The Effect of Natural Antimicrobial Agents on the Characteristics of Surgical Sutures

Year 2020, Volume: 22 Issue: 64, 11 - 20, 24.01.2020

Elif Alyamaç Seydibeyoğlu , Ayşe Işık

https://doi.org/10.21205/deufmd.2020226402

Cited By: 4

Abstract

Surgical site infections (SSI)
occur after the surgery in body parts where the operation took place. In surgeries,
wounds are closed by thread-like materials known as sutures. Some types of
sutures may promote bacteria proliferation which is one of the leading causes
of the SSI. Sutures undergo coating procedure to prevent infection occurrence. In
this study, different types of surgical sutures were coated with natural
antimicrobial agents to evaluate their effect on morphological and mechanical
properties of the surgical sutures. In this context, due to its antimicrobial
ability, chitosan was selected and dissolved in acetic acid solution with other
natural antimicrobial agents (aloe vera and olive leaf extract) through
ultrasound technology. Multifilament silk, multifilament polyester, and
monofilament polyamide sutures were then dipped into those solutions prepared
at different concentrations in order to study the synergistic effect of
antimicrobial agents.  Fourier transform
infrared spectroscopy with attenuated total reflectance (FTIR-ATR) was performed
to identify the functional groups on the surface of the coated sutures. Suture
surfaces were also analyzed by scanning electron microscope (SEM) to observe the
coating on the surface of sutures. Strong adhesion was determined between the
suture surface and the coating material after long duration of dipping and
drying procedure. It was also found that the coating process increased the
mechanical properties of the sutures.

Keywords

Biopolymer, Chitosan, Aloe Vera, Olive Leaf Extract, Surgical Site Infections, Mechanical Properties

Doğal Antimikrobiyal Maddelerin Cerrahi Sütürlerin Özelliklerine Etkisi

Abstract

Cerrahi girişim uygulanan vücut bölgelerinde, ameliyat sonrası cerrahi alan enfeksiyonları (CAE) ortaya çıkar. Cerrahide yaralar sütür olarak bilinen iplik benzeri materyallerle kapatılır. Bazı sütür tipleri, CAE'ye sebep olan bakterilerin çoğalmasına neden olmaktadır. Sütür kaynaklı enfeksiyonun önüne geçmek için antimikrobiyallerle kaplama yapılır. Bu çalışmada, CAE’nin üstesinden gelmek amacıyla farklı tipteki cerrahi sütürler doğal antimikrobiyal ajanlarla kaplanmıştır ve bu ajanların cerrahi sütürlerin morfolojik ve mekanik özelliklerine olan etkisi değerlendirilmiştir. Bu bağlamda, doğal bir polimer olan kitosan antimikrobiyal özelliğe sahip olması sebebiyle seçilmiştir ve kitosan yine doğal antimikrobiyal ajan olan aloe vera ve zeytin yaprağı ekstresi ile beraber asetik asit çözeltisi içerisinde ultrason teknolojisi kullanılarak karıştırılıp kaplama solüsyonu hazırlanmıştır. Doğal ajanların sinerjistik etkisini çalışmak için farklı konsantrasyonlarda hazırlanan kaplama solüsyonlarına multifilament ipek, multifilament poliester ve monofilament poliamid sütürler daldırılarak kaplama yapılmıştır. Fourier dönüşümlü kızılötesi spektroskopisi kullanılarak kaplanmış sütürlerin üzerindeki fonksiyonel gruplar belirlenmiştir. Sütürlerin yüzeyleri taramalı elektron mikroskobuyla incelenerek uzun daldırma ve kurutma işlemleri sonrası kaplama malzemesinin yüzeye güçlü bir şekilde tutunduğu gözlemlenmiştir. Ayrıca yapılan mekanik testler sonucunda kaplama işleminin sütür mekanik özelliklerini arttırdığı tespit edilmiştir.

 

Keywords

Biyopolimer, Kitosan, Aloe Vera, Zeytin Yaprağı Ekstresi, Cerrahi Alan Enfeksiyonları, Mekanik Özellikler

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