Determining the effect of an electromagnetic field generated by a high voltage power line on rat spermatogonia cells

Mehmet Cihan Yavaş; Veysi Akpolat; Engin Deveci; Hakki Murat Bilgin; İbrahim Kaplan; Ugur Seker; İsmail Yildiz; Esref Alkis; Mustafa Salih Celik; Mehmet Zülküf Akdağ

doi:10.5798/dicletip.497923

Determining the effect of an electromagnetic field generated by a high voltage power line on rat spermatogonia cells

Abstract

Objective: Purpose of the study was to research the effect of an electromagnetic field created by a high voltage line on the testes tissue and the serum biochemistry of Wistar albino male rats. At the same time, in the study also examined the protective effects of melatonin and ganoderma lucidum.

Methods: In the study, 64 rats were divided into eight equal groups (n: 8). In experiments lasting 26 and 52 days, the following groups were used: High voltage (HV), HV + Ganoderma lucidum (GI), HV + Melatonin (MEL), and Sham-control. MEL (10 mg/kg) was administered intraperitoneally and GI (20 mg/kg) as oral lavage. Extremely low frequency electric (80.3 V/m) and magnetic fields (2.48 µT) were applied for eight hours per day.

Results: The biochemical results of the study show an increase in total oxidant status (TOS) and oxidative stress index (OSI) level and a decrease in total antioxidant status (TAS) level in the 26 and 52 day high voltage groups, compared to the control group. In the high voltage groups, the nitric oxide (NO) value increased with the increase of the exposure period. Testicular mass (p>0.05), TAS, TOS, OSI and NO (p<0.05) were found at 26 days of comparison. Testicular weight, TAS, TOS, OSI (p<0.05), and NO (p>0.05) were found in 52 day comparisons. In routine histopathology data, we detected effects on both the 26 and 52 day HV groups, such as degeneration in spermatic cells and full structural deterioration and increasing hyalinisation. We also detected an step up in the ligament cell in the tubular field for the 52-day test groups. In melatonin added group, proliferating cell nuclear antigen (PCNA)-positive cells were more active. TUNEL assay analysis showed that there was no significant difference between the the 26 day experiment groups (p>0.05), but that there was a significant dissimilarity between the 52 day experiment groups (p<0.05).

Conclusions: In our study, the oxidative effect of created by a high voltage results in changes in serum biochemistry of rats and a number of degenerative deterioration in the histological structure of testes. Aslo, it is shown that the oxidative effect created by a high voltage has negative effects on the creation of spermatogonia. GI partly protects against these effects, while melatonin is more effective in this regard.

Keywords

Melatonin,ganoderma,high voltage,electromagnetic field,spermatogonia,antioxidant

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Details

Primary Language

Turkish

Subjects

-

Journal Section

Research Article

Authors

Mehmet Cihan Yavaş ^*
0000-0002-2923-050X
Türkiye

Veysi Akpolat This is me
0000-0002-2435-7800

Engin Deveci This is me
0000-0002-2353-1184
Türkiye

Hakki Murat Bilgin This is me
0000-0002-6040-9989
Türkiye

İbrahim Kaplan This is me
0000-0003-2813-1064
Türkiye

Ugur Seker
0000-0002-1693-6378
Türkiye

İsmail Yildiz This is me
0000-0001-5505-838X
Türkiye

Esref Alkis This is me
0000-0002-3321-2873
Türkiye

Mustafa Salih Celik This is me
0000-0001-7397-8120
Türkiye

Mehmet Zülküf Akdağ This is me
0000-0003-2826-2734
Türkiye

Publication Date

December 13, 2018

Submission Date

December 16, 2018

Acceptance Date

September 27, 2018

Published in Issue

Year 2018 Volume: 45 Number: 4

DOI

https://doi.org/10.5798/dicletip.497923

IZ

https://izlik.org/JA64SL83PJ

Cite

RIS / Bibtex

APA

Yavaş, M. C., Akpolat, V., Deveci, E., Bilgin, H. M., Kaplan, İ., Seker, U., Yildiz, İ., Alkis, E., Celik, M. S., & Akdağ, M. Z. (2018). Determining the effect of an electromagnetic field generated by a high voltage power line on rat spermatogonia cells. Dicle Medical Journal, 45(4), 447-461. https://doi.org/10.5798/dicletip.497923

AMA

1.Yavaş MC, Akpolat V, Deveci E, et al. Determining the effect of an electromagnetic field generated by a high voltage power line on rat spermatogonia cells. Dicle Medical Journal. 2018;45(4):447-461. doi:10.5798/dicletip.497923

Chicago

Yavaş, Mehmet Cihan, Veysi Akpolat, Engin Deveci, et al. 2018. “Determining the Effect of an Electromagnetic Field Generated by a High Voltage Power Line on Rat Spermatogonia Cells”. Dicle Medical Journal 45 (4): 447-61. https://doi.org/10.5798/dicletip.497923.

EndNote

Yavaş MC, Akpolat V, Deveci E, Bilgin HM, Kaplan İ, Seker U, Yildiz İ, Alkis E, Celik MS, Akdağ MZ (December 1, 2018) Determining the effect of an electromagnetic field generated by a high voltage power line on rat spermatogonia cells. Dicle Medical Journal 45 4 447–461.

IEEE

[1]M. C. Yavaş et al., “Determining the effect of an electromagnetic field generated by a high voltage power line on rat spermatogonia cells”, Dicle Medical Journal, vol. 45, no. 4, pp. 447–461, Dec. 2018, doi: 10.5798/dicletip.497923.

ISNAD

Yavaş, Mehmet Cihan - Akpolat, Veysi - Deveci, Engin - Bilgin, Hakki Murat - Kaplan, İbrahim - Seker, Ugur - Yildiz, İsmail - Alkis, Esref - Celik, Mustafa Salih - Akdağ, Mehmet Zülküf. “Determining the Effect of an Electromagnetic Field Generated by a High Voltage Power Line on Rat Spermatogonia Cells”. Dicle Medical Journal 45/4 (December 1, 2018): 447-461. https://doi.org/10.5798/dicletip.497923.

JAMA

1.Yavaş MC, Akpolat V, Deveci E, Bilgin HM, Kaplan İ, Seker U, Yildiz İ, Alkis E, Celik MS, Akdağ MZ. Determining the effect of an electromagnetic field generated by a high voltage power line on rat spermatogonia cells. Dicle Medical Journal. 2018;45:447–461.

MLA

Yavaş, Mehmet Cihan, et al. “Determining the Effect of an Electromagnetic Field Generated by a High Voltage Power Line on Rat Spermatogonia Cells”. Dicle Medical Journal, vol. 45, no. 4, Dec. 2018, pp. 447-61, doi:10.5798/dicletip.497923.

Vancouver

1.Mehmet Cihan Yavaş, Veysi Akpolat, Engin Deveci, Hakki Murat Bilgin, İbrahim Kaplan, Ugur Seker, İsmail Yildiz, Esref Alkis, Mustafa Salih Celik, Mehmet Zülküf Akdağ. Determining the effect of an electromagnetic field generated by a high voltage power line on rat spermatogonia cells. Dicle Medical Journal. 2018 Dec. 1;45(4):447-61. doi:10.5798/dicletip.497923

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