The protective effects of acetyl L - carnitine which added into Histidine - Tryptophan - Ketoglutarate solution on donor uterus

Year 2016, Volume: 43 Issue: 4, 561 - 568, 25.12.2016

İlkay Demircan Candan Özoğul Seda Nur Akyol Mustafa Necmi İlhan Mustafa Kavutçu Süreyya Barun Mustafa Bilge İbrahim Murat Hirfanoğlu

https://doi.org/10.5798/diclemedj.0921.2015.01.0521

Abstract

Objective: At the present times uterus transplantation
is an alternative therapy for women with untreatable uterine-based
infertility factors. Before transplantation, the donor organ must stored
in some solutions, but they may not adequate for protection. In this
study, we investigated the potential protective effects of acetyl
L-carnitine, added into histidine-tryptophanketoglutarate (HTK)
solution, on rat uterus.

Methods: We divided 24 female Wistar Albino rats into
four groups (n=6). Their uterine tissues were stored into four different
solutions at different periods at 4oC ; Group 1 HTK, 4 hours; Group 2.
HTK + acetyl L- carnitine, 4 hours, Group 3. HTK, 24 hours; Group 4. HTK
+ acetyl L- carnitine, 24 hours. Then tissues from uterus were used for
histological and biochemical examination.

Results: In the study the number of TUNEL positive
cells in group 4 was lower than group 3. Biochemically evaluated TBARS
and NOS levels were highest in group 3 however CAT level was highest in
group 2.

Conclusion: In conclusion, addition of acetyl
L-carnitine to HTK solution reversed the histological alterations after
24 hours cold storage on rat uterus.

Keywords

acetyl L-carnitine, uterus, transplantation, infertility

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