • Türkoğlu, Ş., 2007. Genotoxicity of five food preservative stested on roottips of Allium cepaL. Mutat Res. 626: 4-14.
  • Chen, Q., Huang, N., Huang, J., Chen, S., Fan, J., Li, C., Xie, F.K., 2009. Sodium benzoate exposure down regulates the expression of tyrosine hydroxylase and dopamine transporter in dopaminergic neurons in developing zebrafish. Birth Defects Res (Part B). 86: 85-91.
  • Fujitani, T., 1993. Short-term effect of sodium benzoate in F344 rats and B6C3F1 mice. Toxicol Letters. 69: 171-79.
  • Sinha, R., D’Souza, D., 2010. Liver cell damage caused due to sodium benzoate toxicity in mice. Int J Biotech Biochem. 6(4): 549-54.
  • FDA (Food and Drug Administration): Food ingredients and packaging terms. Food Additive. See: Avaliable from: http://www.fda.gov/Food/FoodIngredientsPackaging/ucm064228.htm– Accessed:20.05.2010
  • Food and Organization (FAO): Sodium benzoate. http://www.fao.org/ag/agn/jecfa- additives/specs/Monograph1/Additive-393.pdf – Accessed: 20.05.2010.
  • Srour R: Benzoic acid and derivatives. Aromatic intermediat esandderivative. Paris, A.IV.1– A.IV.17, 1998.
  • RxMED: The website for family, physicians and you. The comprehensive recource for physicians, drug and illness information. See: Avaliable from: http://www.rxmed.com/b.main/b2.pharmaceutical/b2.1.monographs/CPS– Accessed: 20.11.2012. [10]. [10] EWG’s Skin Deep Cosmetics Database: Avaliable at:http://www.cosmeticsdatabase.com– Accessed: 20.05.2010.
  • Baldwin, E.A., Nisperos-Carriedo, M.O., Baker, R.A., 1995. Use of edible coatings to preserve quality of lightly (and slightly) processed products. Crit Rev Food Sci Nutr,35(6): 509-24.
  • World Health Organization (WHO):World health organization coincises international assessment document 26, benzoic acid and sodium benzoate. Genova, Switzerland. See: Avaliable from:http://www.who.int/ipcs/publications/cicad/cicad26_rev_1.pdf– Accessed: 22.09.2011.
  • Dawson, D.A., Schultz, T.W., Hunter, R.S., 1996. Developmental toxicity of carboxylic acids to Xenopus embryos: A quantitative structure activity relationship and computer- automated structure evaluation. Teratog Carcinog Mutage.16: 109-124.
  • Tsay, H.J., Wang, Y.H., Chen, W.L., Huang, M.Y., Chen, Y.H., 2007. Treatment with sodium benzoate leads to malformation of zebra fish larvae. Neurotoxicol Teratol.29: 562- 69.
  • Onodera, H., Ogiu, T., Matsuoka, C., Furuta, K., Takeuchi, M., Oono, Y., Kubota, T., Miyahara, M., Maekawa, A., Odashima, S., 1978. Studies on effects of sodium benzoate on fetuses and offspring of wistar rats. Eisei Shikenjo Hokoku.96: 47-55.
  • Concise International Chemical Assessment Document (CICAD)(2000). Benzoic acid and sodium benzoate. Document: 26. See: Available from: http://www.inchem.org/documents/cicads/cicads/cicad26.htm– Accessed:24.10.2010.
  • Hamburger, V., Hamilton, H.L., 1992. A series of normal stages in the development of chick embryo. Dev Dynam.195: 231-72.
  • Bancroft, J.D., Cook, H.C., 1994. Manual of Histological Techniques and Their Diagnostic Application. 457 p, New York, Churchill Livingstone.
  • Minor, J.L., Becker, J.A., 1971. A comparison of the teratogenic properties of sodium salicylate, sodium benzoate and phenol. Toxicol Appl Pharm. 19: 373.
  • Kaboğlu, A., Aktaç, T,A., 2002. Study of the effects of the sodium benzoate on the mouse liver. Biol Brat. 57(3): 373-80.
  • Çalışır Erden, Z., Çalışkan, D., 2003. Gıda katkı maddeleri ve insan sağlığı üzerine etkileri. Ankara Ecz Fak Derg. 32(3): 193-206.
  • Lahijani, H.A.K., Rahimpour-Bonab, H., Tavakoli, V., Hosseindoost, M., 2009. Evidence for late holocene highstands in central Guilan. Quatern Int. 197: 55-71.
  • De Robberties, E.D.P., De Robberties, E.M.F., 1980. Cell and molecular biology. INFO- MED. Int. Ed.
  • El-Elaimy, I.A., Sakr, S.A., El-Sadeny, M.M., Gabr, S.A., 1993. Electron microscopic study of the liver of Tilapia exposed to neopybuthrin. Bull Environ Contam Toxicol. 50: 682-88.
  • Nair, B., 2001. Cosmetic ingredient review expert panel “Final report on the safety assessment of benzyl alcohol, benzoic acid and sodium benzoate”. Int J Toxicol. 3: 23-50.
  • Zengin, N., Yüzbaşıoğlu, D., Ünal, F., Yılmaz, S., Aksoy, H., 2011. The evaluation of the genotoxicity of two food preservatives: Sodium benzoateand potassium benzoate. Food Chem Toxicol. 49: 763-69.
  • Sasaki, Y.F., Kawaguchi, S., Kamaya, A., Ohshita, M., Kabasawa, K., Iwama, K., Taniquchi, K., Tsuda, S., 2002. The coment assay with 8 mouse organs: result with 39 currently used food additives. Mutat Res. 519: 103-19.
  • Sarıkaya, R., Solak, K., 2003. Genotoxicity of benzoic asit studied in the Drosophila melanogaster somatic mutation and recombination test (SMART).Gazi Eğt Fak Derg. 23(3): 19-32.
  • Mpountoukas, P., Vantarakis, A., Sivridis, E., Lialiaris, T., 2008. Cytogenetic study in cultured human lymphocytes treated with three commonly used preservatives. Food Chem Toxicol,46(7): 2390-93.
  • Yılmaz, S., Ünal, F., Yüzbaşıoğlu, D., 2009. The in vitro genotoxicity of benzoic acid in
  • human peripheral blood lymphocytes. Cytotechn. 60: 55-61.
  • Darnell, J., Lodish, H., Baltimore, D., 1986. Molecular cell biology. Scientific American Books New York, p269-369.
  • Egan, M.J., Crocker, J., 1992. Nucleolar organizer regions in pathology. British Journal of Cancer.65: 1-7.
  • Giri, D.D., Nottingham, J.F., Lawry, J., Dundas, S.A., Underwood, J.C.E., 1989. Silver binding nucleolar organiser regions (agnors) in benign and malignant breast lesions: correlations with ploidy and growth phase by DNA flow cytometry. J Path. 157: 307-13.
  • Chern, J.H., Lee, Y.C., Yang, M.H., Chang, S.C., Perng, R.P., 1997. Usefulness of argyrophilic nucleolar organiser regions score to differentiate suspicious malignancy in pulmonary cytology. Chest. 111: 1591-96.
  • Lee, J.S., Lee, M.S., Park, C.S., Juhng, S.W., 1997. Diagnostic value of p53 protein and flow cytometric DNA analysis in the study of serous effusions. Acta Cytol. 41: 1719-25.
  • Luca, D., Luca, V., Cotor, F., Raileanu, L., 1987. Invivoand in vitro cytogenetic damage in duced by sodium nitrite. Mutat Res. 189: 333-39.
  • Munzer, R., Guigas, C., Renner, H.W., 1990. Re-examination of potassium sorbate and sodium sorbate for possible genotoxic potential. Food Chem Toxicol. 28. 397-01.
  • Meng, Z., Zhang, L., 1992. Cytogenetic damage induced in human lymphocytes by sodium bisulfite. Mutat Res. 298: 63-69.
There are 38 citations in total.

Details

Journal Section Natural Sciences Research Article
Authors

Yücel Başimoğlu Koca

Feryal Karakahya This is me

Publication Date April 20, 2016
Published in Issue Year 2016 Volume: 37 Issue: 2

Cite

APA Koca, Y. B., & Karakahya, F. (2016). Effects of the Food Additive Sodium Benzoate on Developing Chicken Liver. Cumhuriyet Üniversitesi Fen Edebiyat Fakültesi Fen Bilimleri Dergisi, 37(2), 85-98. https://doi.org/10.17776/csj.29808
AMA Koca YB, Karakahya F. Effects of the Food Additive Sodium Benzoate on Developing Chicken Liver. Cumhuriyet Üniversitesi Fen Edebiyat Fakültesi Fen Bilimleri Dergisi. April 2016;37(2):85-98. doi:10.17776/csj.29808
Chicago Koca, Yücel Başimoğlu, and Feryal Karakahya. “Effects of the Food Additive Sodium Benzoate on Developing Chicken Liver”. Cumhuriyet Üniversitesi Fen Edebiyat Fakültesi Fen Bilimleri Dergisi 37, no. 2 (April 2016): 85-98. https://doi.org/10.17776/csj.29808.
EndNote Koca YB, Karakahya F (April 1, 2016) Effects of the Food Additive Sodium Benzoate on Developing Chicken Liver. Cumhuriyet Üniversitesi Fen Edebiyat Fakültesi Fen Bilimleri Dergisi 37 2 85–98.
IEEE Y. B. Koca and F. Karakahya, “Effects of the Food Additive Sodium Benzoate on Developing Chicken Liver”, Cumhuriyet Üniversitesi Fen Edebiyat Fakültesi Fen Bilimleri Dergisi, vol. 37, no. 2, pp. 85–98, 2016, doi: 10.17776/csj.29808.
ISNAD Koca, Yücel Başimoğlu - Karakahya, Feryal. “Effects of the Food Additive Sodium Benzoate on Developing Chicken Liver”. Cumhuriyet Üniversitesi Fen Edebiyat Fakültesi Fen Bilimleri Dergisi 37/2 (April 2016), 85-98. https://doi.org/10.17776/csj.29808.
JAMA Koca YB, Karakahya F. Effects of the Food Additive Sodium Benzoate on Developing Chicken Liver. Cumhuriyet Üniversitesi Fen Edebiyat Fakültesi Fen Bilimleri Dergisi. 2016;37:85–98.
MLA Koca, Yücel Başimoğlu and Feryal Karakahya. “Effects of the Food Additive Sodium Benzoate on Developing Chicken Liver”. Cumhuriyet Üniversitesi Fen Edebiyat Fakültesi Fen Bilimleri Dergisi, vol. 37, no. 2, 2016, pp. 85-98, doi:10.17776/csj.29808.
Vancouver Koca YB, Karakahya F. Effects of the Food Additive Sodium Benzoate on Developing Chicken Liver. Cumhuriyet Üniversitesi Fen Edebiyat Fakültesi Fen Bilimleri Dergisi. 2016;37(2):85-98.