Effects of sildenafil on isoniazid and streptomycin neurotoxicity in rats

Abstract

Objectives: Neurotoxicity due to isoniazide (INH) and streptomycin has been reported. Experimental studies reported that sildenafil can reduce toxic effects of some drugs. The aim of this study was to investigate the pos­sible toxic effects of long-term streptomycin and INH use and probable protective effects of sildenafil on the rat brain and cerebellum. Materials and methods: In this study, 78 rats were di­vided into eight groups as follows; control group (n=10), INH group (n=10), streptomycin group (n=10), INH + streptomycin group (n=10), sildenafil group (n=10), INH+sildenafil group (n=10), streptomycin+sildenafil group (n=8) and INH+streptomycin + sildenafil group (n=10). At the end of 30 days of the treatment, the brain and cerebellar tissues of the rats were examined histo­pathologically. On the all tissues, inflammation, edema, congestion, degeneration and necrosis were investigated under a light microscope by the same pathologist blinded to the experimental groups. Results: Degeneration was significantly more prominent in the streptomycin group compared with the control group (respectively, p=0.002, p

Keywords

• Isoniazid, streptomycin, sildenafil, neurotox­icity, histopathology

Sıçanlarda izoniazid ve streptomisin nörotoksisitesine sildenafil\'in etkileri

Abstract

Amaç: İzoniazid (İNH) ve streptomisin (STR) kombinas­yonuna bağlı nörotoksisite bildirilmiştir. Sildenafil\'in (SLD) deneysel olarak bazı ilaç toksisitelerini azaltabildiği öne sürülmüştür. Çalışmada amacımız; uzun süreli İNH ve STR verilen sıçanların beyninde ve serebellumunda tok­sik etki olup olmadığı ve olası bu toksisite üzerine SLD\'nin etkisini araştırmaktır. Gereç ve yöntem: Bu çalışmada 78 sıçan; kontrol grubu (n= 10), İNH grubu (n= 10), STR grubu (n= 10), İNH + STR - (n= 10), SLD (n= 10), İNH + SLD (n= 10), STR + SLD (n= 8), İNH + STR + SLD (n= 10) grupları ola­rak sekiz gruba ayrıldı. İlaçlar 30 gün boyunca uygulan­dı. Sıçanların beyin ve serebellumu histopatolojik olarak incelendi. Beyin ve serebellum dokusunda; inflamasyon, ödem, konjesyon, dejenerasyon ve nekroz bulguları araş­tırıldı. Histopatolojik değerlendirme çalışma gruplarından habersiz tek bir patolog tarafından değerlendirildi. Bulgular: STR grubu sıçan beyninde ve serebellumunda kontrol grubuna göre istatistiksel olarak anlamlı yüksek dejenerasyon bulundu (sırayla, p=0.002, p

Keywords

• İzoniazid, streptomisin, sildenafil, nö­rotoksisite, histopatoloji

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