Ductal carcinoma in situ lesions of the breast: correlation between histopathologic features and p53, HER2/neu, bcl-2 and PCNA expression

Year 2018, , 265 - 273, 05.09.2018

Murat Tad , Sezer Kulaçoğlu

https://doi.org/10.5798/dicletip.457239

Cited By: 1

Abstract

Objective: The histopathological subtype, nuclear grade and comedonecrosis are important features for ductal
carcinoma in situ (DCIS) of the breast. The aim of this study was to determine correlation between histopathologic
properties of DCIS and p53, HER2/neu, bcl-2 and PCNA overexpression.
Method: We evaluated 40 cases of DCIS for p53, HER2/neu, bcl-2 and PCNA overexpression by using
immunohistochemical methods. We divided lesions into two groups. Low/intermediate grade DCIS cases were
included in group 1 (low grade DCIS) and high grade DCIS were included in group 2 (high grade DCIS). Histologic
paterns were also evaluated. Comedo type and non-comedo type DCIS groups were formed.
Results: A total of 40 DCIS including 12 high grade, 28 low grade, 8 comedo type and 32 non-comedo type were
revealed. P53 and HER2/neu overexpressions were seen 37,5%, 62,5% in comedo type DCIS and 6,5%, 3,1% in noncomedo type DCIS, respectively. P53 and HER2/neu overexpressions were not seen in low grade DCIS and were seen
41,7% and 50% in high grade DCIS, respectively. Strong cytoplasmic staining with bcl-2 was not seen in high grade
DCIS and was seen 60% in low grade DCIS. There was significant difference statistically between groups for all of
these overexpressions (p<0,05).
Conclusions: P53 and HER/neu overexpressions were associated with poor prognostic factors such as comedo type
and high grade. Strong cytoplasmic bcl-2 staining was associated with good prognostic factors such as non-comedo
type and low grade. These results contribute to define DCIS subtypes that have different biological behaviour.

Keywords

Ductal carcinoma in situ, breast cancer, p53, HER2/neu, bcl-2

Memenin Duktal Karsinoma in Situ Lezyonları: Histopatolojik özellikler ile p53, HER2/neu, bcl-2 ve PCNA Ekspresyonu arasındaki ilişki

Abstract

Amaç:
Histopatolojik alt tip, nükleer derece ve komedonekroz memenin duktal karsinoma
in situ (DKIS) lezyonları için önemli özelliklerdir. Bu çalışmanın amacı
DKIS’nin histopatolojik özellikleri ile p53, HER2/neu, bcl-2 ve PCNA
overekspresyonu arasındaki ilişkinin belirlenmesidir. 

Yöntemler:
DKIS tanısı almış 40 olgu immünohistokimyasal metodlar kullanılarak p53,
HER2/neu, bcl-2 ve PCNA overekspresyonu açısından değerlendirildi. Düşük/
intermediate dereceli lezyonlar grup 1’e (düşük dereceli DKIS), yüksek dereceli
olgular grup 2’ye (yüksek dereceli DKIS) dahil edildi. Ayrıca histolojik
paternler değerlendirildi. Komedo tip ve non-komedo tip DKIS grupları
oluşturuldu. 

Bulgular:
Toplam 40 olgunun 12’si yüksek dereceli, 28’i düşük dereceli, 8’i komedo tip ve
32’si non-komedo tip DKIS olarak belirlendi. Komedo tip DKIS olgularında
sırasıyla %37,5, %62,5’inde p53 ve HER2/neu overekspresyonu izlenirken,
non-komedo tip DKIS olgularında bu oran % 6,5, %3,1 idi. Yüksek dereceli
olgularda sırasıyla %41,7, %50’sinde p53 ve HER2/neu overekspresyonu
izlenirken, düşük dereceli olguların hiçbirinde p53 ve HER2/neu overekspresyonu
gözlenmedi. Bcl-2 ile kuvvetli sitoplazmik boyanma yüksek dereceli olguların
hiçbirinde izlenmezken, düşük dereceli olguların %60’ında izlendi. Tüm bu
overekspresyonlar için gruplar arasında istatistiksel olarak anlamlı fark vardı
(p<0,05). 

Sonuçlar:
P53 ve HER2/neu overekspresyonu komedo tip ve yüksek derece gibi kötü
prognostik faktörlerle ilişkili bulundu. Bcl-2 ile kuvvetli sitoplazmik boyanma
non-komedo tip ve düşük derece gibi iyi prognostik faktörlerle ilişkili
bulundu. Bu sonuçlar farklı biyolojik davranışa sahip DKIS alt tiplerinin
tanımlanmasına katkı sağlayacaktır. 

Keywords

Duktal karsinoma in situ, meme kanseri, p53, HER2/neu

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