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Fusidic acid resistance among staphylococci strains isolated from clinical specimens in a general hospital

Year 2012, Volume: 39 Issue: 1, 1 - 5, 01.03.2012
https://doi.org/10.5798/diclemedj.0921.2012.01.0084

Abstract

Objectives: The aim of this study was to investigate in vitro susceptibility of fusidic acid to clinic isolates of staphylo-cocci. Materials and methods: The forty-one coagulase negative staphylococci (CNS) and 18 Staphylococcus aureus strains isolated from various clinical specimens were included in this study. Staphylococci isolates were identified by conventional methods such as colony morphology onto medium, gram staining, catalase and coagulase tests. Ac-cording to “Clinical and Laboratory Standards Institute (CLSI)” criteria, antimicrobial susceptibility testing of isolates was performed by Kirby-Bauer\'s disk diffusion method. Results: The seventy-two percent of the isolated S.aureus were defined as methicillin sensitive-S.aureus (MSSA), 28% of the isolated S.aureus were defined as methicillin resistant-S.aureus (MRSA). The difference among fusidic acid susceptibility rates of MSSA and MRSA strains was not statistically significant (p=0.305). The twenty-nine per-cent of the isolated CNS were defined as methicillin sensitive-CNS (MS-CNS), 71% of the isolated CNS were defined as methicillin resistant-CNS (MR-CNS). There was no statistically significant difference between MS-CNS and MR-CNS strains for fusidic acid susceptibility rates (p=0.490). But the difference among fusidic acid susceptibility rates of CNS and S.aureus strains was statistically significant (p

References

  • Castanheira M, Watters AA, Mendes RE, Farrell DJ, Jones RN. Occurrence and molecular characterization of fusidic acid resistance mechanisms among Staphylococcus spp. from European countries (2008). J Antimicrob Chemother 2010;65(7):1353-8.
  • Castanheira M, Watters AA, Bell JM, Turnidge JD, Jones RN. Fusidic acid resistance rates and prevalence of resis- tance mechanisms among Staphylococcus spp. isolated in North America and Australia, 2007-2008. Antimicrob Agents Chemother 2010;54(9):3614-7.
  • Bodley JW, Zieve FJ, Lin L, Zieve ST. Formation of the ri- bosome-G factor-GDP complex in the presence of fusidic acid. Biochem Biophys Res Commun 1969;37(3):437-43.
  • Gao YG, Selmer M, Dunham CM, Weixlbaumer A, Kelley AC, Ramakrishnan V. The structure of the ribosome with elongation factor G trapped in the posttranslocational state. Science 2009;326(5953):694-9.
  • Chen CM, Huang M, Chen HF, et al. Fusidic acid resistance among clinical isolates of methicillin-resistant Staphylo- coccus aureus in a Taiwanese hospital. BMC Microbiol 2011;11(1):98-102.
  • Clinical and Laboratory Standards Institute. Performance standards for antimicrobial susceptibility testing. Twenty- first informational supplement. CLSI, M100-S21, 2011. Wayne, Pennsylvania, USA.
  • The European Committee on Antimicrobial Susceptibility Testing (EUCAST): Breakpoint tables for interpretations of MICs and zone diameters. EUCAST Clinical Breakpoint Table v. 1.1 2010-04-27. (http://www.eucast.org/fileadmin/ src/media/PDFs)
  • Farrell DJ, Castanheira M, Chopra I. Characterization of global patterns and the genetics of fusidic acid resistance. Clin Infect Dis 2011;52(Suppl 7):487-92.
  • McLaws FB, Larsen AR, Skov RL, Chopra I, O’Neill AJ. Distribution of fusidic acid resistance determinants in methicillin-resistant Staphylococcus aureus. Antimicrob Agents Chemother 2011;55(3):1173-6.
  • Chen HJ, Hung WC, Tseng SP, Tsai JC, Hsueh PR, Teng LJ. Fusidic Acid Resistance Determinants in Staphylococ- cus aureus Clinical Isolates. Antimicrob Agents Chemother 2010;54(12):4985-91.
  • Fernandes P, Pereira D. Efforts to support the develop- ment of fusidic acid in the United States. Clin Infect Dis 2011;52(Suppl 7):542-6.
  • Keşli R, Cander S, Çelebi S. Fusidic Acid Resistance of Methicillin Resistant and Sensitive Staphylococcus Strains Isolated From Clinical Specimens. Kocatepe Tıp Dergisi 2004;5(2):33-6.
  • Kuzucu Ç, Dalgalar M, Durmaz B. Metisiline dirençli Stap- hylococcus aureus ve koagülaz negatif stafilokoklarda fusi- dik asit duyarlılığı. ANKEM Derg 2003;17(1):7-9.
  • Uluğ M, Ayaz C, Celen MK. Fusidic acid’s place in the treatment of chronic staphylococcal osteomyelitis. Anatol J Clin Invest 2009;3(3):222-6.
  • Erdemoğlu A, Özsoy MF, Emekdaş G, Öncül O, Pahsa A. The resistance of Staphylococci isolated from urine to fu- sidic acid and other antimicrobials. Türk Mikrobiol Cem Derg 2000;30(1):6-12.
  • Altun B, Kocagöz S, Hasçelik G, Uzun O, Akova M, Ünal S. Susceptibilities to fusidic acid and frequently used anti- biotics of Staphylococcus strains isolated in various hospi- tals. Türk Mikrobiyol Cem Derg 2003;33(1):8-11.
  • Şengöz G, Yıldırım F, Kart-Yaşar K, Şengöz A, Nazlıcan O. Resistance of Staphylococcus strains against fusidic acid and other antibiotics. Ankem Derg 2004;18(1):105-8.
  • Çelen MK, Ayaz C, Özmen E, Geyik MF, Hoşoğlu S. Re- sistance to fucidic acid in clinical Staphylococcus aureus isolates. Klimik Derg 2005;18(1);114-6.
  • Nergiz S, Özekinci T, Gülhan B, Meşe S, Atmaca S. Re- sistance to fusidic acid in methicillin-resistant coagulase negative Staphylococci isolated from various clinical speci- mens. Ankem Derg 2007;21(2):228-31.
  • Ekşi F, Gayyurhan ED, Bayram A. Antimicrobial suscepti- bility of Staphylococcus aureus strains isolated in Gazian- tep University Hospital. Ankem Derg 2008;22(2):203-8.
  • Mert Dinç B, Karabiber N, Aykut Arca E. Macrolide-Lin- cosamide-Streptogramin B (MLSB) resistance and fusidic acid susceptibility of methicillin resistant Staphylococcus aureus (MRSA) strains isolated from clinical samples. Türk Hijyen ve Deneysel Biyoloji Derg 2009;66(1):89-94.
  • Yaman G, Çıkman A, Berktaş M, Parlak M, Güdücüoğlu H, Karahocagil MK. The MLSB, fusidic acid and various antibiotic resistance rates of nosocomial Staphylococcus aureus isolates. Ankem Derg 2010;24(1):130-5.
  • Deveci Ö, Kılıç D, Kaygusuz S, et al. Evaluation of resis- tance to fusidic acid in Staphylococci isolates. J Microbiol Infect Dis 2011;1(1):22-5.

Fusidic acid resistance among staphylococci strains isolated from clinical specimens in a general hospital

Year 2012, Volume: 39 Issue: 1, 1 - 5, 01.03.2012
https://doi.org/10.5798/diclemedj.0921.2012.01.0084

Abstract

Amaç: Bu çalışmanın amacı, klinik örneklerden izole edilen stafilokok suşlarında fusidik asidin in vitro etkinliğinin araştırılmasıdır. Gereç ve yöntem: Çalışmaya çeşitli klinik örneklerden izole edilen 41 koagülaz negatif stafilokok (KNS) izolatı ile 18 Staphylococcus aureus suşu dahil edildi. Stafilokok izolatları besiyeri yüzeyindeki koloni morfolojisi, gram boyama, katalaz ve koagülaz testleri gibi konvansiyonel yöntemlerle identifiye edildi. İzolatların antimikrobiyal duyarlılıkları “Clinical and Laboratory Standards Institute (CLSI)” önerileri doğrultusunda Kirby-Bauer disk difüzyon yöntemi kulla-nılarak çalışıldı. Bulgular: İzole edilen S.aureus suşlarının % 72\'si metisiline duyarlı (MSSA), % 28\'i metisiline dirençli (MRSA) olarak tanımlandı. MSSA ve MRSA suşlarının fusidik asit duyarlılık oranları arasındaki fark istatistiksel olarak anlamlı bu-lunmadı (p=0.305). İzole edilen KNS\'lerin % 29\'u metisiline duyarlı (MS-KNS), % 71\'i metisiline dirençli (MR-KNS) olarak tanımlandı. MR-KNS ve MS-KNS suşlarının fusidik asit duyarlılık oranları arasında istatistiksel olarak anlamlı fark yoktu (p=0.490). Ancak, KNS ve S.aureus suşlarının fusidik asit duyarlılık oranları arasındaki fark istatistiksel ola-rak anlamlıydı (p

References

  • Castanheira M, Watters AA, Mendes RE, Farrell DJ, Jones RN. Occurrence and molecular characterization of fusidic acid resistance mechanisms among Staphylococcus spp. from European countries (2008). J Antimicrob Chemother 2010;65(7):1353-8.
  • Castanheira M, Watters AA, Bell JM, Turnidge JD, Jones RN. Fusidic acid resistance rates and prevalence of resis- tance mechanisms among Staphylococcus spp. isolated in North America and Australia, 2007-2008. Antimicrob Agents Chemother 2010;54(9):3614-7.
  • Bodley JW, Zieve FJ, Lin L, Zieve ST. Formation of the ri- bosome-G factor-GDP complex in the presence of fusidic acid. Biochem Biophys Res Commun 1969;37(3):437-43.
  • Gao YG, Selmer M, Dunham CM, Weixlbaumer A, Kelley AC, Ramakrishnan V. The structure of the ribosome with elongation factor G trapped in the posttranslocational state. Science 2009;326(5953):694-9.
  • Chen CM, Huang M, Chen HF, et al. Fusidic acid resistance among clinical isolates of methicillin-resistant Staphylo- coccus aureus in a Taiwanese hospital. BMC Microbiol 2011;11(1):98-102.
  • Clinical and Laboratory Standards Institute. Performance standards for antimicrobial susceptibility testing. Twenty- first informational supplement. CLSI, M100-S21, 2011. Wayne, Pennsylvania, USA.
  • The European Committee on Antimicrobial Susceptibility Testing (EUCAST): Breakpoint tables for interpretations of MICs and zone diameters. EUCAST Clinical Breakpoint Table v. 1.1 2010-04-27. (http://www.eucast.org/fileadmin/ src/media/PDFs)
  • Farrell DJ, Castanheira M, Chopra I. Characterization of global patterns and the genetics of fusidic acid resistance. Clin Infect Dis 2011;52(Suppl 7):487-92.
  • McLaws FB, Larsen AR, Skov RL, Chopra I, O’Neill AJ. Distribution of fusidic acid resistance determinants in methicillin-resistant Staphylococcus aureus. Antimicrob Agents Chemother 2011;55(3):1173-6.
  • Chen HJ, Hung WC, Tseng SP, Tsai JC, Hsueh PR, Teng LJ. Fusidic Acid Resistance Determinants in Staphylococ- cus aureus Clinical Isolates. Antimicrob Agents Chemother 2010;54(12):4985-91.
  • Fernandes P, Pereira D. Efforts to support the develop- ment of fusidic acid in the United States. Clin Infect Dis 2011;52(Suppl 7):542-6.
  • Keşli R, Cander S, Çelebi S. Fusidic Acid Resistance of Methicillin Resistant and Sensitive Staphylococcus Strains Isolated From Clinical Specimens. Kocatepe Tıp Dergisi 2004;5(2):33-6.
  • Kuzucu Ç, Dalgalar M, Durmaz B. Metisiline dirençli Stap- hylococcus aureus ve koagülaz negatif stafilokoklarda fusi- dik asit duyarlılığı. ANKEM Derg 2003;17(1):7-9.
  • Uluğ M, Ayaz C, Celen MK. Fusidic acid’s place in the treatment of chronic staphylococcal osteomyelitis. Anatol J Clin Invest 2009;3(3):222-6.
  • Erdemoğlu A, Özsoy MF, Emekdaş G, Öncül O, Pahsa A. The resistance of Staphylococci isolated from urine to fu- sidic acid and other antimicrobials. Türk Mikrobiol Cem Derg 2000;30(1):6-12.
  • Altun B, Kocagöz S, Hasçelik G, Uzun O, Akova M, Ünal S. Susceptibilities to fusidic acid and frequently used anti- biotics of Staphylococcus strains isolated in various hospi- tals. Türk Mikrobiyol Cem Derg 2003;33(1):8-11.
  • Şengöz G, Yıldırım F, Kart-Yaşar K, Şengöz A, Nazlıcan O. Resistance of Staphylococcus strains against fusidic acid and other antibiotics. Ankem Derg 2004;18(1):105-8.
  • Çelen MK, Ayaz C, Özmen E, Geyik MF, Hoşoğlu S. Re- sistance to fucidic acid in clinical Staphylococcus aureus isolates. Klimik Derg 2005;18(1);114-6.
  • Nergiz S, Özekinci T, Gülhan B, Meşe S, Atmaca S. Re- sistance to fusidic acid in methicillin-resistant coagulase negative Staphylococci isolated from various clinical speci- mens. Ankem Derg 2007;21(2):228-31.
  • Ekşi F, Gayyurhan ED, Bayram A. Antimicrobial suscepti- bility of Staphylococcus aureus strains isolated in Gazian- tep University Hospital. Ankem Derg 2008;22(2):203-8.
  • Mert Dinç B, Karabiber N, Aykut Arca E. Macrolide-Lin- cosamide-Streptogramin B (MLSB) resistance and fusidic acid susceptibility of methicillin resistant Staphylococcus aureus (MRSA) strains isolated from clinical samples. Türk Hijyen ve Deneysel Biyoloji Derg 2009;66(1):89-94.
  • Yaman G, Çıkman A, Berktaş M, Parlak M, Güdücüoğlu H, Karahocagil MK. The MLSB, fusidic acid and various antibiotic resistance rates of nosocomial Staphylococcus aureus isolates. Ankem Derg 2010;24(1):130-5.
  • Deveci Ö, Kılıç D, Kaygusuz S, et al. Evaluation of resis- tance to fusidic acid in Staphylococci isolates. J Microbiol Infect Dis 2011;1(1):22-5.
There are 23 citations in total.

Details

Primary Language Turkish
Journal Section Research Articles
Authors

Türkan Toka Özer This is me

Erkan Yula This is me

Alicem Tekin This is me

Özcan Deveci This is me

Publication Date March 1, 2012
Submission Date March 2, 2015
Published in Issue Year 2012 Volume: 39 Issue: 1

Cite

APA Özer, T. T., Yula, E., Tekin, A., Deveci, Ö. (2012). Fusidic acid resistance among staphylococci strains isolated from clinical specimens in a general hospital. Dicle Tıp Dergisi, 39(1), 1-5. https://doi.org/10.5798/diclemedj.0921.2012.01.0084
AMA Özer TT, Yula E, Tekin A, Deveci Ö. Fusidic acid resistance among staphylococci strains isolated from clinical specimens in a general hospital. diclemedj. March 2012;39(1):1-5. doi:10.5798/diclemedj.0921.2012.01.0084
Chicago Özer, Türkan Toka, Erkan Yula, Alicem Tekin, and Özcan Deveci. “Fusidic Acid Resistance Among Staphylococci Strains Isolated from Clinical Specimens in a General Hospital”. Dicle Tıp Dergisi 39, no. 1 (March 2012): 1-5. https://doi.org/10.5798/diclemedj.0921.2012.01.0084.
EndNote Özer TT, Yula E, Tekin A, Deveci Ö (March 1, 2012) Fusidic acid resistance among staphylococci strains isolated from clinical specimens in a general hospital. Dicle Tıp Dergisi 39 1 1–5.
IEEE T. T. Özer, E. Yula, A. Tekin, and Ö. Deveci, “Fusidic acid resistance among staphylococci strains isolated from clinical specimens in a general hospital”, diclemedj, vol. 39, no. 1, pp. 1–5, 2012, doi: 10.5798/diclemedj.0921.2012.01.0084.
ISNAD Özer, Türkan Toka et al. “Fusidic Acid Resistance Among Staphylococci Strains Isolated from Clinical Specimens in a General Hospital”. Dicle Tıp Dergisi 39/1 (March 2012), 1-5. https://doi.org/10.5798/diclemedj.0921.2012.01.0084.
JAMA Özer TT, Yula E, Tekin A, Deveci Ö. Fusidic acid resistance among staphylococci strains isolated from clinical specimens in a general hospital. diclemedj. 2012;39:1–5.
MLA Özer, Türkan Toka et al. “Fusidic Acid Resistance Among Staphylococci Strains Isolated from Clinical Specimens in a General Hospital”. Dicle Tıp Dergisi, vol. 39, no. 1, 2012, pp. 1-5, doi:10.5798/diclemedj.0921.2012.01.0084.
Vancouver Özer TT, Yula E, Tekin A, Deveci Ö. Fusidic acid resistance among staphylococci strains isolated from clinical specimens in a general hospital. diclemedj. 2012;39(1):1-5.