Comparison of VITEK 2 YST Card and API 20C AUX system in identification of non- albicans Candida species

Abstract

Objectives: In the present study, it was aimed to compare results obtained by using VITEK 2 YST Card (bioMérieux, France) with those obtained by using API 20C AUX (bioMérieux, France) for identification of non- albicans Candida species, which was isolated from various clinical samples, at level of species. Materials and methods: Forty-one non-albicans Candida isolates, which were isolated from 28 urine, 10 blood and 3 vaginal swab specimens, and found to be negative by germ tube test, were identified by using VITEK 2 YST Card (bioMérieux, France). In addition, microscopic morphology was assessed in corn-meal Tween 80 agar, while carbo-hydrate assimilation was assessed by using commercially available API 20C AUX kit (bioMérieux, France). Results: Thirty-four isolates (82.9%) were identified as identical species by these 2 systems, while different results were obtained in 7 isolates (17.1%). 5 isolates, identified as Candida glabrata by API 20C AUX system, were identi-fied as Candida tropicalis (n=2), Candida krusei, Candida lipolitica and Candida kefyr by VITEK 2 YST Card. One other isolate, identified as C.tropicalis, was identified as Candida parapsilosis; and additional one isolate, identified as C.parapsilosis, was identified as C.tropicalis. Conclusion: It was concluded that one should be cautious in the identification of C.glabrata, in particular, C.tropicalis and C.parapsilosis, although between VITEK 2 YST Card and API 20C AUX system results was found largely similar-ity in identification of non-albicans Candida spp.

Keywords

• Non-albicans Candida spp., identification, phenotypic systems.

Comparison of VITEK 2 YST Card and API 20C AUX system in identification of non- albicans Candida species

Abstract

Amaç: Bu çalışmada çeşitli klinik örneklerden soyutlanan Candida albicans dışı mayaların tür düzeyinde tanımlan-masında VITEK 2 YST kart (bioMérieux, Fransa) kullanılarak elde edilen sonuçların API 20C AUX (bioMérieux, Fran-sa) sistemi ile karşılaştırılması amaçlandı. Gereç ve yöntem: Yirmi sekiz idrar, 10 kan, 3 vajinal akıntı örneğinden soyutlanan ve germ tüp testi negatif olarak bulunan 41 C.albicans dışı maya izolatı VITEK 2 YST kart (bioMérieux, Fransa) ile tanımlandı. Ayrıca mısır unu-Tween 80 agardaki mikroskobik morfoloji ve API 20C AUX (bioMérieux, Fransa) ticari kiti ile yapılan karbonhidrat asimilasyonu değerlendirildi. Bulgular: Her iki sistemle izolatların 34\'ü (%82.9) aynı tür olarak tanımlanırken, 7\'sinde (%17.1) farklı sonuçlar elde edildi. API 20C AUX sistemi ile Candida glabrata olarak tanımlanan 5 izolat VITEK 2 YST kart ile Candida tropicalis (n:2), Candida krusei, Candida lipolitica ve Candida kefyr olarak, C.tropicalis olarak tanımlanan bir başka izolat Can-dida parapsilosis olarak, C.parapsilosis olarak tanımlanan bir diğer izolat ise C.tropicalis olarak tanımlandı. Sonuç: VITEK 2 YST kart sistemi ile API 20C AUX sistemi arasında C.albicans dışı mayaların tür düzeyinde tanım-lanmasında büyük oranda benzerlik bulunmasına karşın, özellikle C.glabrata, başta olmak üzere C.tropicalis ve C.parapsilosis türlerinin tanımlanmasında dikkatli olunması gerektiği kanaatine varıldı.

Keywords

• C.albicans dışı maya, tanımlama, fenotipik sistemler.

References

1. Tümbay E. Candida, Cryptococcus ve Tıbbi Önemi Olan Di- ğer Mayalar. Çev ed. Başustaoğlu A. Klinik Mikrobiyoloji, 9. Baskı, Ankara: Atlas kitapçılık, 2009: 1762-1788.
2. Tümbay E. Candida Türleri. Ustaçelebi Ş ed. Temel ve Klinik Mikrobiyoloji. Ankara: Güneş Kitabevi, 1999: 1081-1086.
3. Hata DJ, Hall L, Fothergill AW, Larone DH, Wengenack NL. Multicenter evaluation of the new VITEK 2 advanced colorimetric yeast identification card. J Clin Microbiol 2007;45(4):1087-92.
4. Aubertine CL, Rivera M, Rohan SM, Larone DH. Compara- tive study of the new colorimetric VITEK 2 yeast iden- tification card versus the older fluorometric card and of CHROMagar Candida as a source medium with the new card. J Clin Microbiol 2006;44(1):227-8.
5. Graf B, Adam T, Zill E, Göbel UB. Evaluation of the VITEK 2 system for rapid identification of yeasts and yeast-like or- ganisms. J Clin Microbiol 2000;38(5):1782-5.
6. Lin CC, Fung DY. Conventional and rapid methods for yeast identification. Crit Rev Microbiol 1987;14(4):273-89.
7. Liguori G, Gallé F, Lucariello A, et al. Comparison between multiplex PCR and phenotypic systems for Candida spp. identification. New Microbiol 2010;33(1):63-7.
8. Valenza G, Strasen J, Schäfer F, Frosch M, Kurzai O, Abele- Horn M. Evaluation of new colorimetric vitek 2 yeast iden- tification card by use of different source media. J Clin Mi- crobiol 2008;46(11): 3784-7.