Determination of Antibacterial and Antifungal Properties of Epilobium hirsutum L. (Fam: Onagraceae)

Year 2024, , 51 - 61, 08.08.2024

Mesut Sırrı , Bülent Hallaç , Mehmet Fidan

https://doi.org/10.57244/dfbd.1487289

Abstract

In recent years, considering the spread of antibiotic-resistant pathogens and the growing concern, there has been a focus on researching natural plant-derived compounds as alternatives to synthetics. This study aims to determine the antibacterial and antifungal properties of the aerial parts of Epilobium hirsutum L., naturally grown in the Gever plain of Hakkâri/Yüksekova District, using ethanol, methanol, and water extracts. The antimicrobial activities of E. hirsutum extracts (ethanol, methanol, and water) were tested in vitro against six microorganisms (Bacillus subtilis ATCC 6633, Escherichia coli ATCC 25922, Enterococcus faecalis ATCC 29242, and Staphylococcus aureus ATCC 25923 bacteria, as well as Candida albicans ATCC 10231 and Saccharomyces cerevisiae ATCC 9763 fungi). In the study, it was determined that the methanol and water extracts of the plant exhibited antimicrobial activities against E. coli and E. faecalis, creating inhibition zones of 13.10 mm and 12.26 mm, respectively. The ethanol extract showed antifungal activities against C. albicans and S. cerevisiae, creating inhibition zones in the range of 8-11 mm. Overall, the extracts of E. hirsutum were found to have a moderate effect compared to Amikacin, Streptomycin, and Nystatin standards. In conclusion, it can be stated that the extracts of E. hirsutum have a potential impact for use in pharmaceuticals, food, and other industrial applications as antibacterial and antifungal agents.

Keywords

Hairy willowherb, weed, extract, antimicrobial, Yüksekova/Hakkâri

Epilobium hirsutum L. Türünün Antibakteriyel ve Antifungal Özelliklerinin Belirlenmesi

Abstract

Son yıllarda antibiyotiklere karşı dirençli patojenlerin yayılması ve artan endişe göz önüne alındığında, sentetiklere alternatif olarak doğal bitki kökenli bileşikleri araştırmak ilgi odağı olmuştur. Bu çalışmada, Hakkari/Yüksekova İlçesi Gever ovasında doğal olarak yetişen Epilobium hirsutum L. türünün toprak üstü kısımlarının etanol, metanol ve su özütlerinin antibakteriyel ve antifungal özelliklerinin belirlenmesi amaçlanmıştır. E. hirsutum'un ekstraktlarının (etanol, metanol ve su) antimikrobiyal aktiviteleri in vitro olarak altı mikroorganizmaya (Bacillus subtilis ATCC 6633, Escherichia coli ATCC 25922, Enterococcus faecalis ATCC 29242 ve Staphylococcus aureus ATCC 25923 bakterileri ile Candida albicans ATCC 10231 ve Saccharomyces cerevisiae ATCC 9763) karşı test edilmiştir. Çalışmada, bitkiye ait metanol ve su özütlerinin E. coli ve E. faecalis’e karşı antimikrobiyal aktiviteleri sırasıyla 13.10 mm ve 12.26 mm inhibisyon zonu oluşturduğu belirlenmiştir. Etanol özütünün C. albicans ve S. cerevisiae karşı antifungal aktiviteleri 8-11 mm aralığında inhibisyon zonu oluşturduğu tespit edilmiştir. Genel olarak E. hirsutum'a ait özütlerin Amikacin, Streptomycin ve Nistatin standartlarına göre orta düzeyde etki gösterdiği saptanmıştır. Sonuç olarak, E. hirsutum'un ekstraktlarının antibakteriyel ve antifungal olarak ilaç, gıda ve diğer endüstriyel uygulamalar için kullanılabilir düzeyde bir etkiye sahip olduğu ifade edilebilir.

Keywords

Epilobium hirsutum, ekstrakt, antimikrobiyal, Yüksekova/Hakkari

References

• Ak, G., Zengin, G., Mahomoodally, M. F., Llorent-Martínez, E., Orlando, G., Chiavaroli, A., ... Ferrante, C. (2021). Shedding light into the connection between chemical components and biological effects of extracts from Epilobium hirsutum: Is it a potent source of bioactive agents from natural treasure?. Antioxidants, 10(9), 1389.
• Bakis, Y., Babac, M. T., Uslu, E. (2011). "Updates and improvements of Turkish Plants Data Service (TÜBİVES)" In Health Informatics and Bioinformatics (HIBIT), 2011 6th International Symposium on IEEE. May, 02-05, 2011 İzmir, Türkiye.
• Barakat, H. H., Hussein, S. A., Marzouk, M. S., Merfort, I., Linscheid, M., Nawwar, M. A. (1997). Polyphenolic metabolites of Epilobium hirsutum. Phytochemistry, 46(5), 935-941.
• Bartfay, W. J., Bartfay, E., Johnson, J. G. (2012). Gram-negative and gram-positive antibacterial properties of the whole plant extract of willow herb (Epilobium angustifolium). Biological Research for Nursing, 14(1), 85-89.
• Baum, D. A., Sytsma, K. J., Hoch, P. C. (1994). A phylogenetic analysis of Epilobium (Onagraceae) based on nuclear ribosomal DNA sequences. Systematic Botany, 19(3), 363-388.
• Bodeker, G., Kronenberg, F. (2002). A public health agenda for traditional, complementary and alternative medicine. American Journal of Public Health, 92, 1582-1591.
• Cando, D., Morcuende, D., Utrera, M., Estévez, M. (2014). Phenolic-rich extracts from Willowherb (Epilobium hirsutum L.) inhibit lipid oxidation but accelerate protein carbonylation and discoloration of beef patties. European Food Research and Technology, 238, 741-751.
• Cemeroğlu, B. S. (2013). Gıda Analizleri, Ankara, 3. Baskı, Bizim Grup Basımevi.
• Dreger, M., Adamczak, A., Foksowicz-Flaczyk, J. (2023). Antibacterial and antimycotic activity of Epilobium angustifolium l. extracts: a review. Pharmaceuticals, 16(10), 1419.
• Dürüst, N., Dürüst, Y., İkinci, N., Banko, S., Hoşgün, E. Z., Bozan, B. (2023). HPLC determination of polyphenols of the flowers of Digitalis lamarckii, Xeranthemum annuum, Epilobium hirsutum and Silene compacta from Bolu (Turkey). Journal of Medicinal Plants, 17(5), 164-179.
• Ege, T. (2019). The pharmacological and therapeutic potentials of Epilobium hirsutum L., International Journal of Pharmaceutical Sciences Review and Research, 57, 20-23.
• Eghmazi, E., Akhgar, M. R., Kariminik, A. (2015). Oil from Epilobium hirsutum. Journal of Biodiversity and Environmental Sciences, 7, 338-344.
• Fidan, H., Fidan, M. (2021). Şırnak Yöresinde Alternatif Tedavi Amaçlı Kullanılan Origanum vulgare L. subsp. gracile (K. Koch) Ietsw. Bitkisinin Element Analizi. Şırnak Üniversitesi Fen Bilimleri Dergisi, 2(1), 23-28.
• Fidan, M., Karaismailoğlu, M. C. (2020). Kenevirin Tarihçesi ve Sistematiği. Palme, Ankara, 1-13.
• Fidan, M., Pınar, S. M., Erez, M. E., İnal, B. (2022). Salvia kurdica Boiss. & Hohen. ex Benth. ve Salvia pachystachys Trautv. türlerinin antioksidan özellikleri ile antibakteriyel etkilerinin belirlenmesi. Doğu Fen Bilimleri Dergisi, 5(2), 19-29.
• Fidan, M., Süzerer, V., Onay, A. (2023). Cannabis sativa L.: origin, distributıon, taxonomy and biology. Türk Bilimsel Derlemeler Dergisi, 16(1), 10-28.
• Ganhão, R., Morcuende, D., Estévez, M. (2010). Protein oxidation in emulsified cooked burger patties with added fruit extracts: influence on colour and texture deterioration during chill storage. Meat Science, 85, 402–409
• Granica S, Piwowarski J.P, Czerwińska M.E., Kiss A.K. (2014). Phytochemistry, pharmacology and traditional uses of different Epilobium species (Onagraceae): a review. Journal of Ethnopharmacology, 156, 316–346. http://dx.doi.org/10.1016/j.jep.2014.08.036
• Güner, A., Aslan, S., Ekim, T., Vural, M., Babaç, M.T. (edlr.), (2012). Türkiye Bitkileri Listesi (Damarlı Bitkiler). Nezahat Gökyiğit Botanik Bahçesi ve Flora Araştırmaları Derneği Yayını. İstanbul.
• Hallaç, B., Kılınççeker, O. (2023). Deve Dikeni (Silybum marianum) Tohumu Ununun Tavuk Köfte Üretiminde Kullanım Olanakları. Süleyman Demirel Üniversitesi Fen Bilimleri Enstitüsü Dergisi, 27(2), 207-212.
• Ikram, M., Ali, N., Jan, G., Jan, F., Romman, M., Ishaq, M., … Khan, N. (2021). Antimicrobial and antioxidant activities of methanolic extract and fractions of Epilobium roseum (Schreb.) against bacterial strains. American Journal of Plant Sciences, 12, 275-284. doi: 10.4236/ajps.2021.123017.
• Karakaya, S., Süntar, I., Yakinci, O. F., Sytar, O., Ceribasi, S., Dursunoglu, B., ... Guvenalp, Z. (2020). In vivo bioactivity assessment on Epilobium species: A particular focus on Epilobium angustifolium and its components on enzymes connected with the healing process. Journal of Ethnopharmacology, 262, 113207.
• Kılıç, G., Korkmaz, B., Erik, İ., Fandaklı, S., Yaylı, S. S., Faiz, Ö., ... Yaylı, N. (2020). Antimicrobial, antioxidant, tyrosinase activities and volatile compounds of the essential oil and solvent extract of Epilobium hirsutum L. growing in Turkey. Turkish Journal of Analytical Chemistry, 2(2), 87-94.
• Kiss, A., Kowalski, J., Melzig, M. F. (2004). Compounds from Epilobium angustifolium inhibit the specific metallopeptidases ACE, NEP and APN. Planta medica, 70(10), 919-923.
• Kiss, A.K., Bazylko, A., Filipek, A., Granica, S., Jaszewska, E., Kiarszys, U., … Piwowarski J. (2011). Oenothein Bʼs contribution to the anti-inflammatory and antioxidant activity of Epilobium sp. Phytomedicine, 18, 557-560.
• Mohammadi Bazargani, M., Falahati-Anbaran, M., Rohloff, J. (2021). Comparative analyses of phytochemical variation within and between congeneric species of willow herb, Epilobium hirsutum and E. parviflorum: Contribution of environmental factors. Frontiers in plant science, 11, 595190.
• Moreira, M. R., Ponce, A. G., Del Valle, C. E., Roura, S. I. (2005). Inhibitory parameters of essential oils to reduce a foodborne pathogen. LWT-Food Science and Technology, 38(5), 565-570.
• Nicu, A. I., Pîrvu, L., Vamanu, A. (2017). Antibacterial activity of ethanolic extracts from Agrimonia eupatoria L. and Epilobium hirsutum L. herba. Scientific Bulletin Series F. Biotechnologies, 21, 127-132.
• Nowak, A., Cybulska, K., Makuch, E., Kucharski, Ł., Różewicka-Czabańska, M., Prowans, P., ... Klimowicz, A. (2021). In vitro human skin penetration, antioxidant and antimicrobial activity of ethanol-water extract of fireweed (Epilobium angustifolium L.). Molecules, 26(2), 329. doi: 10.3390/molecules26020329.
• Önen, H. (2003). Bazı bitkisel uçucu yağların biyoherbisidal etkileri. Turkish Journal of Weed Science, 6, 39-47. Shttps://www.researchgate.net/profile/Huseyin-Onen/publications
• Öztürkcan, A., Acar, S. (2017). A general assessment of widely used antimicrobial food additives. İstanbul Gelişim Üniversitesi Sağlık Bilimleri Dergisi, 1, 1-17.
• Pirvu, L., Nicorescu, V., Hlevca, C., Udeanu, D. I., Nicorescu, I. (2015). Antimicrobial and synergistic activity of some whole and selective Epilobium hirsutum L. (great willowherb) extracts tested on standard and wild Staphylococcus aureus strains. Farmacia, 63(5), 690-695.
• Rodríguez-Carpena, J.G., Morcuende, D., Estévez, M. (2011) Avocado y-products as inhibitors of color deterioration and lipid and protein oxidation in raw porcine patties subjected to chilled storage. Meat Science, 89, 166–173
• Ruszová, E., Cheel, J., Pávek, S., Moravcová, M., Hermannová, M., Matějková, I., ... Kubala, L. (2013). Epilobium angustifolium extract demonstrates multiple effects on dermal fibroblasts in vitro and skin photo-protection in vivo. General Physiology and Biophysics, 32(3), 347-359.
• Schepetkin, I. A., Ramstead, A. G., Kirpotina, L. N., Voyich, J. M., Jutila, M. A., Quinn, M. T. (2016). Therapeutic potential of polyphenols from Epilobium angustifolium (Fireweed). Phytotherapy research, 30(8), 1287-1297.
• Sevgili, S. Ş. (2019). Doğal katkı maddelerinin bitkisel yağların stabilitelerine etkileri. Çukurova Tarım ve Gıda Bilimleri Dergisi, 34(1), 61-68.
• Sırrı, M., Özaslan, C., Fidan, M. (2021). Eruh (Siirt) ilçesinde gıda ve halk tababetinde kullanılan bazı doğal ve yabancı otlar. MAS Journal of Applied Sciences, 6(Özel Sayı), 1118-1129.
• Sırrı, M., Sırrı, G. (2020). Hakkâri ilinde gıda olarak tüketilen yabani bitki ve yabancı ot türlerinin güncel durumu. Avrupa Bilim ve Teknoloji Dergisi, 19, 393-409.
• Stolarczyk, M., Naruszewicz, M., Kiss, A. K. (2013). Extracts from Epilobium sp. herbs induce apoptosis in human hormone-dependent prostate cancer cells by activating the mitochondrial pathway. Journal of Pharmacy and Pharmacology, 65(7), 1044-1054.
• Temiz, A. (2010). Antimikrobiyal Maddelere Duyarlılık. Genel Mikrobiyoloji Uygulama Teknikleri. Ankara, Hatiboğlu Yayınevi.
• Tóth, B. H., Blazics, B., Kéry, Á. (2009). Polyphenol composition and antioxidant capacity of Epilobium species. Journal of Pharmaceutical and Biomedical Analysis, 49(1), 26-31.
• Vitali, F., Fonte, G., Saija, A., Tita, B. (2006). Inhibition of intestinal motility and secretion by extracts of Epilobium spp. in mice. Journal of ethnopharmacology, 107(3), 342-348.
• Vitalone, A., Allkanjari, O. (2018). Epilobium spp.: Pharmacology and Phytochemistry. Phytotherapy Research, 32(7), 1229-1240.
• Vlase, A. M., Toiu, A., Tomuță, I., Vlase, L., Muntean, D., Casian, T., ... Crișan, G. (2022). Epilobium species: from optimization of the extraction process to evaluation of biological properties. Antioxidants, 12(1), 91. https://doi.org/10.3390/antiox12010091
• Wojdyło, A., Oszmiański, J., Czemerys, R. (2007). Antioxidant activity and phenolic compounds in 32 selected herbs. Food chemistry, 105(3), 940-949.
• Zeybek, N., Zeybek, U. (1994). Pharmaceutical Botany, Ege University Faculty of Pharmacy Publication, Turkey.