Organik-inorganik hibrit nano çiçeklerin çemen (Trigonella foenum-graecum L.) tohum ekstresi kullanılarak sentezi ve anti-mikrobiyal özelliklerinin araştırılması

Year 2019, Volume: 36 Issue: 2, 159 - 167, 10.12.2019

Cevahir Altınkaynak , Nilay Ildız Ayşe Baldemir , Nalan Özdemir , Vedat Yılmaz İsmail Öçsoy

https://doi.org/10.16882/derim.2019.549151

Cited By: 33

https://izlik.org/JA77CE37GE

Abstract

Bu çalışmada organik-inorganik hibrit nano çiçek
sentezi için organik bileşen olarak çemen (Trigonella
foenum-graecum L.) otu tohum
ekstresi ve inorganik bileşen olarak Cu²⁺ iyonları kullanılarak
yeşil bir üretim metodu raporlanmıştır. Çemen tohum ekstresi kullanılarak
sentezlenmiş organik-inorganik hibrit nano çiçekler (TF-Cu²⁺ hNF); taramalı elektron mikroskobu (SEM), Enerji Dağılımlı Spektrometre (EDX),
X-ışını kırınımı (XRD) ve Kızılötesi spektroskopisi (FT-IR) yöntemleri
kullanılarak karakterize edilmiştir. TF-Cu²⁺ hNF’lerin elektron
mikroskop görüntüsünde morfolojisi oldukça düzgün dağılımlı ve küresel formda
olmak üzere yaklaşık 18 μm boyutunda
gözlenmiştir. TF-Cu²⁺
hNF’ler; Pseudomonas aeruginosa and Haemophilus influenza
dışında Enterococcus faecium, Enterococcus faecalis, Staphylococcus aureus,
Bacillus cereus, Salmonella typhi ve Escherichia coli türlerine
karşı 1-10 µg ml^-1 aralığında ve kullanılan antibiyotikler
ile karşılaştırıldığında yüksek düzeyde
anti-mikrobiyal özellik göstermiştir. Ancak hem TF-Cu²⁺ hNF’ler hemde serbest TF ekstresi, Candida
albicans ve Candida glabrata türlerine karşı antifungal aktivite
göstermemiştir. Yapılan bu çalışma
çemen ekstresi içeren organik-inorganik hibrit nano çiçeklerin test edilen
patojen suşlar ile gelişen mikrobiyal enfeksiyonlar için terapötik bir ajan
olarak kullanılabileceğini ve ilaç direncinin üstesinden gelme potansiyeline
sahip olduğunu ortaya koymaktadır.

Keywords

Biyomateryal , Organik - inorganik hibrid nano çiçekler , Bakır , Anti-mikrobiyal aktivite

Synthesis of organic-inorganic hybrid nanoflowers using Trigonella foenum-graecum seed extract and investigation of their anti-microbial activity

https://izlik.org/JA77CE37GE

Abstract

Herein
we report a green method for the synthesis of organic-inorganic hybrid
nanoflowers using a Trigonella
foenum-graecum L. (TF) (Fenugreek seeds) extracts as an organic part and
copper ions acting as an inorganic part. The organic-inorganic hybrid
nanoflowers using TF seed extract (TF-Cu²⁺ hNFs) were characterized
by SEM, XRD, EDX and FTIR. The morphology of the TF-Cu²⁺ hNFs was
quite spherical and monodisperse with ∼18μm size. The TF-Cu²⁺
hNF exhibited the effective anti-bacterial activity against Enterococcus
faecium, Enterococcus faecalis, Staphylococcus aureus, Bacillus cereus,
Salmonella typhi and Escherichia coli at 1-10 µg ml^-1
concentrations except against Pseudomonas aeruginosa and Haemophilus
influenza. However, both TF-Cu²⁺ hNFs and free TF extracts
showed any antifungal activities against Candida albicans or Candida
glabrata. The study revealed that TF-Cu²⁺ hNFs could be used as
a therapeutic agent for microbial infections and has the potential to overcome
drug resistance.

Keywords

Biomaterials , Organic-inorganic hybrid nanoflowers , Copper , Anti- microbial activity

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