Effects of Green Light Supplementation with Red and Blue Combinations of LED Light Spectrums On The Growth of Chlamydomonas Reinhardtii (Chlorophyta)

Abstract

Light management strategy regarding optimum spectral composition is a critical factor in microalgae cultivation to improve biomass and biosynthesis of valuable bioactive compounds. Recent advance in LED light technology provides unparallel opportunity to test effects of specific wavelength on physiological response of algae. In this study, we investigated effects of white, monochromatic and combination of red (628 nm) and blue (462 nm) light in the ratio of 1: 1; 2: 1 and 1: 2 at the total light intensity of 40 μmol photons m-2 s-1 on the growth of Chlamydomonas reinhardtii. Moreover, effects of green light (518 nm) supplementation on growth of algae, green light (518 nm) was added gradually into the combination of red:blue (1:2) at the light intensity of 3, 6, 9, 12 and 15 μmol photons m-2 s-1 as an expense of red and blue light intensity at the ratio of 1:2. Results reveal that growth rate of C. reinhadhtii was found in the order of red:blue (1: 2) > red:blue (2: 1) > red:blue (1: 1) > red > white> blue. Green light supplementation applied as 3 μmol photons m-2 s-1 resulted in statistically significant higher optical density and dry weight than R:B (1: 2) used as control group in the experiment. Chlorophyll-a concentrations were found significantly higher in all green light supplementation than control group. Seems that 3 μmol photons m-2 s-1 supplementation of green light together with red:blue combination results in a significant promotion on growth rate, chlorophyll-a and dry weight of C. reinhardtii.

Keywords

• Chlamydomonas reinhardtii
• Green Light Supplementation
• Red Light
• Blue Light
• LED
• Growth Rate

Mavi ve Kırmızı Işık Spektrumları İle Birlikte Yeşil Işık Katkısının Chlamydomonas Reinhardtii (Chlorophyta) Büyümesine Olan Etkileri

Abstract

Mikroalg kültürlerinde uygulanacak optimum ışıklandırma stratejilerinin belirlenmesi, biyoaktif moleküllerin biyosentezi ve biokütle artışı sağlamada önemli bir parametredir. LED ışık teknolojilerinde son yıllarda yaşanan ilerlemeler, belirli ışık spektrumlarının ve bunların farklı karışımlarının mikro alglerin fizyolojik tepkilerine olan etkilerini araştırmak için önemli fırsatlar sağlamaktadır. Bu çalışmada, beyaz ışık, kırmızı (628 nm) ve mavi (462 nm) ışık spektrumları tek başına ve sırasıyla 1:1; 2:1 ve 1:2 oranlarında toplam ışık şiddeti 40 μmol foton m-2 s-1 olacak şekilde Chlamydomonas reinhardtii mikroalg türünün büyümesine olan etkileri araştırılmıştır. Ayrıca, yeşil ışık katkısının mikroalg büyümesine olan etkileri, kırmızı:yeşil (1:2) ışık karışımında 3, 6, 9, 12 ve 15 μmol foton m-2 s-1 şiddetindeki mavi ışık şiddeti azaltılıp yerine yeşil ışık katkısı sağlanarak araştırılmıştır. Elde edilen sonuçlara göre, C. reinhadhtii mikroalg türünün büyüme oranları büyükten küçüğe doğru sırasıyla kırmızı:mavi (1: 2) > kırmızı:mavi (2: 1) > kırmızı:mavi (1: 1) > kırmızı > beyaz> mavi ışık olarak bulunmuştur. Yeşil ışık katkısında ise, 3 μmol foton m-2 s-1 şiddetinde mavi ışıkla yer değiştirilen yeşil ışığın, mikroalg büyümesinde ve biokütle artışında kontrol grubu olarak kullanılan kırmızı:mavi (1:2) grubuna göre istatistiksel olarak kayda değer bir artış sağladığı bulunmuştur. Yeşil ışık katkısının uygulandığı tüm deneysel gruplarda kontrol grubuna oranla klorofil konsantrasyonunda kayda değer bir artış gözlemlenmiştir. Bu çalışmada 3 μmol foton m-2 s-1 şiddetindeki yeşil ışık katkısının kırımızı:mavi ışık karışımı ile birlikte mikroalg büyümesinde, klorofil-a konsantrasyonunda ve kuru ağılık eldesinde kayda değer bir artışa neden olduğu bulunmuştur.

Keywords

• Chlamydomonas reinhardtii
• Yeşil Işık Katkısı
• Kırmızı Işık
• Mavi Işık
• Büyüme
• LED

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