rFVIII Rekombinant Protein Üretiminde Sıvı Akışı, Karıştırma, Isı ve Kütle Transferi: Yukarı Akış ve Aşağı Akış Süreçleri

Year 2024, , 1358 - 1370, 31.07.2024

Tevfik Gemci

https://doi.org/10.29130/dubited.1300873

Abstract

Ana kan pıhtılaşma yolları, büyük ölçüde bir glikoprotein kofaktörü olan faktör VIII'e bağlıdır. X'e bağlı baskın bir hastalık olan Hemofili A, ticari olarak temin edilebilen çok karmaşık bir terapötik protein olan FVIII ile tedavi edilir. Şu anda en kapsamlı ve önemli pıhtılaşma faktörlerinden biridir. Hemofili A hastalarında hemorajik hastalığı tedavi etmek için iki izole edilmiş plazma ve rekombinant liyofilize FVIII konsantresi kullanılır. İnsan kanından ayrıştırılan plazmadan elde edilmiş ürünler hem insansı hem de hayvansal protein içermeyen ve ökaryotik hücrelerde kopyalanan rekombinant FVIII (rFVIII) ürünler ile ikame edilebilir. Sıvı akışı, karıştırma, ısı ve kütle transferi olayları ile ilgili olarak, rFVIII'in yukarı akış ve aşağı akış üretim işlemleri bu makalede kısaca gözden geçirilecektir.

Keywords

Rekombinant protein rFVIII üretimi, Yukarı akış ve aşağı akış süreçleri, Biyoreaktörlerde hücre çoğalması, Sıvı akışı, Isı ve kütle transferi

Project Number

Not applicable

Upstream and Downstream Processes of rFVIII Recombinant Protein Manufacturing in Respect to Fluid Flow, Mixing, Heat and Mass Transfer

Abstract

The intrinsic blood coagulation pathways depend heavily on factor VIII, a glycoprotein cofactor. Hemophilia A, an X-linked dominant disease, is treated with FVIII, a very complex therapeutic protein commercially available. It is currently one of the most extensive and significant coagulation factors. Two isolated plasma and recombinant lyophilized FVIII concentrates are used to treat hemorrhagic illness in hemophilia A sufferers. Plasma-extracted products separated from humanoid blood can be substituted with recombinant FVIII (rFVIII) products, which are free of both humanoid and carnal proteins and transcribed in eukaryotic cells. In regard to fluid flow, mixing, heat, and mass transfer phenomena, the upstream and downstream manufacturing processes of rFVIII will be briefly reviewed in this publication.

Keywords

Recombinant protein rFVIII manufacturing, Upstream and downstream processes, Cell growth in bioreactors, Fluid flow, Heat and mass transfer

Supporting Institution

No supporting institution

Project Number

Not applicable

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