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Polywave LED Işığı, Diyot Lazer ve Er, Cr:YSGG Lazer Kullanılarak Aktivasyon ile Ağartma Tedavisinin Etkinliğinin Karşılaştırılması: Bir İn Vitro Çalışma

Year 2025, Volume: 35 Issue: 4, 312 - 317, 21.10.2025
https://doi.org/10.17567/currresdentsci.1470751

Abstract

Amaç: Bu in vitro çalışmanın amacı, lazer ve polywave LED ışığı ile aktive edilen non-vital beyazlatma tedavilerinin etkinliğini değerlendirmektir.
Yöntemler: Kırk sekiz adet çekilmiş maksiller kesici diş koyun kanı kullanılarak yapay olarak renklendirilmiş ve daha sonra renklenen dişlerin ilk renk değerleri spektrofotometre ile ölçülmüş ve kaydedilmiştir. Dişler rastgele dört gruba ayrılmıştır (n = 12): Grup 1'e (kontrol) %35 hidrojen peroksit (H₂O₂) ile 30 dakikalık vital olmayan beyazlatma uygulanmıştır; Grup 2'ye diyot lazer (940 nm) ile %35 H₂O₂ uygulanmıştır; Grup 3'e Er,CrYSGG lazer (2780 nm) ile %35 H₂O₂; ve Grup 4'e polywave LED ışık (480 nm) ile %35 H₂O₂ verilmiştir. Renk değişiminin (ΔE00) spektrofotometrik değerlendirmesi tedavi sonrası 1, 3 ve 7. günlerde yapılmıştır.
Bulgular: Gruplar arası karşılaştırmalar, tüm zaman noktalarında Grup 2 (H₂O₂ + diyot lazer) ve Grup 3 (H₂O₂ + Er,CrYSGG lazer) ile karşılaştırıldığında Grup 1 (kontrol) ve Grup 4'te (H₂O₂ + polidalga LED) önemli ölçüde daha düşük ΔE00 değerleri göstermiştir (P <,05). Grup 2 ve 3 arasında hiçbir zaman noktasında ΔE00 açısından anlamlı bir fark gözlenmemiştir (P > ,05).
Sonuç: Hem diyot lazer hem de Er,CrYSGG lazer non-vital beyazlatmanın etkinliğini önemli ölçüde artırmıştır. Polywave LED ışığı yeterli ağartma sonuçları elde etmesine rağmen, aktivasyonu lazer tedavilerinden daha az etkili olmuştur.
Anahtar Kelimeler: Renk değişimi, lazer, LED, spektrofotometrik analiz, diş beyazlatma, nonvital

References

  • 1. Zimmerli B, Jeger F, Lussi A. Bleaching of nonvital teeth. A clinically relevant literature review. Schweiz Monatsschr Zahnmed. 2010;120(4):306-320.
  • 2. Badole GP, Warhadpande MM, Bahadure RN, Badole SG. Aesthetic Rehabilitation of Discoloured Nonvital Anterior tooth with Carbamide Peroxide Bleaching: Case Series. J Clin Diagn Res. 2013;7(12):3073-3076.
  • 3. Lim MY, Lum SO, Poh RS, Lee GP, Lim KC. An in vitro comparison of the bleaching efficacy of 35% carbamide peroxide with established intracoronal bleaching agents. Int Endod J. 2004;37(7):483-488.
  • 4. White D, Kozak K, Zoladz J, Duschner H, Götz H. Peroxide interactions with hard tissues: effects on surface hardness and surface/subsurface ultrastructural properties. Compend Contin Educ Dent. 2002;23(1A):42-50.
  • 5. Barakah R, Alwakeel R. Non-vital Endo Treated Tooth Bleaching with Sodium Perborate. Curr Health Sci J. 2019;45(3):329-332.
  • 6. Papadopoulos A, Dionysopoulos D, Strakas D, Koumpia E, Tolidis K. Spectrophotometric evaluation of the effectiveness of Er,Cr:YSGG laser-assisted intracoronal tooth bleaching treatment using different power settings. Photodiagnosis Photodyn Ther. 2021;34:102272.
  • 7. Kossatz S, Dalanhol AP, Cunha T, Loguercio A, Reis A. Effect of light activation on tooth sensitivity after in-office bleaching. Oper Dent. 2011;36(3):251-257.
  • 8. Hahn P, Schondelmaier N, Wolkewitz M, Altenburger MJ, Polydorou O. Efficacy of tooth bleaching with and without light activation and its effect on the pulp temperature: an in vitro study. Odontology. 2013;101(1):67-74.
  • 9. De Moor RJ, Verheyen J, Verheyen P, et al. Laser teeth bleaching: evaluation of eventual side effects on enamel and the pulp and the efficiency in vitro and in vivo. Sci World J. 2015;2015:835405.
  • 10. Buchalla W, Attin T. External bleaching therapy with activation by heat, light or laser--a systematic review. Dent Mater. 2007;23(5):586-596.
  • 11. De Moor RJ, Verheyen J, Diachuk A, et al. Insight in the chemistry of laser-activated dental bleaching. Sci World J. 2015;2015:650492.
  • 12. Calderini A, Sciara S, Semeria C, Pantaleo G, Polizzi E. Comparative clinical and psychosocial benefits of tooth bleaching: different light activation of a 38% peroxide gel in a preliminary case-control study. Clin Case Rep. 2016;4(8):728-735.
  • 13. Mondelli R, Rizzante F, Rosa ER, Borges A, Furuse AY, Bombonatti J. Effectiveness of LED/Laser Irradiation on In-Office Dental Bleaching after Three Years. Oper Dent. 2018;43(1):31-37.
  • 14. Najeeb S KZ, Zafar MS, Ajlal S. Applications of Light Amplification by Stimulated Emission of Radiation (Lasers) for Restorative Dentistry. Med Princ Pract. 2016;25(3):201-211.
  • 15. Dostalova T, Jelinkova H. Lasers in dentistry: overview and perspectives. Photomed Laser Surg. 2013;31(4):147-149.
  • 16. Diaci J, Filipic C, Perhavec T, Lukac M. Influence of water absorption shift on ablation speed of Er: YAG and Er, Cr: YSGG dental lasers. J Laser Heal Acad. 2013:17-22.
  • 17. Joiner A. The bleaching of teeth: a review of the literature. J Dent. 2006;34(7):412-419.
  • 18. Kurachi C, Tuboy AM, Magalhães DV, Bagnato VS. Hardness evaluation of a dental composite polymerized with experimental LED-based devices. Dent Mater. 2001;17(4):309-315.
  • 19. Arslan H, Akcay M, Yasa B, Hatirli H, Saygili G. Bleaching effect of activation of hydrogen peroxide using photon-initiated photoacoustic streaming technique. Clin Oral Investig. 2015;19(2):253-259.
  • 20. Freccia WF, Peters DD. A technique for staining extracted teeth: a research and teaching aid for bleaching. J Endod. 1982;8(2):67-99.
  • 21. Wajeh AS, Jawad HA. Efficacy of sodium perborate assisted by Er: Cr; YSGG laser induced photoacoustic streaming technique for enhancing the color of internally stained teeth. Lasers Dent Sci. 2024;8(1):45.
  • 22. Özer SY, Kapısız E. Comparison of Walking-Bleaching and Photon-Initiated Photoacoustic Streaming Techniques in Tooth Color Change of Artificially Colored Teeth. Photobiomodul Photomed Laser Surg. 2021;39(5):355-361.
  • 23. Kishi A, Otsuki M, Sadr A, Ikeda M, Tagami J. Effect of light units on tooth bleaching with visible-light activating titanium dioxide photocatalyst. Dent Mater J. 2011;30(5):723-729.
  • 24. CIE Central Bureau (2001) CIE Technical Report:Improvement to Industrial Color Difference Equation –Technical Report CIE 142-2001, Central Bureau, Vienna,Austria.
  • 25. Ghinea R, Pérez MM, Herrera LJ, Rivas MJ, Yebra A, Paravina RD. Color difference thresholds in dental ceramics. J Dent. 2010;38(Suppl 2):e57-64.
  • 26. Paravina RD, Ghinea R, Herrera LJ, et al. Color difference thresholds in dentistry. J Esthet Restor Dent. 2015;27(Suppl 1):S1-9.
  • 27. Saeedi R, Omrani LR, Abbasi M, Chiniforush N, Kargar M. Effect of Three Wavelengths of Diode Laser on the Efficacy of Bleaching of Stained Teeth. Front Dent. 2019;16(6):458-464.
  • 28. Dionysopoulos D, Strakas D, Tolidis K, Tsitrou E, Koumpia E, Koliniotou-Koumpia E. Spectrophotometric analysis of the effectiveness of a novel in-office laser-assisted tooth bleaching method using Er,Cr:YSGG laser. Lasers Med Sci. 2017;32(8):1811-1818.
  • 29. Wetter NU, Barroso MC, Pelino JE. Dental bleaching efficacy with diode laser and LED irradiation: an in vitro study. Lasers Surg Med. 2004;35(4):254-258.
  • 30. Coluzzi DJ. Fundamentals of dental lasers: science and instruments. Dent Clin North Am. 2004;48(4):751-770.
  • 31. Cavalli V, Silva BGD, Berger SB, Marson FC, Tabchoury CPM, Giannini M. Decomposition Rate, pH, and Enamel Color Alteration of At-Home and In-Office Bleaching Agents. Braz Dent J. 2019;30(4):385-396.
  • 32. Gurgan S, Cakir FY, Yazici E. Different light-activated in-office bleaching systems: a clinical evaluation. Lasers Med Sci. 2010;25(6):817-822.
  • 33. Antunes E, Basting R, do Amaral F, et al. Titanium dioxide nanotubes in a hydrogen peroxide-based bleaching agent: physicochemical properties and effectiveness of dental bleaching under the influence of a poliwave led light activation. Clin Oral Investig. 2023;27(4):1745-1755.
  • 34. Ragain JJ, Brodine B, Zheng Q, Blen B, GarciaGodoy F, Zhang Y. Effects of curing lights on human gingival epithelial cell proliferation. J Am Dent Assoc. 2021;152(4):260-268.
  • 35. Dionysopoulos D, Papageorgiou S, Malletzidou L, Gerasimidou O, Tolidis K. Effect of novel charcoal-containing whitening toothpaste and mouthwash on color change and surface morphology of enamel. J Conserv Dent. 2020;23(6):624-631.

Comparison of the Effectiveness of Bleaching Treatment with Activation Using Polywave LED Light, Diode Laser and Er, Cr:YSGG Laser: An in Vitro Study

Year 2025, Volume: 35 Issue: 4, 312 - 317, 21.10.2025
https://doi.org/10.17567/currresdentsci.1470751

Abstract

Objective: This study aimed to evaluate the efficacy of non-vital bleaching treatments activated by laser systems and polywave LED light.
Methods: Forty-eight extracted maxillary incisors were artificially stained using sheep blood and then the initial colour values of the stained teeth were measured and recorded with a spectrophotometer. The teeth were randomly assigned to four groups (n = 12 per group): Group 1 (control) received 30-minute non-vital bleaching treatments with 35% hydrogen peroxide (H₂O₂); Group 2 received 35% H₂O₂ with a diode laser (940 nm); Group 3 received 35% H₂O₂ with an Er,Cr YSGG laser (2780 nm); and Group 4 received 35% H₂O₂ with polywave LED light (480 nm). Spectrophotometric evaluation of color change (ΔE00) was performed on days 1, 3, and 7 post-treatment.
Results: Intergroup comparisons showed significantly lower ΔE00 values in Group 1 (control) and Group 4 (H₂O₂ + polywave LED light) compared to Groups 2 (H₂O₂ + diode laser) and 3 (H₂O₂ + Er,CrYSGG laser) across all time points (P < .05). No significant difference in ΔE00 was observed between Groups 2 and 3 at any time point (P > .05).
Conclusion: Both the diode laser and Er,CrYSGG laser significantly enhanced the efficacy of non-vital bleaching. Although polywave LED light achieved adequate bleaching results, its activation was less effective than that of laser systems.
Keywords: Color change, laser, LED, spectrophotometric analysis, teeth bleaching, nonvital

Ethical Statement

Ethics approval for the study incl. usage of retrospective anonymized data was obtained through the Ethics Committee of Afyonkarahisar University of Health Sciences (2021/221, date: April 2, 2021).

References

  • 1. Zimmerli B, Jeger F, Lussi A. Bleaching of nonvital teeth. A clinically relevant literature review. Schweiz Monatsschr Zahnmed. 2010;120(4):306-320.
  • 2. Badole GP, Warhadpande MM, Bahadure RN, Badole SG. Aesthetic Rehabilitation of Discoloured Nonvital Anterior tooth with Carbamide Peroxide Bleaching: Case Series. J Clin Diagn Res. 2013;7(12):3073-3076.
  • 3. Lim MY, Lum SO, Poh RS, Lee GP, Lim KC. An in vitro comparison of the bleaching efficacy of 35% carbamide peroxide with established intracoronal bleaching agents. Int Endod J. 2004;37(7):483-488.
  • 4. White D, Kozak K, Zoladz J, Duschner H, Götz H. Peroxide interactions with hard tissues: effects on surface hardness and surface/subsurface ultrastructural properties. Compend Contin Educ Dent. 2002;23(1A):42-50.
  • 5. Barakah R, Alwakeel R. Non-vital Endo Treated Tooth Bleaching with Sodium Perborate. Curr Health Sci J. 2019;45(3):329-332.
  • 6. Papadopoulos A, Dionysopoulos D, Strakas D, Koumpia E, Tolidis K. Spectrophotometric evaluation of the effectiveness of Er,Cr:YSGG laser-assisted intracoronal tooth bleaching treatment using different power settings. Photodiagnosis Photodyn Ther. 2021;34:102272.
  • 7. Kossatz S, Dalanhol AP, Cunha T, Loguercio A, Reis A. Effect of light activation on tooth sensitivity after in-office bleaching. Oper Dent. 2011;36(3):251-257.
  • 8. Hahn P, Schondelmaier N, Wolkewitz M, Altenburger MJ, Polydorou O. Efficacy of tooth bleaching with and without light activation and its effect on the pulp temperature: an in vitro study. Odontology. 2013;101(1):67-74.
  • 9. De Moor RJ, Verheyen J, Verheyen P, et al. Laser teeth bleaching: evaluation of eventual side effects on enamel and the pulp and the efficiency in vitro and in vivo. Sci World J. 2015;2015:835405.
  • 10. Buchalla W, Attin T. External bleaching therapy with activation by heat, light or laser--a systematic review. Dent Mater. 2007;23(5):586-596.
  • 11. De Moor RJ, Verheyen J, Diachuk A, et al. Insight in the chemistry of laser-activated dental bleaching. Sci World J. 2015;2015:650492.
  • 12. Calderini A, Sciara S, Semeria C, Pantaleo G, Polizzi E. Comparative clinical and psychosocial benefits of tooth bleaching: different light activation of a 38% peroxide gel in a preliminary case-control study. Clin Case Rep. 2016;4(8):728-735.
  • 13. Mondelli R, Rizzante F, Rosa ER, Borges A, Furuse AY, Bombonatti J. Effectiveness of LED/Laser Irradiation on In-Office Dental Bleaching after Three Years. Oper Dent. 2018;43(1):31-37.
  • 14. Najeeb S KZ, Zafar MS, Ajlal S. Applications of Light Amplification by Stimulated Emission of Radiation (Lasers) for Restorative Dentistry. Med Princ Pract. 2016;25(3):201-211.
  • 15. Dostalova T, Jelinkova H. Lasers in dentistry: overview and perspectives. Photomed Laser Surg. 2013;31(4):147-149.
  • 16. Diaci J, Filipic C, Perhavec T, Lukac M. Influence of water absorption shift on ablation speed of Er: YAG and Er, Cr: YSGG dental lasers. J Laser Heal Acad. 2013:17-22.
  • 17. Joiner A. The bleaching of teeth: a review of the literature. J Dent. 2006;34(7):412-419.
  • 18. Kurachi C, Tuboy AM, Magalhães DV, Bagnato VS. Hardness evaluation of a dental composite polymerized with experimental LED-based devices. Dent Mater. 2001;17(4):309-315.
  • 19. Arslan H, Akcay M, Yasa B, Hatirli H, Saygili G. Bleaching effect of activation of hydrogen peroxide using photon-initiated photoacoustic streaming technique. Clin Oral Investig. 2015;19(2):253-259.
  • 20. Freccia WF, Peters DD. A technique for staining extracted teeth: a research and teaching aid for bleaching. J Endod. 1982;8(2):67-99.
  • 21. Wajeh AS, Jawad HA. Efficacy of sodium perborate assisted by Er: Cr; YSGG laser induced photoacoustic streaming technique for enhancing the color of internally stained teeth. Lasers Dent Sci. 2024;8(1):45.
  • 22. Özer SY, Kapısız E. Comparison of Walking-Bleaching and Photon-Initiated Photoacoustic Streaming Techniques in Tooth Color Change of Artificially Colored Teeth. Photobiomodul Photomed Laser Surg. 2021;39(5):355-361.
  • 23. Kishi A, Otsuki M, Sadr A, Ikeda M, Tagami J. Effect of light units on tooth bleaching with visible-light activating titanium dioxide photocatalyst. Dent Mater J. 2011;30(5):723-729.
  • 24. CIE Central Bureau (2001) CIE Technical Report:Improvement to Industrial Color Difference Equation –Technical Report CIE 142-2001, Central Bureau, Vienna,Austria.
  • 25. Ghinea R, Pérez MM, Herrera LJ, Rivas MJ, Yebra A, Paravina RD. Color difference thresholds in dental ceramics. J Dent. 2010;38(Suppl 2):e57-64.
  • 26. Paravina RD, Ghinea R, Herrera LJ, et al. Color difference thresholds in dentistry. J Esthet Restor Dent. 2015;27(Suppl 1):S1-9.
  • 27. Saeedi R, Omrani LR, Abbasi M, Chiniforush N, Kargar M. Effect of Three Wavelengths of Diode Laser on the Efficacy of Bleaching of Stained Teeth. Front Dent. 2019;16(6):458-464.
  • 28. Dionysopoulos D, Strakas D, Tolidis K, Tsitrou E, Koumpia E, Koliniotou-Koumpia E. Spectrophotometric analysis of the effectiveness of a novel in-office laser-assisted tooth bleaching method using Er,Cr:YSGG laser. Lasers Med Sci. 2017;32(8):1811-1818.
  • 29. Wetter NU, Barroso MC, Pelino JE. Dental bleaching efficacy with diode laser and LED irradiation: an in vitro study. Lasers Surg Med. 2004;35(4):254-258.
  • 30. Coluzzi DJ. Fundamentals of dental lasers: science and instruments. Dent Clin North Am. 2004;48(4):751-770.
  • 31. Cavalli V, Silva BGD, Berger SB, Marson FC, Tabchoury CPM, Giannini M. Decomposition Rate, pH, and Enamel Color Alteration of At-Home and In-Office Bleaching Agents. Braz Dent J. 2019;30(4):385-396.
  • 32. Gurgan S, Cakir FY, Yazici E. Different light-activated in-office bleaching systems: a clinical evaluation. Lasers Med Sci. 2010;25(6):817-822.
  • 33. Antunes E, Basting R, do Amaral F, et al. Titanium dioxide nanotubes in a hydrogen peroxide-based bleaching agent: physicochemical properties and effectiveness of dental bleaching under the influence of a poliwave led light activation. Clin Oral Investig. 2023;27(4):1745-1755.
  • 34. Ragain JJ, Brodine B, Zheng Q, Blen B, GarciaGodoy F, Zhang Y. Effects of curing lights on human gingival epithelial cell proliferation. J Am Dent Assoc. 2021;152(4):260-268.
  • 35. Dionysopoulos D, Papageorgiou S, Malletzidou L, Gerasimidou O, Tolidis K. Effect of novel charcoal-containing whitening toothpaste and mouthwash on color change and surface morphology of enamel. J Conserv Dent. 2020;23(6):624-631.
There are 35 citations in total.

Details

Primary Language English
Subjects Restorative Dentistry
Journal Section Research Articles
Authors

Latife Altınok Uygun 0000-0003-2593-171X

Cemile Yılmaz 0000-0002-7780-079X

Pınar Gül 0000-0003-3714-4991

Publication Date October 21, 2025
Submission Date April 19, 2024
Acceptance Date March 5, 2025
Published in Issue Year 2025 Volume: 35 Issue: 4

Cite

AMA Altınok Uygun L, Yılmaz C, Gül P. Comparison of the Effectiveness of Bleaching Treatment with Activation Using Polywave LED Light, Diode Laser and Er, Cr:YSGG Laser: An in Vitro Study. Current Research in Dental Sciences. October 2025;35(4):312-317. doi:10.17567/currresdentsci.1470751

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