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In-vitro activity of fosfomycin trometamol and some other antibiotics against Escherichia coli strains isolated from urinary tract infections

Year 2011, Volume: 38 Issue: 3, 298 - 300, 01.09.2011
https://doi.org/10.5798/diclemedj.0921.2011.03.0035

Abstract

Objectives: In recent years, in our country and the world, susceptibility of uropathogenic E.coli strains to commonly used antibiotics in the treatment of urinary tract infections has decreased. That decreased susceptibility has been caused to treatment failure, need to change the empirical treatment, increase in prescription costs, prolongation of hospital stay, increased social costs, increased morbidity and mortality. In this study, determination of susceptibility to different variety of antibiotics and fosfomycin in urinary tract infection isolate E.coli were aimed. Materials and methods: The study included E.coli posi­tive urine samples which had been sent to State Hospi­tal Central Laboratory in Kızıltepe between August 2010 and December 2010. These isolates were obtained from patients who have microscopically 10/mm3 leukocyte in urine. In this study, we tested susceptibility of iso­lated E.coli to FOT (Fosfomycin), CIP(ciprofloxacin), IP (İmipenem), TZP (Piperasilin-Tazobactam), CAZ (Ceftazi­dim). According to CLSI criteria, susceptibility testing was performed by Kirby-Bauer disc diffusion method. Results: E.coli strains isolated from urine samples exam­ined between August 2010 and December 2010, all were susceptible to fosfomisine. The resistance rates of E.coli strains to Ceftazidim, Ciprofloxacin, İmipenem, Piperacil­lin-Tazobactam were found respectively 33.3%, 49.1%, 36.8%, 45.6% (Table 1). Conclusions: Because of the high susceptibility rates of fosfomycin at community-acquired urinary tract infection, it can be preferred to the non-complicated urinary tract infections as an antimicrobial agent.

References

  • Hooton TM. Pathogenesis of urinary tract infections: an update. J Antimicrob Chemother 2000; 46(Suppl 1):1-7.
  • Kaygusuz S, Apan T Z, Kılıç D. Toplum kökenli üriner sistem infeksiyonu etkeni Gram negatif bakterilerde çeşitli antibiyotik- lere direnç. Ankem Derg 2001; 15(4):753-9.
  • Goettsch W, Van Plet W, Nagelkerke N, et al. Increasing resis- tance to flouroquinolones in Escherichia coli from urinary tract infections in the Netherlands. J Antimicrob Chemother 2000; 46(2):223-8.
  • Hunter PA, and Reeves DS. The current status of surveillance of resistance to antimicrobial agents: report on a meeting. J Antimi- crob Chemother 2002; 49(1):17-23.
  • Greenwood Davıd. Inhibitors of bacterial cell wall synthesis. Anti- microbial Chemotherapy, p.16, Oxford Universtiy Pres, 1994.
  • National Committee for Clinical Laboratory Standards. Perfor- mance Standards for Antimicrobial Disk Susceptibility Tests. Approved Standard. NCCLS Document M2-S42-45. Wayne, 2002.
  • Ertuğrul MB, Atla-Güleç L, Akal D, et al. Üropatojen E.coli Suşla- rının tedavide sık kullanılan antibiyotiklere duyarlılıkları Klimik Derg 2009;17(2):132-6.
  • Kahlmeter G. An international survey of the antimicrobial sus- ceptibility of pathogens from uncomplicated urinary tract in- fections: the ECO.SENS Project. J Antimicrob Chemother 2003;51(1):69-76.
  • Fadda G, Nicoletti G, Schito GC, Tempera G. Antimicrobial susceptibility patterns of contemporary pathogens from un- complicated urinary tract infections isolated in a multicenter Italian survey: possible impact on guidelines. J Chemoter 2005 ;17(3):251-7.
  • Alós JI, Serrano MG, Gómez-Garcés JL, Perianes J. Antibiotic resistance of Escherichia coli from community-acquired urinary tract infections in relation to demographic and clinical data. Clin Microbiol Infect 2005 ;11(3):199-203.
  • Kahlmeter G. Prevalence and antimicrobial susceptibility of pathogens in uncomplicated cystitis in Europe. The ECO.SENS study. Int J Antimicrob Agents 2003 ;22 (Suppl2) :49-52.
  • Ungheri D, Albini E, Belluco G. In-vitro susceptibility of qui- nolone-resistant clinical isolates of Escherichia coli to fosfomi- sin trometamol. J Chemother 2002 ;14(3):237-40.
  • Arslan H, Azap OK, Ergonul O, Timurkaynak F. Urinary Tract Infection Study Group: Risk factors for ciprofloxasin resistance among Escherichia coli strains isolated from community-ac- quired urinary tract infections in Turkey. J Antimicrob Chemoter 2005; 56(5): 914-8.
  • Köken G, Aşık G, Çiftçi İH, Çetinkaya Z, Aktepe OC, Yılmazer M. Toplum kökenli üriner sistem infeksiyonu etkeni Escherichia coli suşlarında fosfomisin trometamol etkinliği. ANKEM Derg 2008;22(1):23-7.
  • Uyanık H, Hancı H, Yazgı H, Üriner sistem infeksiyonlarından soyutlanan toplum kökenli Escherichia coli suşlarına fosfomisin trometamolün ve bazı antibiyotiklerin in-vitro etkinliği, AN- KEM Derg 2009;23(4):172-6.
  • Rifaioğlu MM, Yıldırım A, Başok EK, et al. Son dört yıl içe- risinde idrar kültürlerinden izole edilen bakterilere karşı ge- lişen antibiyotik direncindeki değişim. Türk Üroloji Dergisi 2009;35(3):201-9.

In-vitro activity of fosfomycin trometamol and some other antibiotics against Escherichia coli strains isolated from urinary tract infections

Year 2011, Volume: 38 Issue: 3, 298 - 300, 01.09.2011
https://doi.org/10.5798/diclemedj.0921.2011.03.0035

Abstract

Amaç: Son yıllarda ülkemizde ve dünyada üropatojen E.coli suşlarında üriner sistem infeksiyonu tedavisinde sık kullanılan antibiyotiklere karşı duyarlılıkta azalma gö­rülmektedir. Direnç nedeniyle tedavi yetersizliği ve ampi­rik tedavinin değiştirilmesi ihtiyacı, reçete maliyetlerinde artış, hastanede kalma süresinde uzama, sosyal maliyet, morbidite ve mortalitede artış ortaya çıkmaktadır. Bu ça­lışmada, üriner sistem infeksiyon etkeni olan E.coli\'nin bazı antibiyotiklere ve fosfomisine olan duyarlılığının araştırılması amaçlanmıştır. Gereç ve yöntem: Kızıltepe Devlet Hastanesi Merkez laboratuarına Ağustos 2010-Aralık 2010 tarihleri arasın­da polikliniklerden gelen idrar örneklerinden izole edilen E.coli izolatları çalışmaya alınmıştır. Bu izolatlar idrar mik­roskobisinde 10/mm3 lökositi olup üriner sistem infeksi­yonu düşündüğümüz hastaların idrar kültürlerinden elde edilmiştir. Bu çalışmada üropatojen E.coli infeksiyonların­da FOT (fosfomisin), CIP (Siprofloksasin), IP (İmipenem), TZP (Piperasilin-Tazobactam), CAZ (Ceftazidim)\'in duyar­lılıklarına bakılmıştır. Antibiotik duyarlılıkları CLSI (Clinical Laboratory Standards Institute) önerileri doğrultusunda Kirby-Bauer disk difüzyon yöntemi ile değerlendirilmiştir. Kontrol suşu olarak E.coli ATCC 25922 kullanılmıştır. Bulgular: Ağustos 2010-Aralık 2010 tarihleri arasında in­celenen idrar örneklerinden izole edilen E.coli suşlarının hepsi fosfomisine duyarlı bulunmuştur. Siprofloksasine, İmipenem, Piperasilin-Tazobaktam, Seftazidime karşı di­renç oranları sırasıyla; %33,3, %49,1, %36,8, %45,6 ola­rak belirlenmiştir. Sonuç: Toplum kökenli üriner sistem infeksiyonlarında fosfomisin; yüksek düzey duyarlılık oranları nedeni ile non-komplike üriner sistem enfeksiyonlarında tercih edi­lebilir bir antimikrobiyal ajandır.

References

  • Hooton TM. Pathogenesis of urinary tract infections: an update. J Antimicrob Chemother 2000; 46(Suppl 1):1-7.
  • Kaygusuz S, Apan T Z, Kılıç D. Toplum kökenli üriner sistem infeksiyonu etkeni Gram negatif bakterilerde çeşitli antibiyotik- lere direnç. Ankem Derg 2001; 15(4):753-9.
  • Goettsch W, Van Plet W, Nagelkerke N, et al. Increasing resis- tance to flouroquinolones in Escherichia coli from urinary tract infections in the Netherlands. J Antimicrob Chemother 2000; 46(2):223-8.
  • Hunter PA, and Reeves DS. The current status of surveillance of resistance to antimicrobial agents: report on a meeting. J Antimi- crob Chemother 2002; 49(1):17-23.
  • Greenwood Davıd. Inhibitors of bacterial cell wall synthesis. Anti- microbial Chemotherapy, p.16, Oxford Universtiy Pres, 1994.
  • National Committee for Clinical Laboratory Standards. Perfor- mance Standards for Antimicrobial Disk Susceptibility Tests. Approved Standard. NCCLS Document M2-S42-45. Wayne, 2002.
  • Ertuğrul MB, Atla-Güleç L, Akal D, et al. Üropatojen E.coli Suşla- rının tedavide sık kullanılan antibiyotiklere duyarlılıkları Klimik Derg 2009;17(2):132-6.
  • Kahlmeter G. An international survey of the antimicrobial sus- ceptibility of pathogens from uncomplicated urinary tract in- fections: the ECO.SENS Project. J Antimicrob Chemother 2003;51(1):69-76.
  • Fadda G, Nicoletti G, Schito GC, Tempera G. Antimicrobial susceptibility patterns of contemporary pathogens from un- complicated urinary tract infections isolated in a multicenter Italian survey: possible impact on guidelines. J Chemoter 2005 ;17(3):251-7.
  • Alós JI, Serrano MG, Gómez-Garcés JL, Perianes J. Antibiotic resistance of Escherichia coli from community-acquired urinary tract infections in relation to demographic and clinical data. Clin Microbiol Infect 2005 ;11(3):199-203.
  • Kahlmeter G. Prevalence and antimicrobial susceptibility of pathogens in uncomplicated cystitis in Europe. The ECO.SENS study. Int J Antimicrob Agents 2003 ;22 (Suppl2) :49-52.
  • Ungheri D, Albini E, Belluco G. In-vitro susceptibility of qui- nolone-resistant clinical isolates of Escherichia coli to fosfomi- sin trometamol. J Chemother 2002 ;14(3):237-40.
  • Arslan H, Azap OK, Ergonul O, Timurkaynak F. Urinary Tract Infection Study Group: Risk factors for ciprofloxasin resistance among Escherichia coli strains isolated from community-ac- quired urinary tract infections in Turkey. J Antimicrob Chemoter 2005; 56(5): 914-8.
  • Köken G, Aşık G, Çiftçi İH, Çetinkaya Z, Aktepe OC, Yılmazer M. Toplum kökenli üriner sistem infeksiyonu etkeni Escherichia coli suşlarında fosfomisin trometamol etkinliği. ANKEM Derg 2008;22(1):23-7.
  • Uyanık H, Hancı H, Yazgı H, Üriner sistem infeksiyonlarından soyutlanan toplum kökenli Escherichia coli suşlarına fosfomisin trometamolün ve bazı antibiyotiklerin in-vitro etkinliği, AN- KEM Derg 2009;23(4):172-6.
  • Rifaioğlu MM, Yıldırım A, Başok EK, et al. Son dört yıl içe- risinde idrar kültürlerinden izole edilen bakterilere karşı ge- lişen antibiyotik direncindeki değişim. Türk Üroloji Dergisi 2009;35(3):201-9.
There are 16 citations in total.

Details

Primary Language Turkish
Journal Section Research Articles
Authors

Özcan Deveci This is me

Erkan Yula This is me

Türkan Toka Özer This is me

Alicem Tekin This is me

Publication Date September 1, 2011
Submission Date March 2, 2015
Published in Issue Year 2011 Volume: 38 Issue: 3

Cite

APA Deveci, Ö., Yula, E., Özer, T. T., Tekin, A. (2011). In-vitro activity of fosfomycin trometamol and some other antibiotics against Escherichia coli strains isolated from urinary tract infections. Dicle Tıp Dergisi, 38(3), 298-300. https://doi.org/10.5798/diclemedj.0921.2011.03.0035
AMA Deveci Ö, Yula E, Özer TT, Tekin A. In-vitro activity of fosfomycin trometamol and some other antibiotics against Escherichia coli strains isolated from urinary tract infections. diclemedj. September 2011;38(3):298-300. doi:10.5798/diclemedj.0921.2011.03.0035
Chicago Deveci, Özcan, Erkan Yula, Türkan Toka Özer, and Alicem Tekin. “In-Vitro Activity of Fosfomycin Trometamol and Some Other Antibiotics Against Escherichia Coli Strains Isolated from Urinary Tract Infections”. Dicle Tıp Dergisi 38, no. 3 (September 2011): 298-300. https://doi.org/10.5798/diclemedj.0921.2011.03.0035.
EndNote Deveci Ö, Yula E, Özer TT, Tekin A (September 1, 2011) In-vitro activity of fosfomycin trometamol and some other antibiotics against Escherichia coli strains isolated from urinary tract infections. Dicle Tıp Dergisi 38 3 298–300.
IEEE Ö. Deveci, E. Yula, T. T. Özer, and A. Tekin, “In-vitro activity of fosfomycin trometamol and some other antibiotics against Escherichia coli strains isolated from urinary tract infections”, diclemedj, vol. 38, no. 3, pp. 298–300, 2011, doi: 10.5798/diclemedj.0921.2011.03.0035.
ISNAD Deveci, Özcan et al. “In-Vitro Activity of Fosfomycin Trometamol and Some Other Antibiotics Against Escherichia Coli Strains Isolated from Urinary Tract Infections”. Dicle Tıp Dergisi 38/3 (September 2011), 298-300. https://doi.org/10.5798/diclemedj.0921.2011.03.0035.
JAMA Deveci Ö, Yula E, Özer TT, Tekin A. In-vitro activity of fosfomycin trometamol and some other antibiotics against Escherichia coli strains isolated from urinary tract infections. diclemedj. 2011;38:298–300.
MLA Deveci, Özcan et al. “In-Vitro Activity of Fosfomycin Trometamol and Some Other Antibiotics Against Escherichia Coli Strains Isolated from Urinary Tract Infections”. Dicle Tıp Dergisi, vol. 38, no. 3, 2011, pp. 298-00, doi:10.5798/diclemedj.0921.2011.03.0035.
Vancouver Deveci Ö, Yula E, Özer TT, Tekin A. In-vitro activity of fosfomycin trometamol and some other antibiotics against Escherichia coli strains isolated from urinary tract infections. diclemedj. 2011;38(3):298-300.