Bazı abiyotik stres faktörlerine dayanımı yüksek veya duyarlı patlıcan genotiplerinde Cu, Pb, Cd ve Zn içeren sulama sularının fizyolojik ve biyokimyasal parametreler üzerine etkisi

Abstract

Çalışmada tuza tolerans düzeyleri daha önce belirlenmiş tuz ve kuraklığa tolerans (Burdur Merkez, Burdur Bucak) ve duyarlı (Kemer ve Giresun) patlıcan genotipleri kullanılmıştır. Kontrollü sera koşullarında yürütülen çalışmada, patlıcan tohumları torf ve perlit karışımı ortamında çimlendirilmiş ve ekimden 20 gün sonra fideler saksılara şaşırtılmıştır. Bitkiler 7 gün sonra içerisinde farklı dozlarda ağır metaller bulunan sulama suyu (Kontrol: 0 ppm; I. Karışım: 0.2 ppm Cu+0.01 ppm Cd+5 ppm Pb+2 ppm Zn; II. Karışım: 0.4 ppm Cu+0.02 ppm Cd+10 ppm Pb+4 ppm Zn) ile sulanmaya başlanmıştır. Bitkiler 40 gün boyunca sulandıktan sonra bu sürenin sonunda hasat edilmiş ve analizler için örnek alımı yapılmıştır. Çalışmada bitkiler, yeşil aksam ve kök yaş ağırlığı, yeşil aksam ve kök kuru ağırlığı, gövde ve kök boyu, yaprak alanı, klorofil, malondialdehit (MDA) miktarı, süperoksit dismutaz (SOD), katalaz (CAT), askorbat peroksidaz (APX) ve glutatyon redüktaz (GR) enzim aktiviteleri yönünden incelenmiştir. Bitkiler genotiplere göre değişmekle birlikte ağır metal uygulamasından olumsuz yönde etkilenmişlerdir. Ağır metal uygulamaları doz artışına bağlı olarak bitkilerin yeşil aksam ve köklerinin yaş ve kuru ağırlıklarında, kök ve gövde boylarında, yaprak alanı değerlerinde azalmaya neden olmuş, MDA ve antioksidatif enzim aktivitelerinde artış görülmüştür. Çalışma sonucunda, ağır metal uygulamalarının oluşturduğu strese karşı, tolerant Burdur Merkez ve Burdur Bucak genotipleri, hassas olan Giresun ve Kemer genotiplerine oranla daha iyi bir performans göstermiştir. Elde edilen bulgulara göre; bitkilerin tuzluluk, kuraklık ve ağır metal stresi gibi abiyotik streslere dayanım için benzer mekanizmaları kullandıkları düşünülmektedir.

Keywords

• Solanum melongena
• Genotip
• Ağır metal
• MDA
• Antioksidatif enzim

The effect of irrigation water including Cu, Pb, Cd, and Zn on the physiological and biochemical parameters in eggplant genotypes tolerant or susceptible to some abiotic stress factors

Abstract

The eggplant genotypes (Burdur Merkez, Burdur Bucak, Kemer ve Giresun) of which salt and drought tolerances were determined before by examining changes in some of the morphological and physiological characteristics were used as materials. In the studies carried out in controlled greenhouse conditions, eggplant plants were subjected to different levels heavy metal irrigation at 20 days after sowing (Control: 0 ppm; I. mixture: 0.2 ppm Cu+0.01 ppm Cd+5 ppm Pb+2 ppm Zn; II. mixture: 0.4 ppm Cu+0.02 ppm Cd+10 ppm Pb+4 ppm Zn). Young plants were harvested after forty days at heavy metal treatment and the fresh and dry shoot weight, fresh and dry root weight, shoot and root length, leaf areas, chlorophyll, malondi-aldehyde (MDA), superoxide dismutase (SOD) and glutathione reductase (GR) were determined. The eggplant plants were adversely affected by heavy metal applications. In parallel to increase the dose, heavy metal mixtures led to a reduction in values of fresh and dry weight of shoot and root, stem and root length, leaf area. MDA and antioxidative enzyme activities increased in plants irrigated with water containing a mixture of heavy metal. As a result of this study, tolerant Burdur Merkez and Burdur Bucak genotypes showed a better performance compared with the salt sensitive genotypes Giresun and Kemer. The plants are thought to use similar mechanisms for resistance to abiotic stresses such as drought and heavy metal stress.

Keywords

• Solanum melongena
• Genotype
• Heavy metal
• MDA
• Antioxidative enzymes

References

1. Ahmed, A., Hasnain, A., Akhtar, S., Hussain, A., Ullah, A., Yasin, G., Wahid, A., & Mahmood, S. (2010). Antioxidant enzymes as bio-markers for copper tolerance in safflower (Carthamus tinctorius L.). African Journal of Biotechnology, 9(33):5441-5444.
2. Bouazizi, H., Jouili, H. A., & El Ferjani, E. (2010). Copper toxicity in expanding leaves of Phaseolus vulgaris L.: Antioxidant enzyme response and nutrient element uptake. Ecotoxicology and Environmental Safety, 73(6):1304-1308.
3. Cakmak, I., & Marschner, H. (1992). Magnesium deficiency and highlight intensity enhance activities of superoxide dismutase, ascorbate peroxidase and glutathione reductase in bean leaves. Plant Physiology, 98(4):1222-1226.
4. Çakmak, I. (1994). Activity of ascorbate-dependent H202 scavenging enzymes and leaf chlorosis are enhanced in magnesium and potassium deficient leaves, but not in phosphorus deficient leaves. Journal of Experimental Botany, 45(9):1259-1266.
5. Chaoui, A., Mazhoudi, S., Ghorbal, M.H., & El Ferjani, E. (1997). Cadmium and zinc induction of lipid peroxidation and effects on antioxidant enzyme activities in bean (Phaseolus vulgaris L.). Plant Science, 127(2):139–147.
6. Daşgan, H.Y., & Koç, S. (2009). Evaluation of salt tolerance in common bean genotypes by ion regulation and searching for screening parameters. Journal of Food, Agriculture & Environment, 77:363-372.
7. Dey, S.K., Dey, J., Patra, S., & Pothal, D. (2007). Changes in the antioxidative enzyme activities and lipid peroxidation in wheat seedlings exposed to cadmium and lead stress. Brazilian Journal of Plant Physiology, 19(1):53-60.
8. Dunand, V.F., Epron, D., Sossé, A.B., & Badot, P.M. (2002). Effects of copper on growth and on photosynthesis of mature and expanding leaves in cucumber plants. Plant Science, 163(1):53-58.

9. El-Beltagi, H.S., Mohamed, A.A., & Rashed, M.M. (2010). Response of antioxidative enzymes to cadmium stress in leaves and roots of radish (Raphanus sativus L.). Notulae Scientia Biologicae, 2(4):76-82.
10. FAO (1985). Water Quality for Agriculture. FAO Irrigation and Drainage Paper. 29, Rev.1. Rome. http://www.fao.org. Erişim tarihi: 10 Mart 2014.
11. Fargašová, A. (2001). Phytotoxic effects of Cd, Zn, Pb, Cu and Fe on Sinapis alba L. seedlings and their accumulation in roots and shoots. Biologia Plantarum, 44(3):471-473.
12. Freed, R., Einensmith, S.P., Guets, S., Reicosky, D., Smail, V.W., & Wolberg, P. (1989). User’s Guide to MSTAT-C, An Analysis of Agronomic Research Experiment. Michigan State University, USA.
13. Gallego, S.M., Benavides, M.P., & Tomaro, M.L., (1996). Effect of heavy metal ion excess on sunflower leaves: Evidence for involvement of oxidative stress. Plant Science, 121 (2):151-159.
14. Gasic, K., & Korban, S.S. (2006). Heavy Metal Stress. In: Physiology and molecular biology of stress tolerance in plants. Eds: Madhava Rao, K.V., Raghavendra A.S. & Janardhan Redddy, K. pp: 219-254. Springer, Netherlands.
15. Gratão, P.L., Monteiro, C.C., Antunes, A.M., Peres, L.E.P., & Azevedo, R.A. (2008). Acquired tolerance of tomato (Lycopersicon esculentum cv. Micro-Tom) plants to cadmium-induced stress. Annals of Applied Biology, 153(3):321-333.
16. Hall, J.L. (2001). Cellular mechanisms for heavy metal detoxification and tolerance. Journal of Experimental Botany, 53(366):1-11.
17. Hegedus, A., Erdei, S., & Horvath, G. (2001). Comparative studies of H2O2 detoxifying enzymes in green and greening barley seedlings under cadmium stress. Plant Science, 160(6):1085-1093.
18. Jiang, W., & Liu, D. (2000). Effects of Pb+2 on root growth, cell division, and nucleolus of Zea mays L. Bulletin of Environmental Contamination and Toxicology, 65(6):786-793.
19. Kıran, S., Özkay, F., Ellialtıoğlu, Ş., & Kuşvuran, Ş. (2014). Tuz stresine tolerans seviyeleri belirlenmiş bazı genotiplerin kuraklık stresine tepkilerinin belirlenmesi. TAGEM Sonuç Raporu. Proje No: A-02.P-04, Ankara.
20. Kuşvuran, Ş. (2010). Kavunlarda kuraklık ve tuzluluğa toleransın fizyolojik mekanizmaları arasındaki bağlantılar. Doktora Tezi, Çukurova Üniversitesi, Adana.
21. Liu, Y., Wang, X., Zeng, G., Qu, D., Gu, J., Zhou, M., & Chai, L. (2007). Cadmium-induced oxidative stress and response of the ascorbate-glutathione cycle in Bechmeria nivea (L.) Gaud. Chemosphere, 69(1):99-107.
22. Luna, C., Seffino, L.G., Arias, C., & Taleisnik, E. (2000). Oxidative stress indicators as selection tools for salt tolerance in Chloris gayana. Plant Breeding, 119(4): 341-345.
23. Lutts, S., Kinet, J.M., & Bouharmont, J. (1996). NaCl-induced senescence in leaves of rice (Oryza sativa L.) cultivars differing in salinity resistance. Annual Botany, 78(3):389-398.
24. Mangal, J.L., & Lal, S. (1988). Salt tolerance behavior of Khorif onion variety N.53. Haryana Journal Horticultural Science, 17(1-2):78-82.
25. Manivasagaperumal, R., Vijayarengan, P., Balamurugan, S., & Thiyagarajan, G. (2011). Effect of copper on growth, dry matter yield and nutrient content of Vigna radiata (L.) Wilczek. Journal of Phytology, 3(3):53-62.
26. Morela, V.R.F., Capraru, G., Bara, I., & Artenie, V. (2007). Lead acetate effect on superoxide dismutase activity in Lactuca sativa L., Mona and Syrena cultivars. Genetics and Molecular Biology, 8 (2):115-118.
27. Moyo, D.Z., & Chimbira, C. (2009).The effect of single and mixed treatments of lead and cadmium on soil bioavailability, uptake and yield of Lactuca sativa irrigated with sewage effluent under greenhouse conditions. American-Eurasian Journal of Agriculture & Environmental Science, 6(5):526-531.
28. Prasad, K.V.S.K., Paradha, S.P., & Sharmila, P. (1999). Concerted action of antioxidant enzymes and curtailed growth under zinc toxicity in Brassica juncea. Journal Environmental and Experimental Botany, 42(1):1-10.
29. Pahlsson, A.M.B. (1989). Toxicity of heavy metals (Zn, Cu, Cd, Pb) to vascular plants: A Literature Review. Water Air Soil Pollution, 47(3):287-319.
30. Raskin, I., & Ensley, B.D. (2000). Phytoremediation of Toxic Metals: Using Plants to Clean Up The Enviroment. John Wiley and Sons, 304 pp., NewYork.
31. Saeed, A., Sohai,l M., Rashi, N., & Iqbal, N. (2013). Effects of heavy metals toxicity on the biochemical response in tomato plants grown in contaminated silt-soil. Bangladesh Journal of Scientific and Industrial Research, 48(4): 229-236.
32. Salt, D., Price, R., Pickering, I., & Raskin, I. (1995). Mechanisms of cadmium mobility and accumulation in Indian mustard. Plant Physiology, 109:1427-1433
33. Sbartai, H., Djebar, M.R., Rouabhi, R., Sbartai, I., & Berrebbah, H. (2011). Antioxidative response in tomato plants Lycopersicon esculentum L. roots and leaves to zinc. American-Eurasian Journal of Toxicological Sciences, 3(1):41-46.
34. Sharma, P., & Dubey, R.S. (2005). Lead toxicity in plants. Brazilian Journal of Plant Physiology, 17(1):35-52.
35. Sossé, B.A., Genet, P., Dunand-Vinit, F., Toussaint, L.M., Epron, D., & Badot, P.M. (2004). Effect of copper on growth in cucumber plants (Cucumis sativus) and its relationships with carbonhydrate accumulation and changes in ion contents. Plant Science, 166(5): 1213-1218.
36. Sun, T., Sha, W., & Jing, J. (2010). Effects of Zn stress on physiological characteristics of melon seedlings. Northern Horticulture, 16:51-53.
37. Taulavuori, E., Hellström, E-K., Taulavuori, K., & Laine, K. (2001). Comparison of two methods used to analyse lipid peroxidation from Vaccinium myrtillus L. during snow removal, reclamation and cold acclimation. Journal of Experimental Botany, 52(365):2375-2380.
38. Thounaojam, T. C., Panda, P., Mazumdar, P., Kumar, D., Sharma, G. D., Sahoo, L., & Panda, S. K. (2012). Excess copper induced oxidative stress and response of antioxidants in rice. Plant Physiology and Biochemistry, 53:33-39.
39. TÜİK (2014). Bitkisel üretim İstatistikleri. http://tuikrapor.tuik.gov.tr. Erişim tarihi: 6 Ağustos 2015.
40. Weck, J.E.J., & Clijsters, H.M.M. (1997). Zn phytotoxicity induces oxidative stress in primary leaves of Phaseolus vulgaris. Plant Physiology and Biochemistry, 35 (5): 405-410.
41. Vaillant, N., Monnet, F., Hitmi, A., Sallanon, H., & Coudret, A. (2005). Comparative study of responses in four Datura species to a zinc stress. Chemosphere, 59:1005-1013.
42. Van Assche, F.V., & Clijsters, H. (1990). Effects of metals on enzyme activity in plants. Plant Cell Environment, 13(3):95-206.
43. Verma, S., & Dubey, R.S. (2003). Lead toxicity induces lipid peroxidation and alters the activities of antioxidant enzymes in growing rice plants. Plant Science, 164(4):645-655.
44. Yaşar, F. (2003). Tuz stresi altındaki patlıcan genotiplerinde bazı antioksidant enzim aktivitelerinin in vitro ve in vivo olarak incelenmesi. Doktora Tezi, Yüzüncü Yıl Üniversitesi, Van.
45. Zhou, D.X., Liu, Y.F., & Liu, X.B. (2009). Effects of waterlogging stress on physiological and biochemical index in Alternant phiiloxeroides Hubei. Agricultural Sciences, 48(3):585-587.