BibTex RIS Cite

COMPARISON OF ASTAXANTHIN EXTRACTABILITY YIELDS FOR DISRUPTED HAEMATOCOCCUS CYSTS

Year 2016, Volume: 18 Issue: 52, 21 - 30, 01.01.2016

Abstract

This comprehensive study focusing on the extractability of astaxanthin from Haematococcus cysts was carried out to compare the effectiveness of the cell disruption procedures. Both single step and combined cell disruption procedures were evaluated. A physical, chemical, enzymatic or mechanical disruption process was applied prior to mechanical disruption (bead vortex homogenizer or tissue homogenizer) for the combined cell disruption procedures. In this study the best result was obtained with the consecutive mechanical disruption processes using the combined procedure of tissue homogenizer and bead vortex homogenizer, yielding 68.32 mg Ast/g DW which was 31% higher than that obtained with the single step bead vortex homogenizer treatment. It was concluded that the combined disruption procedures were more effective than the single step disruption procedures with respect to the extractability of astaxanthin from Haematococcus cysts

References

  • Guerin M, Huntley ME, Olaizola M. Haematococcus Astaxanthin: Applications for Human Health and Nutrition, Trends Biotechnology, Vol. 21, 2003, pp.210–216.
  • Richmond A. Handbook of Microalgal Culture: Biotechnology and Applied Phycology, Iowa: Iowa State Press, 2004.
  • Harker M, Tsavalos AJ, Young AJ. Autotrophic Growth and Carotenoid Production of Haematococcus Pluvialis in a 30 Liter Air-Lift Photobioreactor, Journal of Fermentation and Bioengineerig, Vol. 82, 1996, pp.113–118.
  • Santos MF, Mesquita JF. Ultrastructural Study of Haematococcus Lacustris (Girod) Rostafinski (Volvocales). I. Some Aspects of Carotenogenesis, Cytologia, Vol. 49, 1994, pp.215-228.
  • Margalith P. Production of Ketocarotenoids by Microalgae, Applied Microbiology and Biotechnology, Vol. 51, 1999, pp.431-438.
  • Fabregas J, Dominguez A, Maseda A, Otero A. Interactions between Irradiance and Nutrient Availability during astaxanthin Accumulation and Degradation in Haematococcus Pluvialis, Applied Microbiology and Biotechnology, Vol. 61, 2003, pp.545–551.
  • Kobayashi M, Kakizono T, Nishio N, Nagai S. Effects of Light Intensity, Light Quality, and Illumination Cycle on Astaxanthin Formation in a Green Alga Haematococcus Pluvialis, Journal of Fermentation and Bioengineerig, Vol. 74, 1992, pp.61–63.
  • Harker M, Tsavalos AJ, Young AJ. Factors Responsible for Astaxanthin Formation in the Chlorophyte Haematococcus Pluvialis, Bioresource Technology, Vol. 55, 1996, pp.207–214.
  • Lovitt RW, Jones M, Collins SE, Coss GM, Yau CP, Attouch C. Disruption of Baker’s Yeast Using a Disrupter of Simple and Novel Geometry, Process Biochemistry, Vol. 36, 2000, pp.415–421.
  • Balasundaram B, Pandit AB. Selective Release of Invertase by Hydrodynamic Cavitation, Biochemical Engineering Journal, Vol. 8, 2001, pp.251–256.
  • Dickson RC, Dickson LR, Markin JS. Purification and Properties of an Inducible β- Galactosidase Isolated from the Yeast Kluyveromyces Lactis, Journal of Bacteriology, Vol. 137, 1979, pp.51–61.
  • Brady D, Marchant R, McHale L, McHale AP. Isolation and Partial Characterization of β-Galactosidase Activity Produced by a Thermotolerant Strain of Kluyveromyces Marxianus during Growth on Lactose-Containing Media, Enzyme and Microbial Technology, Vol. 17, 1995, pp.696–699.
  • Lind DL, Daniel RM, Cowan DA, Morgan HW. A Thermostable ß-Galactosidase from a Strain of the Anaerobic Thermophile Thermoanaerobacter, Enzyme and Microbial Technology, Vol. 11, 1989, pp.180–186.
  • Stred’anský M, Tomáska M, Sturdík E, Kremnický L. Optimization of β-Galactosidase Extraction from Kluyveromyces Marxianus, Enzyme and Microbial Technology, Vol. 15, 1993, pp.1063–1065.
  • Belo I, Santos JAL, Cabral JMS, Mota M. Optimization Study of Escherichia Coli TB1 Cell Disruption for Cytochrome b5 Recovery in a Small-Scale Bead Mill, Biotechnology Progress, Vol. 12, 1996, pp.201-204.
  • Rippka R, Deruelles JB, Herdman M, Stanier RY. Assignments Strain History and Properties of Pure Cultures of Cyanobacteria, Journal of General Microbiology, Vol. 111, 1979, pp.1-61.
  • Dalay M, Imamoglu E, Demirel Z. Agricultural Fertilizers as Economical Alternative for Cultivation of Haematococcus Pluvialis, J Microbiology and Biotechnology, Vol. 17, 2007, pp.393–397.
  • Kobayashi M, Kurimura Y, Sakamoto Y, Tsuji Y, Selective Extraction of Astaxanthin and chlorophyll from the Green Alga Haematococcus Pluvialis, Biotechnology Technology, Vol. 11, 1997, pp.657-660.
  • Mendes-Pinto M, Raposo MFJ, Bowen J, Young AJ, Morais R. Evaluation of Different Cell Disruption Processes on Encysted Cells of Haematococcus Pluvialis: Effects on Astaxanthin Recovery and Implications for Bio-availability, Joural of Applied Phycology, Vol. 13, 2001, pp.19–24.
  • Choubert G, Heinrich O. Carotenoid Pigments of the Green Alga Haematococcus Pluvialis: Assay on Rainbow Trout, Oncorhynchus Mykiss, Pigmentation in Comparison with Synthetic Astaxanthin and Canthaxanthin, Aquaculture, Vol. 112, 1993, pp.217-226.
  • Hagen C, Braune W, Birckner E, Nuske J. Functional Aspects of Secondary Carotenoids in Haematococcus Lacustris (Girod) Rostafinski (Volvocales), New Phytology, Vol. 125, 1993, pp.625-633.
  • Kobayashi M, Kakizono T, Nagai S. Enhanced Carotenoid Biosynthesis by Oxidative Stress in Acetate-Induced Cyst Cells of a Green Unicellular Alga, Haematococcus pluvialis, Applied Environmental Microbiology, Vol. 59, 1993, pp.867-873.
  • Xylander M, Braune W. Influence of Nickel on the Green Alga Haematococcus Lacustris Rostafinski in Phases of its Life Cycle, Journal of Plant Physiology, Vol. 144, 1994, pp.86-93.
  • Bar E, Rise M, Vishkautsan M, Arad S. Pigment and Structural Changes in Chlorella Zofingiensis upon Light and Nitrogen Stress, Journal of Plant Physiology, Vol. 146, 1995, pp.527-534.
  • Rise M, Cohen E, Vishkautsan M, Cojocarum M, Gottlieb HE, Arad S. Accumulation of Secondary Carotenoids in Chlorella Zofingiensis, Journal of Plant Physiology, Vol. 144, 1994, pp.287-292.
  • Grung M, D'Souza FML, Borowitzka M, Liaaen-Jensen S. Algal Carotenoids 51. Secondary Carotenoids 2. Haematococcus Pluvialis Aplanospores as a Source of (3S, 30S)-Astaxanthin Esters, Journal of Applied Phycology, Vol. 4, 1992, pp.165-171.
  • Yuan JP, Chen F. Purification of Trans-Astaxanthin from a High-Yielding Astaxanthin Ester-Producing Strain of the Microalga Haematococcus Pluvialis, Food Chemistry, Vol. 68, 2000, pp.443-448.
  • Fàbregas J, Domìnguez A, Regueiro M. Optimization of Culture Medium for the Continuous Cultivation of the Microalga Haeamatococcus Pluvialis, Applied Microbiology and Biotechnology, Vol. 53, 2000, pp.530-535.
  • Keshavarz E, Hoare M. Dunnill P. Biochemical Engineering Aspects of Cell Disruption, Chichester: Chichester Horwood press, 1987.
  • Becerra M, Rodríguez-Belmonte E, Cerdán MA, Siso MIG. Extraction of Intracellular Proteins from Kluyveromyces Lactis, Food Technology and Biotechnology, Vol. 39, 2001, pp.135–139.
  • Choe WS, Middelberg APJ. Direct Chemical Extraction of a Recombinant Viral Coat Protein from Escherichia Coli at High Cell Density, Biotechnology and Bioengineering, Vol.75, 2001, pp.451–455.
  • Park PK, Kim EY, Chu KH. Chemical Disruption of Yeast Cells for the Isolation of Carotenoid Pigments, Seperation and Purification Technology, Vol. 53, 2007, pp.148– 152.
  • Yuan JP, Chen F. Hydrolysis Kinetics of Astaxanthin Esters and Stability of Astaxanthin of Haematococcus Pluvialis during Saponification, Journal of Agricultural and Food Chemistry, Vol. 47, 1999, pp.31-35.
  • Gurpilhares DB, Francislene AH, Pessoa AJ, Inˆes CR. Optimization of Glucose-6- Phosphate Dehydrogenase Releasing from Candida Guilliermondii by Disruption with Glass Beads, Enzyme and Microbial Technology, Vol. 39, 2006, pp.591–595.
  • Geciova J, Bury D, Jelen P. Methods for Disruption of Microbial Cells for Potential Use in the Dairy Industry, International Dairy Journal, Vol. 12, 2002, pp.541–553.
  • Doulah M.S. Mechanism of Disintegration of Biological Cells in Ultrasonic Cavitation, Biotechnology and Bioengineering, Vol. 19, 1977, pp.649–660.

PARÇALANMIŞ HAEMATOCOCCUS KİSTLERİ İÇİN ASTAKSANTİN EKSTRAKT VERİMLERİNİN KARŞILAŞTIRILMASI

Year 2016, Volume: 18 Issue: 52, 21 - 30, 01.01.2016

Abstract

Bu çalışmanın ana amacı, minimum hücre içi metabolit kaybı ile maksimum ürün elde edilmesidir. Hücre parçalama teknikleri karşılaştırırlarak Haematococcus kistlerinden astaksantin ekstrakte edilebilirliği araştırılmıştır. Astaksantin eldesi üzerine fiziksel, kimyasal ve mekanik hücre parçalama işlemleri değerlendirilmiştir. Sonuç olarak, 68.32 mg Ast/g DW astaksantin verimi ile kombine prosedür olan doku homojenizatörü takiben bilyalı vorteks homojenizatör uygulamasında en iyi verime ulaşılmıştır. Bu çalışmada kombine hücre parçalama işleminde tek basamaklı hücre parçalama işlemine göre daha başarılı olunmuştur

References

  • Guerin M, Huntley ME, Olaizola M. Haematococcus Astaxanthin: Applications for Human Health and Nutrition, Trends Biotechnology, Vol. 21, 2003, pp.210–216.
  • Richmond A. Handbook of Microalgal Culture: Biotechnology and Applied Phycology, Iowa: Iowa State Press, 2004.
  • Harker M, Tsavalos AJ, Young AJ. Autotrophic Growth and Carotenoid Production of Haematococcus Pluvialis in a 30 Liter Air-Lift Photobioreactor, Journal of Fermentation and Bioengineerig, Vol. 82, 1996, pp.113–118.
  • Santos MF, Mesquita JF. Ultrastructural Study of Haematococcus Lacustris (Girod) Rostafinski (Volvocales). I. Some Aspects of Carotenogenesis, Cytologia, Vol. 49, 1994, pp.215-228.
  • Margalith P. Production of Ketocarotenoids by Microalgae, Applied Microbiology and Biotechnology, Vol. 51, 1999, pp.431-438.
  • Fabregas J, Dominguez A, Maseda A, Otero A. Interactions between Irradiance and Nutrient Availability during astaxanthin Accumulation and Degradation in Haematococcus Pluvialis, Applied Microbiology and Biotechnology, Vol. 61, 2003, pp.545–551.
  • Kobayashi M, Kakizono T, Nishio N, Nagai S. Effects of Light Intensity, Light Quality, and Illumination Cycle on Astaxanthin Formation in a Green Alga Haematococcus Pluvialis, Journal of Fermentation and Bioengineerig, Vol. 74, 1992, pp.61–63.
  • Harker M, Tsavalos AJ, Young AJ. Factors Responsible for Astaxanthin Formation in the Chlorophyte Haematococcus Pluvialis, Bioresource Technology, Vol. 55, 1996, pp.207–214.
  • Lovitt RW, Jones M, Collins SE, Coss GM, Yau CP, Attouch C. Disruption of Baker’s Yeast Using a Disrupter of Simple and Novel Geometry, Process Biochemistry, Vol. 36, 2000, pp.415–421.
  • Balasundaram B, Pandit AB. Selective Release of Invertase by Hydrodynamic Cavitation, Biochemical Engineering Journal, Vol. 8, 2001, pp.251–256.
  • Dickson RC, Dickson LR, Markin JS. Purification and Properties of an Inducible β- Galactosidase Isolated from the Yeast Kluyveromyces Lactis, Journal of Bacteriology, Vol. 137, 1979, pp.51–61.
  • Brady D, Marchant R, McHale L, McHale AP. Isolation and Partial Characterization of β-Galactosidase Activity Produced by a Thermotolerant Strain of Kluyveromyces Marxianus during Growth on Lactose-Containing Media, Enzyme and Microbial Technology, Vol. 17, 1995, pp.696–699.
  • Lind DL, Daniel RM, Cowan DA, Morgan HW. A Thermostable ß-Galactosidase from a Strain of the Anaerobic Thermophile Thermoanaerobacter, Enzyme and Microbial Technology, Vol. 11, 1989, pp.180–186.
  • Stred’anský M, Tomáska M, Sturdík E, Kremnický L. Optimization of β-Galactosidase Extraction from Kluyveromyces Marxianus, Enzyme and Microbial Technology, Vol. 15, 1993, pp.1063–1065.
  • Belo I, Santos JAL, Cabral JMS, Mota M. Optimization Study of Escherichia Coli TB1 Cell Disruption for Cytochrome b5 Recovery in a Small-Scale Bead Mill, Biotechnology Progress, Vol. 12, 1996, pp.201-204.
  • Rippka R, Deruelles JB, Herdman M, Stanier RY. Assignments Strain History and Properties of Pure Cultures of Cyanobacteria, Journal of General Microbiology, Vol. 111, 1979, pp.1-61.
  • Dalay M, Imamoglu E, Demirel Z. Agricultural Fertilizers as Economical Alternative for Cultivation of Haematococcus Pluvialis, J Microbiology and Biotechnology, Vol. 17, 2007, pp.393–397.
  • Kobayashi M, Kurimura Y, Sakamoto Y, Tsuji Y, Selective Extraction of Astaxanthin and chlorophyll from the Green Alga Haematococcus Pluvialis, Biotechnology Technology, Vol. 11, 1997, pp.657-660.
  • Mendes-Pinto M, Raposo MFJ, Bowen J, Young AJ, Morais R. Evaluation of Different Cell Disruption Processes on Encysted Cells of Haematococcus Pluvialis: Effects on Astaxanthin Recovery and Implications for Bio-availability, Joural of Applied Phycology, Vol. 13, 2001, pp.19–24.
  • Choubert G, Heinrich O. Carotenoid Pigments of the Green Alga Haematococcus Pluvialis: Assay on Rainbow Trout, Oncorhynchus Mykiss, Pigmentation in Comparison with Synthetic Astaxanthin and Canthaxanthin, Aquaculture, Vol. 112, 1993, pp.217-226.
  • Hagen C, Braune W, Birckner E, Nuske J. Functional Aspects of Secondary Carotenoids in Haematococcus Lacustris (Girod) Rostafinski (Volvocales), New Phytology, Vol. 125, 1993, pp.625-633.
  • Kobayashi M, Kakizono T, Nagai S. Enhanced Carotenoid Biosynthesis by Oxidative Stress in Acetate-Induced Cyst Cells of a Green Unicellular Alga, Haematococcus pluvialis, Applied Environmental Microbiology, Vol. 59, 1993, pp.867-873.
  • Xylander M, Braune W. Influence of Nickel on the Green Alga Haematococcus Lacustris Rostafinski in Phases of its Life Cycle, Journal of Plant Physiology, Vol. 144, 1994, pp.86-93.
  • Bar E, Rise M, Vishkautsan M, Arad S. Pigment and Structural Changes in Chlorella Zofingiensis upon Light and Nitrogen Stress, Journal of Plant Physiology, Vol. 146, 1995, pp.527-534.
  • Rise M, Cohen E, Vishkautsan M, Cojocarum M, Gottlieb HE, Arad S. Accumulation of Secondary Carotenoids in Chlorella Zofingiensis, Journal of Plant Physiology, Vol. 144, 1994, pp.287-292.
  • Grung M, D'Souza FML, Borowitzka M, Liaaen-Jensen S. Algal Carotenoids 51. Secondary Carotenoids 2. Haematococcus Pluvialis Aplanospores as a Source of (3S, 30S)-Astaxanthin Esters, Journal of Applied Phycology, Vol. 4, 1992, pp.165-171.
  • Yuan JP, Chen F. Purification of Trans-Astaxanthin from a High-Yielding Astaxanthin Ester-Producing Strain of the Microalga Haematococcus Pluvialis, Food Chemistry, Vol. 68, 2000, pp.443-448.
  • Fàbregas J, Domìnguez A, Regueiro M. Optimization of Culture Medium for the Continuous Cultivation of the Microalga Haeamatococcus Pluvialis, Applied Microbiology and Biotechnology, Vol. 53, 2000, pp.530-535.
  • Keshavarz E, Hoare M. Dunnill P. Biochemical Engineering Aspects of Cell Disruption, Chichester: Chichester Horwood press, 1987.
  • Becerra M, Rodríguez-Belmonte E, Cerdán MA, Siso MIG. Extraction of Intracellular Proteins from Kluyveromyces Lactis, Food Technology and Biotechnology, Vol. 39, 2001, pp.135–139.
  • Choe WS, Middelberg APJ. Direct Chemical Extraction of a Recombinant Viral Coat Protein from Escherichia Coli at High Cell Density, Biotechnology and Bioengineering, Vol.75, 2001, pp.451–455.
  • Park PK, Kim EY, Chu KH. Chemical Disruption of Yeast Cells for the Isolation of Carotenoid Pigments, Seperation and Purification Technology, Vol. 53, 2007, pp.148– 152.
  • Yuan JP, Chen F. Hydrolysis Kinetics of Astaxanthin Esters and Stability of Astaxanthin of Haematococcus Pluvialis during Saponification, Journal of Agricultural and Food Chemistry, Vol. 47, 1999, pp.31-35.
  • Gurpilhares DB, Francislene AH, Pessoa AJ, Inˆes CR. Optimization of Glucose-6- Phosphate Dehydrogenase Releasing from Candida Guilliermondii by Disruption with Glass Beads, Enzyme and Microbial Technology, Vol. 39, 2006, pp.591–595.
  • Geciova J, Bury D, Jelen P. Methods for Disruption of Microbial Cells for Potential Use in the Dairy Industry, International Dairy Journal, Vol. 12, 2002, pp.541–553.
  • Doulah M.S. Mechanism of Disintegration of Biological Cells in Ultrasonic Cavitation, Biotechnology and Bioengineering, Vol. 19, 1977, pp.649–660.
There are 36 citations in total.

Details

Other ID JA35NT28BU
Journal Section Research Article
Authors

Bahar Aslanbay This is me

Esra İmamoğlu This is me

Publication Date January 1, 2016
Published in Issue Year 2016 Volume: 18 Issue: 52

Cite

APA Aslanbay, B., & İmamoğlu, E. (2016). PARÇALANMIŞ HAEMATOCOCCUS KİSTLERİ İÇİN ASTAKSANTİN EKSTRAKT VERİMLERİNİN KARŞILAŞTIRILMASI. Dokuz Eylül Üniversitesi Mühendislik Fakültesi Fen Ve Mühendislik Dergisi, 18(52), 21-30.
AMA Aslanbay B, İmamoğlu E. PARÇALANMIŞ HAEMATOCOCCUS KİSTLERİ İÇİN ASTAKSANTİN EKSTRAKT VERİMLERİNİN KARŞILAŞTIRILMASI. DEUFMD. January 2016;18(52):21-30.
Chicago Aslanbay, Bahar, and Esra İmamoğlu. “PARÇALANMIŞ HAEMATOCOCCUS KİSTLERİ İÇİN ASTAKSANTİN EKSTRAKT VERİMLERİNİN KARŞILAŞTIRILMASI”. Dokuz Eylül Üniversitesi Mühendislik Fakültesi Fen Ve Mühendislik Dergisi 18, no. 52 (January 2016): 21-30.
EndNote Aslanbay B, İmamoğlu E (January 1, 2016) PARÇALANMIŞ HAEMATOCOCCUS KİSTLERİ İÇİN ASTAKSANTİN EKSTRAKT VERİMLERİNİN KARŞILAŞTIRILMASI. Dokuz Eylül Üniversitesi Mühendislik Fakültesi Fen ve Mühendislik Dergisi 18 52 21–30.
IEEE B. Aslanbay and E. İmamoğlu, “PARÇALANMIŞ HAEMATOCOCCUS KİSTLERİ İÇİN ASTAKSANTİN EKSTRAKT VERİMLERİNİN KARŞILAŞTIRILMASI”, DEUFMD, vol. 18, no. 52, pp. 21–30, 2016.
ISNAD Aslanbay, Bahar - İmamoğlu, Esra. “PARÇALANMIŞ HAEMATOCOCCUS KİSTLERİ İÇİN ASTAKSANTİN EKSTRAKT VERİMLERİNİN KARŞILAŞTIRILMASI”. Dokuz Eylül Üniversitesi Mühendislik Fakültesi Fen ve Mühendislik Dergisi 18/52 (January 2016), 21-30.
JAMA Aslanbay B, İmamoğlu E. PARÇALANMIŞ HAEMATOCOCCUS KİSTLERİ İÇİN ASTAKSANTİN EKSTRAKT VERİMLERİNİN KARŞILAŞTIRILMASI. DEUFMD. 2016;18:21–30.
MLA Aslanbay, Bahar and Esra İmamoğlu. “PARÇALANMIŞ HAEMATOCOCCUS KİSTLERİ İÇİN ASTAKSANTİN EKSTRAKT VERİMLERİNİN KARŞILAŞTIRILMASI”. Dokuz Eylül Üniversitesi Mühendislik Fakültesi Fen Ve Mühendislik Dergisi, vol. 18, no. 52, 2016, pp. 21-30.
Vancouver Aslanbay B, İmamoğlu E. PARÇALANMIŞ HAEMATOCOCCUS KİSTLERİ İÇİN ASTAKSANTİN EKSTRAKT VERİMLERİNİN KARŞILAŞTIRILMASI. DEUFMD. 2016;18(52):21-30.

Dokuz Eylül Üniversitesi, Mühendislik Fakültesi Dekanlığı Tınaztepe Yerleşkesi, Adatepe Mah. Doğuş Cad. No: 207-I / 35390 Buca-İZMİR.