Physiological and Biochemical Effects of Thermo-Priming on Wheat (Triticum aestivum L.) under Drought and Heat Stresses

Müge Teker Yıldız; Eda Günay; Okan Acar

doi:10.29130/dubited.1213671

TR EN

Kuraklık ve Isı Stresi altındaki Buğdayda Termo- Priming’in Fizyolojik ve Biyokimyasal Etkileri

Abstract

Tohum priming, bitkilerin stresli çevre koşullarına karşı stres toleransını arttırmaya yönelik fiziksel bir yöntemdir. Kuraklık ve yüksek sıcaklıklar, buğdayın büyümesini ve tane verimini sınırlayan önemli çevresel faktörlerdir. Çalışmamızın amacı kuraklık stresi (D) ve sıcaklık stresi (H) altındaki buğday tohumlarında termo-priming ile meydana gelen fizyolojik (çimlenme oranı, kök ve gövde uzunluğu, spesifik yaprak alanı (SLA), bağıl su içeriği (RWC), biyokütle, toplam klorofil miktarı (SPAD)) ve biyokimyasal (protein miktarı, hidrojen peroksit (H2O2) miktarı, katalaz aktivitesi (CAT), askorbat peroksidaz aktivitesi (APX), glutatyon redüktaz aktivitesi (GR)) değişimleri belirlemektir. Sonuçlarımız, gövde uzunluklarının kök uzunluklarına kıyasla D, H ve HD ile çarpıcı şekilde azaldığını gösterdi. Ayrıca kombine stres, diğer stres uygulamalarına kıyasla 60 dakikalık termo-priming ile RWC %6,8 oranında korunmuştur. Klorofil içeriği, D ve H ile önemli ölçüde azalırken, termo-priming bu düşüşle sınırlı kalmadı. Ayrıca SLA tüm stres tedavilerinde azalırken sadece 60 dk termo-priming (HDT60) ile %12 iyileştirdi. H2O2 kuraklık stresi ile artarken, tüm ısı stresi uygulamaları ile azalmıştır. Bunlardan HDT60'ın diğerlerinden daha etkili olduğu tespit edildi. GR aktiviteleri termo-priming ile yaklaşık %14-18, D ve H uygulamaları ile %5 arttırıldı. Ek olarak, HD uygulamasında 30 dakikalık termo-priming (HDT30) ile GR aktivitesi %5,8 artarken, yalnızca HD ile %3,2 arttı. Sonuç olarak, HDT60'ın buğday fidelerinde kuraklık ve ısı streslerine karşı biyokimyasal parametreler üzerinde etkili olduğu görülmüştür.

Keywords

Kuraklık stresi, Isı stresi, Osmotik stres, Termo-priming, Buğday

Physiological and Biochemical Effects of Thermo-Priming on Wheat (Triticum aestivum L.) under Drought and Heat Stresses

Abstract

Seed priming is a physical method for increasing the stress tolerance of crops against stressful environmental conditions. Drought and high temperatures are important environmental factors that limit the growth and grain yield of wheat. The aim of our study is to determine the physiological (germination rate, root and shoot length, specific leaf area (SLA), relative water content (RWC), biomass, total chlorophyll amount (SPAD)), and biochemical (protein amount, hydrogen peroxide (H2O2) amount, catalase activity (CAT), ascorbate peroxidase activity (APX), glutathione reductase activity (GR)) changes that occur with thermo-priming in wheat seeds under drought stress (D) and heat stress (H). Our results showed that shoot lengths were drastically reduced with D, H, and HD compared to root lengths. Besides, combined stress protected RWC by 6.8% with 60 min thermo-priming compared to other stress treatments. Chlorophyll content decreased dramatically with D and H, while thermo-priming wasn’t limited to that decrease. In addition, SLA was decreased with all stress treatments, while it healed only with 60 min thermo-priming (HDT60) by 12%. H2O2 was increased with drought stress, while reduced with all heat stress treatments. Among them, HDT60 was found to be more effective than the others. GR activities were increased with thermo-priming by 14-18%, with D and H by 5%. Additionally, GR activity was increased with 30 min thermo-priming (HDT30) in HD treatment by 5.8%, while only with HD by 3.2%. Consequently, HDT60 seemed to effectively on biochemical parameters in wheat seedlings against drought and heat stresses.

Keywords

Drought stress, Heat stress, Osmotic stress, Thermo-priming, Wheat

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Details

Primary Language

English

Subjects

Engineering

Journal Section

Research Article

Authors

Müge Teker Yıldız
0000-0001-7657-9811
Türkiye

Eda Günay
0000-0002-6616-6228
Türkiye

Okan Acar ^*
0000-0002-9818-8827
Türkiye

Publication Date

January 26, 2024

Submission Date

December 2, 2022

Acceptance Date

April 24, 2023

Published in Issue

Year 2024 Volume: 12 Number: 1

DOI

https://doi.org/10.29130/dubited.1213671

IZ

https://izlik.org/JA83EE37BD

APA

Teker Yıldız, M., Günay, E., & Acar, O. (2024). Physiological and Biochemical Effects of Thermo-Priming on Wheat (Triticum aestivum L.) under Drought and Heat Stresses. Duzce University Journal of Science and Technology, 12(1), 375-389. https://doi.org/10.29130/dubited.1213671

AMA

1.Teker Yıldız M, Günay E, Acar O. Physiological and Biochemical Effects of Thermo-Priming on Wheat (Triticum aestivum L.) under Drought and Heat Stresses. DUBİTED. 2024;12(1):375-389. doi:10.29130/dubited.1213671

Chicago

Teker Yıldız, Müge, Eda Günay, and Okan Acar. 2024. “Physiological and Biochemical Effects of Thermo-Priming on Wheat (Triticum Aestivum L.) under Drought and Heat Stresses”. Duzce University Journal of Science and Technology 12 (1): 375-89. https://doi.org/10.29130/dubited.1213671.

EndNote

Teker Yıldız M, Günay E, Acar O (January 1, 2024) Physiological and Biochemical Effects of Thermo-Priming on Wheat (Triticum aestivum L.) under Drought and Heat Stresses. Duzce University Journal of Science and Technology 12 1 375–389.

IEEE

[1]M. Teker Yıldız, E. Günay, and O. Acar, “Physiological and Biochemical Effects of Thermo-Priming on Wheat (Triticum aestivum L.) under Drought and Heat Stresses”, DUBİTED, vol. 12, no. 1, pp. 375–389, Jan. 2024, doi: 10.29130/dubited.1213671.

ISNAD

Teker Yıldız, Müge - Günay, Eda - Acar, Okan. “Physiological and Biochemical Effects of Thermo-Priming on Wheat (Triticum Aestivum L.) under Drought and Heat Stresses”. Duzce University Journal of Science and Technology 12/1 (January 1, 2024): 375-389. https://doi.org/10.29130/dubited.1213671.

JAMA

1.Teker Yıldız M, Günay E, Acar O. Physiological and Biochemical Effects of Thermo-Priming on Wheat (Triticum aestivum L.) under Drought and Heat Stresses. DUBİTED. 2024;12:375–389.

MLA

Teker Yıldız, Müge, et al. “Physiological and Biochemical Effects of Thermo-Priming on Wheat (Triticum Aestivum L.) under Drought and Heat Stresses”. Duzce University Journal of Science and Technology, vol. 12, no. 1, Jan. 2024, pp. 375-89, doi:10.29130/dubited.1213671.

Vancouver

1.Müge Teker Yıldız, Eda Günay, Okan Acar. Physiological and Biochemical Effects of Thermo-Priming on Wheat (Triticum aestivum L.) under Drought and Heat Stresses. DUBİTED. 2024 Jan. 1;12(1):375-89. doi:10.29130/dubited.1213671

Cited By

Effect of seed priming with salicylic acid on germination and biochemical traits of bread wheat (Triticum aestivum) cultivar Sain under salinity stress

Journal of Seed Research

https://doi.org/10.61882/yujs.11.2.111