Trombositten zengin Fibrinin periferik sinir iyileşmesi üzerindeki histopatolojik etkileri

Year 2017, Volume: 44 Issue: 3, 243 - 250, 20.09.2017

Hasan Metineren , Turan Cihan Dülgeroğlu Mehmet Hüseyin Metineren

https://doi.org/10.5798/dicletip.338997

Cited By: 2

Abstract

Amaç: Sinir iyileşmesini hızlandırmak ve kalitesini artırmak için çeşitli materyallerle deneysel çalışmalar yapılmıştır. Çalışmamızın amacı trombositten
zengin fibrinin (TZF) siyatik sinir iyileşmesi üzerindeki histopatolojik etkilerini
deneysel rat modelinde araştırmaktır.

Yöntemler: 16 Sprague Dawley rat randomize iki guruba (8 kontrol, 8 TZF) ayrıldı. Tüm ratların siyatik sinirleri
kesilip 2mm defektli olarak tekrar onarıldı. TZF gurubunda
defektif bölgeye TZF sarıldı. 6 haftanın sonunda ratlar
sakrifiye edilerek histolojik ve immüno-histokimyasal
metotlarla TZF nin kontrol gurubuna göre sinir onarımına
katkısı araştırıldı.

Sonuç: TZF gurubunun histolojik skorları, kontrol gurubuna göre istatistiksel olarak anlamlı bulundu(P=0.001). TZF sinirlerde aksonal devamlılık mevcuttu. Kontrol grubunda defektin tamamen iyileşmediği
yada
fibröz/granülasyon dokuyla iyileştiği gözlendi. Bol miktarda hücresel içerik ve büyüme
faktörü ihtiva eden TZF sinir iyileşmesine olumlu katkı sağlamaktadır.

Keywords

Trombositten zengin fibrin, sinir iyileşmesi, deneysel rat modeli

References

• 1. Noble J, Munro CA, Prasad VS, et al. Analysis of upper and lower extremity peripheral nerve injuries in a population of patients with multiple injuries. J Trauma1998; 45:116–22.
• 2. Choukroun J, Diss A, Simonpieri A, et al. Platelet-rich fibrin (PRF): a second-generation platelet. s.l.: concentrate. Part V: histologic evaluations of PRF effects on bone allograft maturation in sinus lift. Oral Surg Oral Med Oral Pathol Oral Radiol Endod 2006;101:299-303.
• 3. Weibrich G, Kleis WKG, Hafner G. Growth factor levels in platelet rich plasma and correlation with donor age, sex and platelet count. J Craniomaxillofac Surg 2002;30:97–102.
• 4. Dohan Ehrenfest DM, Rasmusson L, Albrektsson T. Classification of platelet concentrates from pure platelet-rich plasma (P-PRP) to leucocyte and platelet- rich fibrin (L-PRF). Trends in Biotechnology 2009;27:158-67.
• 5. Sean M. O’Connell, Theresa Impeduglia, Karen Hessler, et al. Autologous platelet-rich fibrin matrix as cell therapy in the healing of chronic lower-extremity ulcers. Wound Repair and Regeneration 2008;16:749-56.
• 6. Klopfleisch R. Multiparametric and semiquantitative scoring systems for the evaluation of mouse model histopathology—a systematic review. BMC Vet Res 2013; 9:123.
• 7. Giddins GE, Wade PJ, Amis AA. Primary nerve repair: strength of repair with different gauges of nylon suture material. J Hand Surg Br. 1989; 14:301-2.
• 8. Panagopoulos GN, Megaloikonomos PD, Mavrogenis AF. The Present and Future for Peripheral Nerve Regeneration. Orthopedics. 2016; 25:1-16.
• 9. Sariguney Y, Yavuzer R, Elmas C. Effect of platelet-rich plasma on peripheral nerve regeneration. J Reconstr Microsurg 2008;24:159–67.
• 10. Piskin A, Kaplan S, Aktas A, et al. Platelet gel does not improve peripheral nerve regeneration: An electrophysiological, stereological, and electron microscopic study. Microsurgery 2009; 29:144–53.
• 11. Şenses F, Önder ME, Koçyiğit ID, et al. Effect of Platelet-Rich Fibrin on Peripheral Nerve Regeneration. J Craniofac Surg. 2016;27: 1759-64.
• 12. Lichtenfels M, Colomé L, Sebben AD, et al. Effect of Platelet Rich Plasma and Platelet Rich Fibrin on sciatic nerve regeneration in a rat model. Microsurgery 2013;33: 383-90.
• 13. Giannessi E, Coli A, Stornelli MR, et al. An autologously generated platelet-rich plasma suturable membrane may enhance peripheral nerve regeneration after neurorraphy in an acute injury model of sciatic nerve neurotmesis. J Reconstr Microsurg. 2014;30:617-26. 14. Uysal AC, Mizuno H. Tendon regeneration and repair with adipose derived stem cells. Current stem cell research & therapy 2010; 5:161-7.
• 15. Ehrenfest D, David M. Slow release of growth factors and thrombospondin-1 in Choukroun's platelet-rich fibrin (PRF): a gold standard to achieve for all surgical platelet concentrates technologies. Growth Factors 2009; 27:63-9.
• 16. Apel PJ, Ma J, Callahan M, et al. Effect of locally delivered IGF-1 onnerve regeneration during aging: an experimental study inrats. Muscle Nerve 2010; 41:335-41.
• 17. Koriyama Y, Homma K, Sugitani K, et al. Upregulation of IGF-I in the goldfish retinal ganglion cells during the early stage of opticnerve regeneration. Neurochem Int 2007; 50:749-56.
• 18. Haas SL, Fitzner B, Jaster R, et al. Transforming growth factor-beta inducesnerve growth factor expression in pancreatic stellatecells by activation of the ALK-5 pathway. Growth Factors 2009; 27:289-99.
• 19. Choukroun J, Diss A, Simonpieri A, et al. Platelet-rich fibrin (PRF): A second-generationplatelet concentrate. Part V: Histologic evaluations of PRF effectson bone allograft maturation in sinus lift. Oral Surg Oral Med Oral PatholOral Radiol Oral Endod 2006; 101:299-303.
• 20. Rosenstein JM, Krum JM. New roles for VEGF innervous tissue-beyond blood vessels. Exp Neurol 2004; 187:246-53.
• 21. Dohan D M, Diss A, Odin G, et al. In vitro effectsof Choukroun’s PRF on human gingival fibroblasts,dermalpre keratinocytes, preadipocytes and maxillofacial osteoblasts in primarycultures. Oral Surg Oral Med Oral Pathol Oral Radiol Oral Endod 2009;108:341-52.