Research Article
BibTex RIS Cite

A Comparison Of Manual Hemocytometry And Different Modes Of Mindray BC-6800 Hematology Analyzer For Cell Count In Peritoneal And Pleural Fluids

Year 2020, , 278 - 285, 17.06.2020
https://doi.org/10.5798/dicletip.755702

Abstract

Objective: Total white blood cell (WBC) count in body fluids (BF) is important in diagnosis and treatment of inflammatory and infectious diseases. We aimed to compare hemogram and BF modes of Mindray BC-6800 hematology analyzer with the manual hemocytometry method for determination of total WBC count in peritoneal and pleural fluids.
Method: Our study consisted of a total of 143 specimens, 109 peritoneal fluid and 34 pleural effusion fluid. Each sample was analyzed consecutively twice, first with the manual method and, later with both hemogram mode and the BF mode of Mindray BC-6800 automated hematology analyzer.
Results: There was a statistically significant difference between manual count and hemogram mode (for peritoneal fluids p< 0.001, for pleural fluids p< 0.001). There was a statistically significant difference between the hemogram mode and BF mode too. (for peritoneal fluids p< 0.001, for pleural fluids p< 0.001). However, there was no statistically significant difference between the manual count and BF mode (for peritoneal fluids p=0.236, for pleural fluids p=0.627). Less than100 cells/mL, there was a statistically significant difference between each of the counting methods (Manual count vs hemogram mode p< 0.001, manual count vs BF mode p= 0.012, hemogram mode vs BF mode p< 0.001).
More than 100 cells/mL, there was no statistically significant difference between manual count and the BF mode (p= 0.332). However, there were statistically significant differences between manual count and hemogram mode, and hemogram mode and BF mode (p= 0.003 and p< 0.001 respectively).
Conclusion: The use of manual method instead of hematology analyzer is more convenient in samples <100 cells/mL, and BF mode may be used as a screening tool in samples >100 cells/mL.

References

  • 1. De Smet D, Van Moer G, Martens GA, et al. Use of the Cell-Dyn Sapphire hematology analyzer for automated counting of blood cells in body fluids. Am J Clin Pathol. 2010; 133: 291-9.
  • 2. Cognialli RCR, Comar SR, Souza AMD, Singer GMB. Evaluation of pleural and ascitic fluid analysis on the Sysmex XE-5000 hematology analyzer. J Bras Patol Med Lab. 2017; 53; 150-8.
  • 3. Fuster O, Andino B, Pardo A, Laiz B. Continuous ambulatory peritoneal dialysis, ascitic and pleural body fluids evaluation with the Mindray BC-6800 hematology analyzer. Journal of clinical laboratory analysis. J Clin Lab Anal. 2018; 32: e22240.
  • 4. Runyon BA. Management of adult patients with ascites due to cirrhosis: an update. Hepatology. 2009; 49: 2087-107.
  • 5. Conner BD, Lee YG, Branca P, Rogers JT, Rodriguez RM, Light RW. Variations in pleural fluid WBC count and differential counts with different sample containers and different methods. Chest. 2003; 123: 1181-7.
  • 6. Bottini PV, Garlipp CR, Nogueira BF, Pompeo DB, Souza MI (2018). Performance evaluation of a new generation of automated analyzer for pleural and peritoneal fluids cytology. Clin Lab. 2018; 64: 1783-6.
  • 7. Schonenberg H, Fassl H. Comparison of the coulter-counter-method and the counting-chamber to determine the cell count of the cerebrospinal fluid. Arztliche Forschung. 1970; 24: 118.
  • 8. Walker TJ, Nelson LD, Dunphy BW, Anderson DM, Kickler TS. Comparative Evaluation of the Iris iQ200 Body Fluid Module With Manual Hemacytometer Count. Am J Clin Pathol. 2009; 131: 333-8.
  • 9. Talstad I. Electronic counting of spinal fluid cells. Am J Clin Pathol. 1984; 81: 506-11.
  • 10. Aune MW, Becker JL, Brugnara C, et al. Automated flow cytometric analysis of blood cells in cerebrospinal fluid. Am J Clin Pathol. 2004; 121: 690-700.
  • 11. Takemura H, Ai T, Kimura K, et al. Evaluation of cell count and classification capabilities in body fluids using a fully automated Sysmex XN equipped with high-sensitive Analysis (hsA) mode and DI-60 hematology analyzer system. PloS one. 2018; 13: e0195923.
  • 12. Li PKT, Szeto CC, Piraino B, et al. Peritoneal dialysis-related infections recommendations: 2010 update. Perit Dial Int. 2010; 30: 393-423.
  • 13. Fleming C, Russcher H, Lindemans J, de Jonge R. Clinical relevance and contemporary methods for counting blood cells in body fluids suspected of inflammatory disease. Clin Chem Lab Med. 2015; 53: 1689-706.
Year 2020, , 278 - 285, 17.06.2020
https://doi.org/10.5798/dicletip.755702

Abstract

References

  • 1. De Smet D, Van Moer G, Martens GA, et al. Use of the Cell-Dyn Sapphire hematology analyzer for automated counting of blood cells in body fluids. Am J Clin Pathol. 2010; 133: 291-9.
  • 2. Cognialli RCR, Comar SR, Souza AMD, Singer GMB. Evaluation of pleural and ascitic fluid analysis on the Sysmex XE-5000 hematology analyzer. J Bras Patol Med Lab. 2017; 53; 150-8.
  • 3. Fuster O, Andino B, Pardo A, Laiz B. Continuous ambulatory peritoneal dialysis, ascitic and pleural body fluids evaluation with the Mindray BC-6800 hematology analyzer. Journal of clinical laboratory analysis. J Clin Lab Anal. 2018; 32: e22240.
  • 4. Runyon BA. Management of adult patients with ascites due to cirrhosis: an update. Hepatology. 2009; 49: 2087-107.
  • 5. Conner BD, Lee YG, Branca P, Rogers JT, Rodriguez RM, Light RW. Variations in pleural fluid WBC count and differential counts with different sample containers and different methods. Chest. 2003; 123: 1181-7.
  • 6. Bottini PV, Garlipp CR, Nogueira BF, Pompeo DB, Souza MI (2018). Performance evaluation of a new generation of automated analyzer for pleural and peritoneal fluids cytology. Clin Lab. 2018; 64: 1783-6.
  • 7. Schonenberg H, Fassl H. Comparison of the coulter-counter-method and the counting-chamber to determine the cell count of the cerebrospinal fluid. Arztliche Forschung. 1970; 24: 118.
  • 8. Walker TJ, Nelson LD, Dunphy BW, Anderson DM, Kickler TS. Comparative Evaluation of the Iris iQ200 Body Fluid Module With Manual Hemacytometer Count. Am J Clin Pathol. 2009; 131: 333-8.
  • 9. Talstad I. Electronic counting of spinal fluid cells. Am J Clin Pathol. 1984; 81: 506-11.
  • 10. Aune MW, Becker JL, Brugnara C, et al. Automated flow cytometric analysis of blood cells in cerebrospinal fluid. Am J Clin Pathol. 2004; 121: 690-700.
  • 11. Takemura H, Ai T, Kimura K, et al. Evaluation of cell count and classification capabilities in body fluids using a fully automated Sysmex XN equipped with high-sensitive Analysis (hsA) mode and DI-60 hematology analyzer system. PloS one. 2018; 13: e0195923.
  • 12. Li PKT, Szeto CC, Piraino B, et al. Peritoneal dialysis-related infections recommendations: 2010 update. Perit Dial Int. 2010; 30: 393-423.
  • 13. Fleming C, Russcher H, Lindemans J, de Jonge R. Clinical relevance and contemporary methods for counting blood cells in body fluids suspected of inflammatory disease. Clin Chem Lab Med. 2015; 53: 1689-706.
There are 13 citations in total.

Details

Primary Language English
Subjects Health Care Administration
Journal Section Original Articles
Authors

Ünsal Savcı This is me

Mustafa Şahin This is me

Barış Eser This is me

Publication Date June 17, 2020
Submission Date September 24, 2019
Published in Issue Year 2020

Cite

APA Savcı, Ü., Şahin, M., & Eser, B. (2020). A Comparison Of Manual Hemocytometry And Different Modes Of Mindray BC-6800 Hematology Analyzer For Cell Count In Peritoneal And Pleural Fluids. Dicle Tıp Dergisi, 47(2), 278-285. https://doi.org/10.5798/dicletip.755702
AMA Savcı Ü, Şahin M, Eser B. A Comparison Of Manual Hemocytometry And Different Modes Of Mindray BC-6800 Hematology Analyzer For Cell Count In Peritoneal And Pleural Fluids. diclemedj. June 2020;47(2):278-285. doi:10.5798/dicletip.755702
Chicago Savcı, Ünsal, Mustafa Şahin, and Barış Eser. “A Comparison Of Manual Hemocytometry And Different Modes Of Mindray BC-6800 Hematology Analyzer For Cell Count In Peritoneal And Pleural Fluids”. Dicle Tıp Dergisi 47, no. 2 (June 2020): 278-85. https://doi.org/10.5798/dicletip.755702.
EndNote Savcı Ü, Şahin M, Eser B (June 1, 2020) A Comparison Of Manual Hemocytometry And Different Modes Of Mindray BC-6800 Hematology Analyzer For Cell Count In Peritoneal And Pleural Fluids. Dicle Tıp Dergisi 47 2 278–285.
IEEE Ü. Savcı, M. Şahin, and B. Eser, “A Comparison Of Manual Hemocytometry And Different Modes Of Mindray BC-6800 Hematology Analyzer For Cell Count In Peritoneal And Pleural Fluids”, diclemedj, vol. 47, no. 2, pp. 278–285, 2020, doi: 10.5798/dicletip.755702.
ISNAD Savcı, Ünsal et al. “A Comparison Of Manual Hemocytometry And Different Modes Of Mindray BC-6800 Hematology Analyzer For Cell Count In Peritoneal And Pleural Fluids”. Dicle Tıp Dergisi 47/2 (June 2020), 278-285. https://doi.org/10.5798/dicletip.755702.
JAMA Savcı Ü, Şahin M, Eser B. A Comparison Of Manual Hemocytometry And Different Modes Of Mindray BC-6800 Hematology Analyzer For Cell Count In Peritoneal And Pleural Fluids. diclemedj. 2020;47:278–285.
MLA Savcı, Ünsal et al. “A Comparison Of Manual Hemocytometry And Different Modes Of Mindray BC-6800 Hematology Analyzer For Cell Count In Peritoneal And Pleural Fluids”. Dicle Tıp Dergisi, vol. 47, no. 2, 2020, pp. 278-85, doi:10.5798/dicletip.755702.
Vancouver Savcı Ü, Şahin M, Eser B. A Comparison Of Manual Hemocytometry And Different Modes Of Mindray BC-6800 Hematology Analyzer For Cell Count In Peritoneal And Pleural Fluids. diclemedj. 2020;47(2):278-85.