Investigation of Pepsin in Laryngeal Squamous Cell Carcinoma Specimens

Hamdi Tasli; Burcu Eser; Hakan Birkent; Burak Asik; Mustafa Gerek

doi:10.5798/dicletip.661237

Investigation of Pepsin in Laryngeal Squamous Cell Carcinoma Specimens

Abstract

Objective: Squamous cell carcinoma (SCC) composes 95% of the carcinoma of the larynx. One of the factor recognized in the past two decades as increasing the risk of SCC is laryngopharyngeal reflux (LPR). The role and mechanism of LPR in laryngeal cancer remain unclear. The aim of this study was to assess the presence of pepsin in laryngeal formalin-fixed, paraffin-embedded (FFPE) squamous cell carcinoma (SCC) specimens in order to test the hypothesis that LPR plays a role in the development of laryngeal SCC. Method: A total of 29 pathology specimens of advanced-stage laryngeal cancer patients who had undergone total laryngectomy were evaluated. The specimens were confirmed histopathologically as SCC and maintained in paraffin blocks in the pathology department. The western blot (WB) or the enzyme-linked immunosorbent assay (ELISA) analyses were used to measure pepsin enzyme levels. Results: According to the WB and ELISA analyses, there was no presence of the pepsin enzyme in any of the 29 formalin-fixed, paraffin-embedded (FFPE) laryngeal SCC specimens. However, pepsin was detected with both the WB and the ELISA tests in positive controls and fresh rat stomach specimen. Conclusion: Pepsin was not present in any of the malignant laryngeal squamous cell carcinoma specimens in this study. Relationship between LPR and laryngeal SCC still remains unclear and further studies are needed to verify the role of pepsin and LPR in laryngeal SCC.

Keywords

Laryngopharyngeal reflux; laryngeal carcinoma; pepsin; larynx

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Details

Primary Language

English

Subjects

Health Care Administration

Journal Section

Research Article

Authors

Hamdi Tasli ^* This is me
0000-0003-2186-5006
Türkiye

Burcu Eser This is me
0000-0001-5641-3734
Türkiye

Hakan Birkent This is me
0000-0001-9545-6518
Türkiye

Burak Asik This is me
Türkiye

Mustafa Gerek This is me
Türkiye

Publication Date

December 15, 2019

Submission Date

April 3, 2019

Acceptance Date

September 23, 2019

Published in Issue

Year 2019 Volume: 46 Number: 4

DOI

https://doi.org/10.5798/dicletip.661237

IZ

https://izlik.org/JA72GF96MG

Cite

RIS / Bibtex

APA

Tasli, H., Eser, B., Birkent, H., Asik, B., & Gerek, M. (2019). Investigation of Pepsin in Laryngeal Squamous Cell Carcinoma Specimens. Dicle Medical Journal, 46(4), 657-665. https://doi.org/10.5798/dicletip.661237

AMA

1.Tasli H, Eser B, Birkent H, Asik B, Gerek M. Investigation of Pepsin in Laryngeal Squamous Cell Carcinoma Specimens. Dicle Medical Journal. 2019;46(4):657-665. doi:10.5798/dicletip.661237

Chicago

Tasli, Hamdi, Burcu Eser, Hakan Birkent, Burak Asik, and Mustafa Gerek. 2019. “Investigation of Pepsin in Laryngeal Squamous Cell Carcinoma Specimens”. Dicle Medical Journal 46 (4): 657-65. https://doi.org/10.5798/dicletip.661237.

EndNote

Tasli H, Eser B, Birkent H, Asik B, Gerek M (December 1, 2019) Investigation of Pepsin in Laryngeal Squamous Cell Carcinoma Specimens. Dicle Medical Journal 46 4 657–665.

IEEE

[1]H. Tasli, B. Eser, H. Birkent, B. Asik, and M. Gerek, “Investigation of Pepsin in Laryngeal Squamous Cell Carcinoma Specimens”, Dicle Medical Journal, vol. 46, no. 4, pp. 657–665, Dec. 2019, doi: 10.5798/dicletip.661237.

ISNAD

Tasli, Hamdi - Eser, Burcu - Birkent, Hakan - Asik, Burak - Gerek, Mustafa. “Investigation of Pepsin in Laryngeal Squamous Cell Carcinoma Specimens”. Dicle Medical Journal 46/4 (December 1, 2019): 657-665. https://doi.org/10.5798/dicletip.661237.

JAMA

1.Tasli H, Eser B, Birkent H, Asik B, Gerek M. Investigation of Pepsin in Laryngeal Squamous Cell Carcinoma Specimens. Dicle Medical Journal. 2019;46:657–665.

MLA

Tasli, Hamdi, et al. “Investigation of Pepsin in Laryngeal Squamous Cell Carcinoma Specimens”. Dicle Medical Journal, vol. 46, no. 4, Dec. 2019, pp. 657-65, doi:10.5798/dicletip.661237.

Vancouver

1.Hamdi Tasli, Burcu Eser, Hakan Birkent, Burak Asik, Mustafa Gerek. Investigation of Pepsin in Laryngeal Squamous Cell Carcinoma Specimens. Dicle Medical Journal. 2019 Dec. 1;46(4):657-65. doi:10.5798/dicletip.661237