Determination of Levadopa and Benserazide Active Ingredients Used in Parkinson's Treatment by Artificial Neural Networks Method

Year 2023, , 329 - 337, 15.05.2023

Ahmet Hakan Aktaş , Damla Ezgi Barikan

https://doi.org/10.21205/deufmd.2023257406

Abstract

In this study, the determinations of levadopa and benserazide active ingredients in the tablet drug sample were carried out by ultraviolet visible region spectroscopy without applying any separation process at the same time and the obtained data were evaluated by artificial neural networks (ANN) chemometric method. At the end of the study, the least squares method (PLS) was applied to compare the method used. In the study, the pure spectra of the active ingredients were obtained and then their synthetic mixtures were prepared. The ata obtained from these mixtures were evaluated with ANN and the method was applied to the tablet sample purchased from the market. The data obtained in the study were also analyzed statistically, and the root mean square (RMS) data, which is an important data group in the ANN method, was found to be quite small. In addition, the calculated recovery values are quite high and their standard deviations are small enough. The results obtained with the help of the applied chemometric method are extremely fast, simple and reliable results.

Keywords

Drug, Levadopa, Benserazid, Artificial Neural Network

Parkinson Tedavisinde Kullanılan Levadopa ve Benserazid Etken Maddelerinin Yapay Sinir Ağları Yöntemi ile Tayini

Abstract

Bu çalışmada, tablet ilaç numunesinde bulunan levadopa ve benserazid etken maddelerinin aynı anda miktar tayinlerine hiçbir ayırma işlemi uygulanmaksızın ultraviyole görünür bölge spektroskopisi ile tayinleri gerçekleştirilmiş ve elde edilen veriler yapay sinir ağları (ANN) kemometrik yöntemi ile değerlendirilmiştir. Çalışmanın sonunda kullanılan yöntemi karşılaştırmak amacıyla kısmi en küçük kareler yöntemi (PLS) uygulanmıştır. Yapılan çalışmada etken maddelerin saf haldeki spektrumları elde edilmiş takiben sentetik karışımları hazırlanmıştır. Bu karışımlardan elde edilen veriler ANN ile değerlendirilmiş ve yöntem piyasadan alınan tablet numunesine uygulanmıştır. Çalışmada elde edilen veriler istatistiksel olarak da incelenmiş, ANN yönteminde önemli bir veri grubu olan karekök ortalama hata (RMS) verileri oldukça küçük bulunmuştur. Ayrıca hesaplanan geri kazanım değerleri oldukça yüksek, standart sapmaları ise yeterince küçüktür. Uygulanan kemometrik yöntem yardımıyla elde edilen sonuçlar son derece hızlı, basit ve güvenilir sonuçlardır.

Keywords

İlaç, Levadopa, Benserazid, Yapay sinir ağları

References

• [1] Cheng, F. K. 2017. The use of acupuncture in patients with Parkinson's disease. Geriatric Nursing, 38(4), 302-314. DOI: 10.1016/j.gerinurse.2016.11.010
• [2] Graham, P., 2013. An Introduction to Medicinal Chemistry. Çeviri Editörü Gürsoy, V.A., Bilim Yayınları, Ankara.
• [3] Bhatnagar, P., Vyas, D., Sinha, S.K., Gajbhiye, A. 2017. HPLC Method for Estimation of Drug Release of Entacapone, Levodopa and Cardıdopa Tablets. International Journal of Pharmaceutical and Research. 8(4), 1734-1743. DOI: 10.13040/ IJPSR. 0975-8232.8(4). 1734-43
• [4] https://en.wikipedia.org/wiki/Benserazide
• [5] Grange, S., Holford, N. H., Guentert, T. W., 2001. A pharmacokinetic model to predict the PK interaction of L-dopa and benserazide in rats. Pharmaceutical research, 18,(8),1174-1184. DOI: 10.1023/a: 1010935228654
• [6] Grossi, G., Bargossi, A., Lippi, A., Battistoni, R., 1987. A fully automated catecholamines analyzer based on cartridge extraction and HPLC separation. Chromatographia, 24(1), 842-846. DOI: 10.1016/s0021-9673(01)83578-0
• [7] Wollmer, E., Klein, S., 2017. Development and Validation of a Robust and Efficient HPLC Method for the Simultaneous Quantification of Levodopa, Carbidopa, Benserazid and Entacapone in Complex Matrices. Journal of Pharmacy & Pharmaceutical Sciences, 20, 258-269. DOI: 10,18433/J3K923
• [8] Ahmad, M.Z., Hamid K.A., Effendi T.J.B. 2012. "A validated high-performance liquid chromatographic method for the determination of levodopa in rat plasma & its application in pharmacokinetic studies". IEEE Symposium on Business, Engineering and Industrial Applications, 134-139. DOI: 10.1109/ISBEIA.2012.6422854
• [9] Ertokuş, P. G., Mermertas, Z., Akkaya, A. M., 2018. Application of chemometric methods to quantative analysis of drugs used in parkinson’s disease. SF Journal Of Pharmaceutical and Analytical Chemistry, 1, 2, 1-8. ISSN: 2643-8178
• [10] Patil, V. P., Devdhe, S. J., Kawde, R. V., Kulkarni, V. S., Nagmoti, V. J., Patil, R. D., Kale, S. H., 2012. Novel spectrophotometric determination of levodopa with ninhydrin. International Journal of Pharmacy and Pharmaceutical Sciences, 4, 2, 711-713. ISSN: 0975-1491
• [11] Szyrwiel, L., Pap, J. S., Malinka, W., Szewczuk, Z., Kotynia, A., Brasun, J., 2013. Interactions of anti-Parkinson drug benserazid with Zn (II), Cu (II), Fe (II) ions. Journal of pharmaceutical and biomedical analysis, 76, 36-43. DOI:10.1016/ j.jpba. 2012.11. 032
• [12] Uslu B, Özkan SA, 2002. Determination of binary mixtures of levodopa and benserazid in pharmaceuticals by ratio-spectra derivative spectrophotometry. Analytical Letters, 35(2): 303-314. DOI: 10,1081/AL-120002531
• [13] Zapata-Urzua C, Perez-Ortiz M, Bravo M, Olivieri AC, Alvarez-Lueje A, 2010. Simultaneous voltametric determination of levodopa, carbidopa and benserazid in pharmaceuticals using multivariate calibration. Talanta, 82: 962- 968. DOI: 10.1016/j.talanta.2010.05.071
• [14] Savan Kuyumcu E, Erdoğdu G, 2017. Simultaneous determination of levodopa and benserazid using poly(3-methylthiophene) and a multi-walled carbon nanotube sensor. Journal of Solid-State Electrochemistry, 21(8): 2209-2217. DOI: 10.1007/s1008-0173549-8
• [15] Aktaş A.H, Yaşar S, 2004. Potentiometric titration of some hydroxylated benzoic acids and cinnamic acids by artificial neural network calibration, Acta Chimica Slovenica, 51, 273-282.