Organik-inorganik hibrit nano çiçeklerin çemen (Trigonella foenum-graecum L.) tohum ekstresi kullanılarak sentezi ve anti-mikrobiyal özelliklerinin araştırılması

Abstract

Bu çalışmada organik-inorganik hibrit nano çiçek sentezi için organik bileşen olarak çemen (Trigonella foenum-graecum L.) otu tohum ekstresi ve inorganik bileşen olarak Cu²⁺ iyonları kullanılarak yeşil bir üretim metodu raporlanmıştır. Çemen tohum ekstresi kullanılarak sentezlenmiş organik-inorganik hibrit nano çiçekler (TF-Cu²⁺ hNF); taramalı elektron mikroskobu (SEM), Enerji Dağılımlı Spektrometre (EDX), X-ışını kırınımı (XRD) ve Kızılötesi spektroskopisi (FT-IR) yöntemleri kullanılarak karakterize edilmiştir. TF-Cu²⁺ hNF’lerin elektron mikroskop görüntüsünde morfolojisi oldukça düzgün dağılımlı ve küresel formda olmak üzere yaklaşık 18 μm boyutunda gözlenmiştir. TF-Cu²⁺ hNF’ler; Pseudomonas aeruginosa and Haemophilus influenza dışında Enterococcus faecium, Enterococcus faecalis, Staphylococcus aureus, Bacillus cereus, Salmonella typhi ve Escherichia coli türlerine karşı 1-10 µg ml^-1 aralığında ve kullanılan antibiyotikler ile karşılaştırıldığında yüksek düzeyde anti-mikrobiyal özellik göstermiştir. Ancak hem TF-Cu²⁺ hNF’ler hemde serbest TF ekstresi, Candida albicans ve Candida glabrata türlerine karşı antifungal aktivite göstermemiştir. Yapılan bu çalışma çemen ekstresi içeren organik-inorganik hibrit nano çiçeklerin test edilen patojen suşlar ile gelişen mikrobiyal enfeksiyonlar için terapötik bir ajan olarak kullanılabileceğini ve ilaç direncinin üstesinden gelme potansiyeline sahip olduğunu ortaya koymaktadır.

Keywords

• Biyomateryal,Organik - inorganik hibrid nano çiçekler,Bakır,Anti-mikrobiyal aktivite

Synthesis of organic-inorganic hybrid nanoflowers using Trigonella foenum-graecum seed extract and investigation of their anti-microbial activity

Abstract

Herein we report a green method for the synthesis of organic-inorganic hybrid nanoflowers using a Trigonella foenum-graecum L. (TF) (Fenugreek seeds) extracts as an organic part and copper ions acting as an inorganic part. The organic-inorganic hybrid nanoflowers using TF seed extract (TF-Cu²⁺ hNFs) were characterized by SEM, XRD, EDX and FTIR. The morphology of the TF-Cu²⁺ hNFs was quite spherical and monodisperse with ∼18μm size. The TF-Cu²⁺ hNF exhibited the effective anti-bacterial activity against Enterococcus faecium, Enterococcus faecalis, Staphylococcus aureus, Bacillus cereus, Salmonella typhi and Escherichia coli at 1-10 µg ml^-1 concentrations except against Pseudomonas aeruginosa and Haemophilus influenza. However, both TF-Cu²⁺ hNFs and free TF extracts showed any antifungal activities against Candida albicans or Candida glabrata. The study revealed that TF-Cu²⁺ hNFs could be used as a therapeutic agent for microbial infections and has the potential to overcome drug resistance.

Keywords

• Biomaterials,Organic-inorganic hybrid nanoflowers,Copper,Anti- microbial activity

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