Fusidic acid resistance among staphylococci strains isolated from clinical specimens in a general hospital

Abstract

Objectives: The aim of this study was to investigate in vitro susceptibility of fusidic acid to clinic isolates of staphylo-cocci. Materials and methods: The forty-one coagulase negative staphylococci (CNS) and 18 Staphylococcus aureus strains isolated from various clinical specimens were included in this study. Staphylococci isolates were identified by conventional methods such as colony morphology onto medium, gram staining, catalase and coagulase tests. Ac-cording to “Clinical and Laboratory Standards Institute (CLSI)” criteria, antimicrobial susceptibility testing of isolates was performed by Kirby-Bauer\'s disk diffusion method. Results: The seventy-two percent of the isolated S.aureus were defined as methicillin sensitive-S.aureus (MSSA), 28% of the isolated S.aureus were defined as methicillin resistant-S.aureus (MRSA). The difference among fusidic acid susceptibility rates of MSSA and MRSA strains was not statistically significant (p=0.305). The twenty-nine per-cent of the isolated CNS were defined as methicillin sensitive-CNS (MS-CNS), 71% of the isolated CNS were defined as methicillin resistant-CNS (MR-CNS). There was no statistically significant difference between MS-CNS and MR-CNS strains for fusidic acid susceptibility rates (p=0.490). But the difference among fusidic acid susceptibility rates of CNS and S.aureus strains was statistically significant (p

Keywords

• Staphylococcus aureus, fusidic acid, microbial sensitivity test

Fusidic acid resistance among staphylococci strains isolated from clinical specimens in a general hospital

Abstract

Amaç: Bu çalışmanın amacı, klinik örneklerden izole edilen stafilokok suşlarında fusidik asidin in vitro etkinliğinin araştırılmasıdır. Gereç ve yöntem: Çalışmaya çeşitli klinik örneklerden izole edilen 41 koagülaz negatif stafilokok (KNS) izolatı ile 18 Staphylococcus aureus suşu dahil edildi. Stafilokok izolatları besiyeri yüzeyindeki koloni morfolojisi, gram boyama, katalaz ve koagülaz testleri gibi konvansiyonel yöntemlerle identifiye edildi. İzolatların antimikrobiyal duyarlılıkları “Clinical and Laboratory Standards Institute (CLSI)” önerileri doğrultusunda Kirby-Bauer disk difüzyon yöntemi kulla-nılarak çalışıldı. Bulgular: İzole edilen S.aureus suşlarının % 72\'si metisiline duyarlı (MSSA), % 28\'i metisiline dirençli (MRSA) olarak tanımlandı. MSSA ve MRSA suşlarının fusidik asit duyarlılık oranları arasındaki fark istatistiksel olarak anlamlı bu-lunmadı (p=0.305). İzole edilen KNS\'lerin % 29\'u metisiline duyarlı (MS-KNS), % 71\'i metisiline dirençli (MR-KNS) olarak tanımlandı. MR-KNS ve MS-KNS suşlarının fusidik asit duyarlılık oranları arasında istatistiksel olarak anlamlı fark yoktu (p=0.490). Ancak, KNS ve S.aureus suşlarının fusidik asit duyarlılık oranları arasındaki fark istatistiksel ola-rak anlamlıydı (p

Keywords

• Staphylococcus aureus, fusidik asit, mikrobiyal duyarlılık testi

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