Gebelikte Tütün Dumanı Maruziyetinin Anne Sıçan Akciğer Dokusunda Meydana Getirdiği Değişiklikler Üzerine Alfa Lipoik Asitin Etkilerinin İncelenmesi

Year 2018, Volume: 45 Issue: 3, 297 - 307, 05.09.2018

Elif Erdem GÜZEL Nalan KAYA Gonca Ozan KOCAMÜFTÜOĞLU Mehmet Ali KISAÇAM Dürrin Özlem DABAK İbrahim Enver OZAN

https://doi.org/10.5798/dicletip.457249

Abstract

Amaç: Çalışmamızda gebelikte tütün dumanına maruz
kalan anne sıçanların akciğer dokusunda meydana gelen değişiklikler üzerine
alfa lipoik asitin etkilerinin deneysel sıçan modeli üzerinde araştırılması
amaçlandı.

Yöntemler: Çalışmada 28 adet dişi Sprague-Dawley cinsi
sıçanlar kullanıldı. Gebe sıçanlar; Kontrol grubu, Tütün dumanı (TD) grubu,
Tütün dumanı + Alfa lipoik asit (TD+ALA) grubu ve Alfa lipoik asit (ALA) grubu
olmak üzere rastgele dört eşit gruba ayrıldı. TD ve TD+ALA grubundaki sıçanlar
çiftleşmeden önce sekiz hafta ve gebelik süresince günde iki saat tütün
dumanına maruz bırakıldı.

TD+ALA ve ALA grubundaki sıçanlara ise çiftleşmeden
önce sekiz hafta ve gebelik süresince gün aşırı oral gavaj yolu ile 20 mg/kg
dozunda alfa lipoik asit verildi. Deneyin sonunda sıçanlar dekapite edilerek
akciğer dokuları çıkarıldı ve histolojik, biyokimyasal ve immünohistokimyasal
metotlar uygulandı.

Bulgular: TD grubuna ait akciğer kesitlerinde
inflamatuar hücre artışı, konjesyon, ödem, hemoraji gibi histopatolojik
bulgular gözlendi. ALA uygulamasıyla bu histopatalojik bulgularda istatistiksel
olarak anlamlı oranda düzelmeler izlendi. TD grubunda VEGF immünreaktivitesinin
kontrol grubuna göre anlamlı artış gösterdiği, TD+ALA grubunda ise TD grubuna
göre VEGF immünreaktivitesinin anlamlı derecede azaldığı belirlendi. TD
grubunda MDA değerlerinin kontrole göre anlamlı derecede arttığı, TD+ALA
grubunda ise TD grubuna göre anlamlı derecede azaldığı gözlendi.

Sonuç: Tütün dumanının gebe sıçan akciğerinde yol
açtığı oksidatif hasarın, alfa lipoik asit tedavisinin antioksidan etkileri ile
kısmen engellendiği belirlendi. 

Keywords

Gebelik, tütün dumanı, alfa lipoik asit, akciğer

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