Thymoquinone induces apoptosis via targeting the Bax/BAD and Bcl-2 pathway in breast cancer cells

İbrahim Halil Yıldırım; Ali Ahmed Azzawri; Tuğçe Duran

doi:10.5798/dicletip.620329

Thymoquinone induces apoptosis via targeting the Bax/BAD and Bcl-2 pathway in breast cancer cells

Abstract

Objective: Nigella sativa, commonly known as the black seed, has been used since ancient times in folk medicine, and its various therapeutic benefits have been mentioned in some ancient medical sources and confirmed by modern science. Thymoquinone is the major compound and essential active ingredient of Nigella sativa seed oil. Thymoquinone has been reported to have high biological activity and broad therapeutic potential through several mechanisms that affect cells including anti-oxidant, anti-inflammatory, and anti-tumor properties. Although it has been widely reported that Thymoquinone inhibits cell growth and proliferation and stimulates apoptosis in various types of cancer cells, the mechanisms and signaling pathways are not fully understood. The aim of this study was to determine the effect of Thymoquinone on apoptosis in both tumor and non-tumor cells.

Methods: In this study, breast cancer cell line (MCF-7) was used as tumor cells and Human Embryonic Kidney Cell line (HEK293) was used as non-tumor cells. Cells were treated with Thymoquinone and viability test performed by MTT assay.Gene expression of apoptosis markers such as Bax, BAD, Bcl-2, and p53 was determined by Real-Time PCR. HEK293 cells were used as non-tumor control.

Results and Conclusion: Results suggest that Thymoquinone has a strong effect on cell proliferation and vitality. Thymoquinone has increased the expression of BAD, Bax genes which induce apoptosis and decreased the p53 gene in breast cancer cells. Therefore Thymoquinone promotes apoptosis and enhances anti-cancer efficacy in breast cancer cells.

Keywords

Thymoquinone,breast cancer

References

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Details

Primary Language

English

Subjects

Health Care Administration

Journal Section

Research Article

Authors

İbrahim Halil Yıldırım ^*
0000-0001-5518-5004

Ali Ahmed Azzawri This is me
0000-0001-5311-8887

Tuğçe Duran This is me
0000-0002-7353-4527

Publication Date

September 16, 2019

Submission Date

December 24, 2018

Acceptance Date

March 7, 2019

Published in Issue

Year 2019 Volume: 46 Number: 3

DOI

https://doi.org/10.5798/dicletip.620329

IZ

https://izlik.org/JA26LM92HS

Cite

RIS / Bibtex

APA

Yıldırım, İ. H., Azzawri, A. A., & Duran, T. (2019). Thymoquinone induces apoptosis via targeting the Bax/BAD and Bcl-2 pathway in breast cancer cells. Dicle Medical Journal, 46(3), 411-417. https://doi.org/10.5798/dicletip.620329

AMA

1.Yıldırım İH, Azzawri AA, Duran T. Thymoquinone induces apoptosis via targeting the Bax/BAD and Bcl-2 pathway in breast cancer cells. Dicle Medical Journal. 2019;46(3):411-417. doi:10.5798/dicletip.620329

Chicago

Yıldırım, İbrahim Halil, Ali Ahmed Azzawri, and Tuğçe Duran. 2019. “Thymoquinone Induces Apoptosis via Targeting the Bax BAD and Bcl-2 Pathway in Breast Cancer Cells”. Dicle Medical Journal 46 (3): 411-17. https://doi.org/10.5798/dicletip.620329.

EndNote

Yıldırım İH, Azzawri AA, Duran T (September 1, 2019) Thymoquinone induces apoptosis via targeting the Bax/BAD and Bcl-2 pathway in breast cancer cells. Dicle Medical Journal 46 3 411–417.

IEEE

[1]İ. H. Yıldırım, A. A. Azzawri, and T. Duran, “Thymoquinone induces apoptosis via targeting the Bax/BAD and Bcl-2 pathway in breast cancer cells”, Dicle Medical Journal, vol. 46, no. 3, pp. 411–417, Sept. 2019, doi: 10.5798/dicletip.620329.

ISNAD

Yıldırım, İbrahim Halil - Azzawri, Ali Ahmed - Duran, Tuğçe. “Thymoquinone Induces Apoptosis via Targeting the Bax BAD and Bcl-2 Pathway in Breast Cancer Cells”. Dicle Medical Journal 46/3 (September 1, 2019): 411-417. https://doi.org/10.5798/dicletip.620329.

JAMA

1.Yıldırım İH, Azzawri AA, Duran T. Thymoquinone induces apoptosis via targeting the Bax/BAD and Bcl-2 pathway in breast cancer cells. Dicle Medical Journal. 2019;46:411–417.

MLA

Yıldırım, İbrahim Halil, et al. “Thymoquinone Induces Apoptosis via Targeting the Bax BAD and Bcl-2 Pathway in Breast Cancer Cells”. Dicle Medical Journal, vol. 46, no. 3, Sept. 2019, pp. 411-7, doi:10.5798/dicletip.620329.

Vancouver

1.İbrahim Halil Yıldırım, Ali Ahmed Azzawri, Tuğçe Duran. Thymoquinone induces apoptosis via targeting the Bax/BAD and Bcl-2 pathway in breast cancer cells. Dicle Medical Journal. 2019 Sep. 1;46(3):411-7. doi:10.5798/dicletip.620329

Thymoquinone ameliorate oxidative stress, GABAergic neuronal depletion and memory impairment through Nrf2/ARE signaling pathway in the dentate gyrus following cypermethrin administration

BMC Neuroscience

https://doi.org/10.1186/s12868-024-00896-7

Thymoquinone induces apoptosis via targeting the Bax/BAD and Bcl-2 pathway in breast cancer cells

Thymoquinone induces apoptosis via targeting the Bax/BAD and Bcl-2 pathway in breast cancer cells

Abstract

Keywords

References

Details

Primary Language

Subjects

Journal Section

Authors

Publication Date

Submission Date

Acceptance Date

Published in Issue

DOI

IZ

Cite

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