Türk Populasyonunda Şizofreni Hastalığı İçin Bir Risk Faktörü Olarak Adenozin Deaminaz Geni G22a Polimorfizmi

Year 2016, Volume: 43 Issue: 2, 218 - 223, 01.06.2016

Aydın Rüstemoğlu Birgül Elbozan Cumurcu Ayşe Feyda Nursal Mahmut Balkan Melike Uzun Serbülent Yiğit

Abstract

Amaç: Bu çalışmanın amacı, adenozin deaminaz (ADA)
genindeki G22A polimorfizminin Türk popülasyonunda şizofreni
ile ilişkisinin araştırılmasıdır.
Yöntemler: Bu çalışmada biz 113 şizofreni tanısı almış
hasta ve 121 sağlıklı kontrol bireylerini inceledik. ADA
geni G22A polimorfizmi allel spesifik-polimeraz zincir reaksiyonu
(AS-PCR) yöntemi kullanılarak belirlendi.
Bulgular: ADA genindeki G22A polimorfizmi için GG,
GA ve AA genotip sıklıkları sırası ile kontrol grubu için
%79.3 (96/121), %20,3 (25/121), %0 (0) ve hasta grubu
için %87,6 (99/113), %10,6 (12/113), %1,8 (2/113) olarak
belirlendi. Genotip dağılım bakımından hasta ve kontrol
grupları arasında anlamlı fark bulundu (p=0,017). Ayrıca
GA genotipinin sıklığı hastalarda kontrole nazaran anlamlı
düzeyde düşük bulundu (p=0,048, OR=0.46, 95%CI 0.22-
0.96). Fakat allel sıklıkları bakımından iki grup arasında
anlamlı fark bulunamadı. Cinsiyet bakımından karşılaştırma
yaptığımız zaman, erkek hastalarda GG genotipinin
anlamlı düzeyde yüksek, GA genotipinin ise düşük olduğu
gözlendi (sırası ile p=0,036 ve p=0,005). Kadın hastalarda
herhangi bir anlamlı fark gözlenmedi.
Sonuç: Bizim sonuçlar ADA genindeki G22A polimorfizminin
Türk popülasyonunda şizofreni hastalığı ile ilişkili
olabileceğini gösterdi. ADA genindeki GA genotipinin
özellikle erkeklerde şizofreniye yatkınlığı düşürdüğü,
buna karşın GG genotipinin yatkınlığı arttırdığı muhtemeldir.
Farklı hasta ve etnik gruplar ile gelecekte yapılacak
çalışmalar, elde ettiğimiz sonuçların kesinleşmesine
yardımcı olur

Keywords

Şizofreni, ADA geni, G22A polimorfizmi

Adenosine Deaminase Gene G22a Polymorphism as a Risk Factor for Schizophrenia in Turkish Population

Abstract

Objective: The aim of this study was to investigate whether the G22A polymorphism of adenosine deami­nase (ADA) gene is associated with schizophrenia in Turkish population. Methods: In this study, we evaluated 113 patients with schizophrenia and 121 individuals without the disease. The ADA G22A polymorphism was examined using allele specific-polymerase chain reaction (PCR). Results: The ADA G22A genotype frequencies of GG, GA, and AA were 79.3% (96/121), 20.7% (25/121) and 0% (0) in the control group, while 87.6 % (99/113), 10.6% (12/113), 1.8% (2/113) in the patient group, respectively. There was a statistically significant difference in genotype distribution between patients and controls (p=0.017). Also the frequency of GA genotype was found significantly low­er in patients compared with healthy controls (OR = 0.46, 95% Cl 0.22-0.96, p =0.048). However, there was not any noticeable difference in allele distribution between the groups (p>0.05). In addition, the frequency of GG geno­type in the male patient group significantly higher, and GA genotype significantly lower compared to the male control group, were found (p=0.036, p=0.005, respectively). No association was found for the female group. Conclusion: Our results show that, the G22A polymor­phism of ADA gene may be associated with schizophrenia in Turkish population. The ADA GA genotype is likely to reduce, whereas GG genotype increased genetic sus­ceptibility to schizophrenia, especially in males. Further studies should be repeated with different study subjects and/or other ethnic subjects to generalize the conclusion of this study.

Keywords

Schizophrenia, ADA gene, G22A polymorphism

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