Disinfection Effect of Gaseous Ozone on Candida albicans and Enterococcus faecalis: A Randomized Clinical Trial in Infected Primary Molars

Year 2023, Volume: 33 Issue: 4 - 2023, 33:4, 219 - 225, 10.10.2023

Efsun Songür Merve Aksoy Berrin Özçelik Firdevs Tulga Öz

https://doi.org/10.5152/CRDS.2023.22094

Cited By: 1

Abstract

Objective: The current clinical randomized study’s primary purpose was to investigate the antimicrobial efficiency of gaseous ozone on primary teeth root canals.
Methods: The included teeth were randomly divided into three groups, and in each group, the root canal therapies of 12 primary teeth were done. In group 1, 2.5% sodium hypochlorite was applied as an irrigation agent. In group 2, 0.9% sterile saline solution was combined with 80 sec- onds OzonyTronX® application. In group 3, 2.5% sodium hypochlorite was applied as an intracanal medicament in combination with 80 seconds OzonyTronX®. Microbial analyses assessed the colo- nization of Candida albicans and Enterococcus faecalis before and after the procedure.
Results: Thirty-six primary molars of 36 completely healthy patients (aged 5-10) were included in the study. The results revealed that the percentage decrease in the number of C. albicans was higher in group 3 (99.3%). This decrease was statistically higher than group 1 (96.1%) and group 2 (94.2%). Group 1 showed better results in eliminating the viable C. albicans versus group 2
(P = .033). The decrease in the number of E. faecalis was higher in group 3 (99.4%) compared to group 1 (99.1%) and group 2 (98.7%) (P = .006). Although the percentage of the unviable microor- ganisms was higher in group 1, there was no statistical difference versus group 2 (P > .05).
Conclusion: Using gaseous ozone alone or in combination with sodium hypochlorite may enhance the success of the antimicrobial stage of primary root canal therapies. It could be an alternative to toxic chemical agents such as highly concentrated sodium hypochlorite.
Keywords: Dentistry, disinfection agents, ozone, pediatric dentistry, primary teeth, root canal treatment

ÖZ
Amaç: Bu çalışmada ozon gazının süt dişi kök kanalları üzerindeki antimikrobiyal etkinliğinin ince- lenmesi amaçlanmıştır.
Yöntemler: Dahil edilen dişler rastgele three gruba ayrıldı ve her grupta 12 süt dişinin kanal teda- visi yapıldı. Grup 1’de irrigasyon ajanı olarak %2,5 NaOCl uygulandı. Grup 2’de %0,9 steril salin solüsyonu 80 saniye OzonyTronX® uygulaması ile kombine edildi. Grup 3’te %2,5 NaOCl kanal içi ilaç olarak 80 saniye OzonyTronX® ile kombinasyon halinde uygulandı. Mikrobiyal analizler ile işlemden önce ve sonra C. albicans ve E. faecalis’in kolonizasyonu değerlendirdi.
Bulgular: Tamamen sağlıklı 36 hastanın (5-10 yaş arası) 36 süt azı dişi çalışmaya dahil edildi. Sonuçlar, Candida albicans sayısındaki azalmanın Grup 3’te (%99,3) daha yüksek olduğunu ortaya koydu. Bu azalma Grup 1 (%96,1) ve Grup 2’ye (%94,2) göre istatistiksel olarak daha yüksekti. Grup
1’in Candida Albicans üzerindeki antimikrobiyal etkisi Grup 2’ye kıyasla daha yüksek bulundu (P = ,033). Enterococcus faecalis sayısındaki azalma Grup 3’te (%99,4) Grup 1 (%99,1) ve Grup 2’ye (%98,7) göre daha fazlaydı (P = ,006). Canlı olmayan mikroorganizmaların yüzdesi Grup 1’de daha yüksek olmasına rağmen, Grup 2’ye göre istatistiksel olarak anlamlı bir fark tespit edilemedi (P > ,05).
Sonuç: Ozone gazının yalnız ya da sodyum hipoklorit ile kombine kullanımının süt dişi kanal tedavilerinin antimikrobiyal etkinliğini arttırabileceği ve bu uygulamanın toksik kimyasal kanal içi dezenfektanlara alternatif olabileceği düşünülmektedir.
Anahtar Kelimeler: Diş hekimliği, dezenfekte edici ajanlar, ozon, çocuk diş hekimliği, süt dişleri, kök kanal tedavisi

Keywords

Dentistry, disinfection agents, ozone, pediatric dentistry, primary teeth

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