Rodamin 123 Etiketli Bor Nitrür Nanoparçacıklarının Karakterizasyonu ve in vitro Biyogörüntüleme Potansiyelinin Araştırılması

Year 2021, Volume: 23 Issue: 68, 595 - 605, 24.05.2021

Erdal Eroğlu

https://doi.org/10.21205/deufmd.2021236821

Abstract

Bu çalışmanın amacı bor nitrür (BN) nanoparçacıklarının floresan bir boya olan rodamin 123 (Rd) ile etiketlenmesi ve biyogörüntüleme amaçlı kullanımının in vitro ortamda araştırılmasıdır. Öncelikli olarak, rodamin 123 etiketi bor nitrür nanoparçacıklarına glutaraldehid çapraz bağlayıcısı aracılığıyla bağlanarak BN-Rd nanoparçacıkları üretilmiş ve SEM, UV-görünür bölge spektroskopisi, MTT hücre proliferasyon testi, ışık mikroskobu ve floresan mikroskobu analizleri ile karakterize edilmiştir. Sitotoksik olmayan konsantrasyonlardaki BN-Rd nanoparçacıkları insan prostat kanseri hücreleri (PC-3) ile insan embriyonik böbrek hücreleri (HEK-293) üzerine uygulanmış ve hücreler floresan mikroskobu altında incelenerek BN-Rd’nin biyogörüntüleme potansiyeli in vitro ortamda araştırılmıştır. Sonuç olarak, SEM analizlerinde ~40 nm boyuta sahip olduğu belirlenen BN ve BN-Rd 30 µg/ml ve üzerindeki konsantrasyonlarda sitotoksik etki göstermiştir. Işık mikroskobu altında incelenen nanoparçacık uygulanmış hücre morfolojileri MTT sonuçlarını destekler nitelikte bulunmuştur. Her iki hücre hattı üzerine de uygulanan BN-Rd’nin 10 µg/ml konsantrasyonda hücreler ile etkileşime geçtiği ve hücrelerin in vitro ortamda floresan mikroskobu altında ışıma yaptığı gözlenmiştir. Bu çalışmadan elde edilen bulgular ışığında rodamin 123 floresan boyası ile etiketli bor nitrür nanoparçacıklarının biyogörüntüleme uygulamalarında kullanım potansiyeli ortaya konmuştur.

Keywords

bor nitrür nanoparçacıkları, rodamin 123, biyogörüntüleme

Thanks

Teşekkür Bu çalışmanın ön deneme sürecine katkı sağlayan İzmir Özel Yeşeren Fen Lisesi öğretmenlerinden Adil CAN’a ve öğrencisi İdil Bilge TUNÇ’a teşekkür ederim.

Characterization of Rhodamine 123-Labeled Boron Nitride Nanoparticles and Investigation of in vitro Bioimaging Potential

Abstract

The aim of this study is to label boron nitride (BN) nanoparticles with a fluorescent dye, rhodamine 123 (Rd), and to investigate its use for bioimaging in vitro condition. First, BN-Rd nanoparticles were produced by binding rhodamine 123 tag to boron nitride nanoparticles via glutaraldehyde crosslinker and characterized by SEM, UV-visible spectroscopy, MTT cell proliferation test, light microscope and fluorescence microscope analyses. BN-Rd nanoparticles at non-cytotoxic concentrations were applied on human prostate cancer cells (PC-3) and human embriyonic kidney cells (HEK-293) and the bioimaging potential of BN-Rd was investigated in vitro by examining the cells under fluorescence microscopy. As a result, BN and BN-Rd, which were determined to have a size of ~40 nm in SEM analysis, showed a cytotoxic effect at concentrations of 30 µg/ml and above. The nanoparticle-treated cell morphologies examined under a light microscope was found to support MTT results. It was observed that BN-Rd, at a concentration of 10 µg/ml applied on both cell lines interacted with the cells and the cells fluoresced under a fluorescence microscope in vitro. In the light of the findings obtained from this study, the potential use of boron nitride nanoparticles labeled with rhodamine 123 fluorescent dye in bioimaging applications has been revealed.

Keywords

Boron Nitride Nanoparticles, Rhodamine 123, Bioimaging

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