Detection of Viruses in Oilseed Rape (Canola) in Samsun Province

Abstract

Oilseed rape, a member of the Brassicaceae, is one of the most important oil plants. Bafra Plain is one of the most important oilseed rape producing areas in Turkey. Surveys were done to determine the distribution of viruses in oilseed rape cultivated areas in Samsun province in 2021 and 2022. According to the results of field surveys, characteristic virus symptoms were observed on some oilseed rape plants. Leaf samples was collected from oilseed rape fields in Bafra Plain- Samsun province and tested for the presence of Cauliflower mosaic virus (CaMV), Turnip mosaic virus (TuMV), Cucumber mosaic virus (CMV), and Tomato spotted wilt virus (TSWV) by DAS-ELISA using commercial polyclonal antiserum. Result of serological tests showed that 8.06% of these samples were infected with CaMV (4.83%), and TSWV (3.22%). However, CMV and TuMV were not determined in canola samples.

Keywords

• Brassicaceae
• kanola
• virüs
• CaMV
• TSWV

Samsun İli Kanola Üretim Alanlarda Enfeksiyon Oluşturan Virüslerin Belirlenmesi

Abstract

Brassicaceae familyasına ait bir tür olan kanola (Brassica napus), önemli bir yağ bitkisidir. Bafra Ovası ülkemizin önemli kanola üretim alanlarından birisi konumundadır. Samsun ilinde kanola üretim alanlarında virüslerin yaygınlık durumunu belirlemek amacıyla 2021-2022 yılında sürveyler yapılmıştır. Arazide gözlemler sırasında bazı kanola bitkilerinde karakteristik virüs simptomları gözlenmiştir. Yapılan sürveyler sırasında, kanola üretim alanlarından yaprak örnekleri toplanmış ve bu örnekler virüs-spesifik ticari poliklonal antiserumlar kullanılarak Double antibody-Sandwich enzyme-linked immunosorbent assay (DAS-ELISA) yöntemi ile Cauliflower mosaic virus (CaMV), Turnip mosaic virus (TuMV), Cucumber mosaic virus (CMV), Tomato spotted wilt virus (TSWV) için analiz edilmiştir. Serolojik analizler sonucunda kanola örneklerinin %4.83’ünün CaMV, %3.22’sinin TSWV olmak üzere virüsle enfekteli (%8.06) olduğu belirlenirken, analiz edilen kanola örneklerinde CMV ve TuMV enfeksiyonuna rastlanmamıştır.

Keywords

• Brassicaceae
• kanola
• virüs
• CaMV
• TSWV

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