Evaluation of Platelet Rich Fibrin Content Among Individuals With Different Blood Groups

Year 2024, Volume: 34 Issue: 3, 179 - 184, 28.07.2024

G.alev Öztaş Şahiner Taner Arabaci Didem Özkal Eminoğlu Ramazan Kalin

https://doi.org/10.5152/CRDS.2023.2261104

Abstract

Objectives: Platelet Rich Fibrin (PRF), obtained by centrifuging the peripheral blood sample of the person is an autogenous material and can be affected by systemic and genetic characteristics of the belonging person. This study was undertaken for evaluating the effect of blood group types and the diverse preparation protocols on growth factors and cytokines contained in the PRF.
Methods: In total, 192 blood samples were taken from 64 donors, including 16 individuals from each blood group of 4, and from each individual 3 samples were taken for obtaining PRF, titanium platelet‐rich fibrin (T-PRF), and CGF (concentrated growth factors). The platelet-derived growth factor (PDGF), fibroblast growth factor (FGF), vascular endothelial growth factor (VEGF), angiogenin (Ang), osteocalcin, and osteonectin contents of the PRF were measured with ELISA set.
Results: When the relationship between blood groups was examined, the results indicated that there were no statistically significant differences (p>0.05). On the other hand, growth factor content was evaluated to be higher in T-PRF than PRF and CGF (p<0.05).
Conclusion: These data clearly demonstrate that PRF, T-PRF and CGF preparations contain significant amounts of growth factors capable of stimulating wound healing. However, this content is independent of blood group distribution and depends on fibrin acquisition protocols.
Keywords; Blood group antigens, Enzyme-linked immunosorbent assay, Fibroblast growth factors, Osteocalcin, platelet-rich fibrin,

Keywords

Blood group antigens, Enzyme-linked immunosorbent assay, Fibroblast growth factors, Osteocalcin, platelet-rich fibrin

References

• 1. Cochran DL, Wozney JM. Biological mediators for periodontal regeneration. Periodontol 2000. Feb 1999;19:40-58. doi:10.1111/j.1600-0757.1999.tb00146.x
• 2. Dangaria SJ, Ito Y, Walker C, Druzinsky R, Luan X, Diekwisch TG. Extracellular matrix-mediated differentiation of periodontal progenitor cells. Differentiation. 2009;78(2-3):79-90. doi:10.1016/j.diff.2009.03.005
• 3. Whitman DH, Berry RL, Green DM. Platelet gel: an autologous alternative to fibrin glue with applications in oral and maxillofacial surgery. J Oral Maxillofac Surg. 1997;55(11):1294-9. doi:10.1016/s0278-2391(97)90187-7
• 4. Korkmaz B, Kesim B. Konsantre büyüme faktörünün hücre ve dokular üzerindeki etkileri ve rejeneratif tedavide uygulama alanları. Yeditepe J Dent. 2022;18(1):71-80. doi: 10.5505/yeditepe.2022.29981
• 5. Dohan Ehrenfest DM, Rasmusson L, Albrektsson T. Classification of platelet concentrates: from pure platelet-rich plasma (P-PRP) to leucocyte- and platelet-rich fibrin (L-PRF). Trends Biotechnol. 2009;27(3):158-67. doi:10.1016/j.tibtech.2008.11.009
• 6. Dohan DM, Choukroun J, Diss A, et al. Platelet-rich fibrin (PRF): a second-generation platelet concentrate. Part II: platelet-related biologic features. Oral Surg Oral Med Oral Pathol Oral Radiol Endod. 2006;101(3):e45-50. doi:10.1016/j.tripleo.2005.07.009
• 7. Kang YH, Jeon SH, Park JY, et al. Platelet-rich fibrin is a Bioscaffold and reservoir of growth factors for tissue regeneration. Tissue Eng Part A. 2011;17(3-4):349-59. doi:10.1089/ten.TEA.2010.0327
• 8. Enawgaw B, Aynalem M, Melku M. Distribution of ABO and Rh-D Blood Group Antigens Among Blood Donors in the Amhara Regional State, Ethiopia. J Blood Med. 2022;13:97-104. doi: 10.2147/JBM.S356425
• 9. Allan, TM. Hirszfeld and the ABO blood groups. Br J Prev Soc Med. 1963;17(4):166-171. doi: 10.1136/jech.17.4.166
• 10. Jesch U, Endler PC, Walkerdorfer B, Spranger H. ABO Blood group. Related investigations and their association with defined pathologies. Sci World J. 2007;10(7):1151-4. doi: 10.1100/tsw.2007.133
• 11. Wu O, Bayoumi N, Vickers MA, Clark PABO. ABO (H) blood groups and vascular disease: a systematic review and meta‐analysis. J Thromb Haemost. 2008);6(1):62-69. doi: 10.1111/j.1538-7836.2007.02818.x
• 12. Demir T, Tezel A, Orbak R, Eltas A, Kara C, Kavrut F. The effect of ABO blood types on periodontal status. Eur J Dent. 2007;1(03):139-143. doi: 10.1055/s-0039-1698329
• 13. Bussel JB, Kunicki TJ, Michelson AD. Platelets: New Understanding of Platelet Glycoproteins and Their Role in Disease. Hematology. 2000;(1):222-240. doi:10.1182/asheducation.V2000.1.222.222
• 14. Barbalic M, Dupuis J, Dehghan A, et al. Large-scale genomic studies reveal central role of ABO in sP-selectin and sICAM-1 levels. Hum Mol Genet. 2010;19(9):1863-72. doi:10.1093/hmg/ddq061
• 15. Mohanty D, Ghosh K, Marwaha N, Kaur S, Chauhan A, Das K. Major blood group antigens-a determinant of factor VIII levels in blood? Thromb Haemostas. 1984;51(03):414-414. doi: 10.1055/s-0038-1661121
• 16. Ghanaati S, Booms P, Orlowska A, et al. Advanced platelet-rich fibrin: a new concept for cell-based tissue engineering by means of inflammatory cells. J Oral Implantol. 2014;40(6):679-89. doi:10.1563/aaid-joi-D-14-00138
• 17. Chatterjee A, Pradeep AR, Garg V, Yajamanya S, Ali MM, Priya VS. Treatment of periodontal intrabony defects using autologous platelet-rich fibrin and titanium platelet-rich fibrin: a randomized, clinical, comparative study. J Investig Clin Dent. 2017;8(3):e12231. doi:10.1111/jicd.12231
• 18. Kim T-H, Kim S-H, Sándor GK, Kim Y-D. Comparison of platelet-rich plasma (PRP), platelet-rich fibrin (PRF), and concentrated growth factor (CGF) in rabbit-skull defect healing. Arch Oral Biol. 2014;59(5):550-558. doi: 10.1016/j.archoralbio.2014.02.004
• 19. Kobayashi E, Fluckiger L, Fujioka-Kobayashi M, et al. Comparative release of growth factors from PRP, PRF, and advanced-PRF. Clin Oral Investig. 2016;20(9):2353-2360. doi:10.1007/s00784-016-1719-1
• 20. Kumar RV, Shubhashini N. Platelet rich fibrin: a new paradigm in periodontal regeneration. Cell Tissue Bank. 2013;14(3):453-63. doi:10.1007/s10561-012-9349-6
• 21. Masuki H, Okudera T, Watanebe T, et al. Growth factor and pro-inflammatory cytokine contents in platelet-rich plasma (PRP), plasma rich in growth factors (PRGF), advanced platelet-rich fibrin (A-PRF), and concentrated growth factors (CGF). Int J Implant Dent. 2016;2(1):19. doi:10.1186/s40729-016-0052-4
• 22. Dohan Ehrenfest DM, Bielecki T, Jimbo R, et al. Do the fibrin architecture and leukocyte content influence the growth factor release of platelet concentrates? An evidence-based answer comparing a pure platelet-rich plasma (P-PRP) gel and a leukocyte- and platelet-rich fibrin (L-PRF). Curr Pharm Biotechnol. 2012;13(7):1145-52. doi:10.2174/138920112800624382
• 23. Su CY, Kuo YP, Tseng YH, Su CH, Burnouf T. In vitro release of growth factors from platelet-rich fibrin (PRF): a proposal to optimize the clinical applications of PRF. Oral Surg Oral Med Oral Pathol Oral Radiol Endod. 2009;108(1):56-61. doi:10.1016/j.tripleo.2009.02.004
• 24. Clipet F, Tricot S, Alno N, et al. In vitro effects of Choukroun's platelet-rich fibrin conditioned medium on 3 different cell lines implicated in dental implantology. Implant Dent. 2012;21(1):51-6. doi:10.1097/ID.0b013e31822b9cb4
• 25. Dereka XE, Markopoulou CE, Vrotsos IA. Role of growth factors on periodontal repair. Growth Factors. 2006;24(4):260-267. doi:10.1080/08977190601060990
• 26. Hadi RS, Kusumah I, Sandra Y. Pengaruh Platelet-Rich Plasma (PRP) Terhadap Proliferasi dan Viabilitas Human Dermal Fibroblast (HDF) dalam Konsentrasi Glukosa Tinggi. Jurnal Biologi Indonesia. 2019;15(2):213-217 doi:10.14203/jbi.v15i2.3815
• 27. Selezneva IA, Gilmiyarova FN, Borodina IA, Ereshchenko AA, Gilmiyarov EM, Kartashov VV. [capital ES, Cyrilliclinicmolecular indicators of inflammatory destructive damage of the oral cavity in periodontitis in persons with various group accessories of blood.]. Klin Lab Diagn. 2020;65(2):100-105. doi:10.18821/0869-2084-2020-65-2-100-105
• 28. Arabaci T, Albayrak M. Titanium-prepared platelet-rich fibrin provides advantages on periodontal healing: A randomized split-mouth clinical study. J Periodontol. 2018;89(3):255-264. doi:10.1002/JPER.17-0294
• 29. Dohan Ehrenfest DM, Pinto NR, Pereda A, et al. The impact of the centrifuge characteristics and centrifugation protocols on the cells, growth factors, and fibrin architecture of a leukocyte- and platelet-rich fibrin (L-PRF) clot and membrane. Platelets. 2018;29(2):171-184. doi:10.1080/09537104.2017.1293812
• 30. Choukroun J, Ghanaati S. Reduction of relative centrifugation force within injectable platelet-rich-fibrin (PRF) concentrates advances patients' own inflammatory cells, platelets and growth factors: the first introduction to the low speed centrifugation concept. Eur J Trauma Emerg Surg. 2018;44(1):87-95. doi:10.1007/s00068-017-0767-9