Molecularly Imprinted Chitosan Modified Quartz Tuning Fork Sensors for Real Time Biosensing in Liquid Environment

Abstract

Several new sensing technologies have emerged to meet the escalating demand for accurate and rapid diagnosis. We present an overview of the development of highly sensitive and selective Quartz Tuning Fork (QTF)-based sensors in a liquid environment, which will be critically important for contemporary diagnostic methods reliant on sensing technologies. The purpose of this study is to modify QTF prongs using molecularly imprinted chitosan, in combination with the operation of a quartz tuning fork as a piezoelectric crystal for biomedical applications. Through real-time data acquisition, we evaluate QTF resonance frequency shifts in dry and liquid environments using a model protein, BSA. As a result, the QTF-based sensor fails to detect BSA in dry conditions. It is however possible to measure frequency shifts ranging from 5 to 25 µg /mL within a liquid matrix. There is a rapid equilibration response time of 2 to 10 minutes depending on the concentration of BSA in the sensor. With the developed QTF-based sensor, a sensitivity of 1.1069 Hz/ µg has been achieved within the liquid matrix. As a result of the excellent properties of molecularly imprinted chitosan, it has been possible to develop a QTF-based biosensor capable of acquiring real-time data even when it is in liquid solutions.

Keywords

• Quartz tuning fork
• Mass sensitive sensor
• Molecularly imprinted polymer
• chitosan
• bovine serum albumin

Sıvı Ortamda Gerçek Zamanlı Biyoalgılama için Moleküler Baskılı Kitosan Modifiye Kuvars Ayar Çatalı Sensör Geliştirilmesi

Abstract

Yüksek hassasiyet ve seçicilikle çalışan, doğru ve hızlı teşhis talebini karşılamak üzere bir dizi yeni duyusal teknoloji ortaya çıkmıştır. Bu çalışma kapsamında, sensör teknolojilerine dayalı çağdaş teşhis yöntemleri için kritik önem taşıyan sıvı ortamda çalışan Kuvars Ayar Çatal (QTF) tabanlı sensörler tasarımı üzerine detaylı bilgi sunmaktadır. Bu çalışma, biyomedikal uygulamalar için bir çevirici olarak kuvars ayar çatalın piezoelektrik kristal olarak kullanılması ile, QTF çatallarını moleküler baskılı kitosan ile modifiye etmeyi amaçlamaktadır Gerçek zamanlı veri toplama ile, model bir protein olan BSA kullanarak QTF rezonans frekansındaki değişimleri hem kuru hem de sıvı ortamlarda takip edilmiştir. Elde edilen sonuçlara göre, QTF tabanlı sensör, kuru koşullarda BSA tespiti yapamamaktadır. Ancak sıvı matris içinde 5 ila 25 µg/mL aralığında frekans değişimleri ölçülebildiği gösterilmiştir. Sensördeki BSA konsantrasyonuna bağlı olarak, tepki süresi BSA konsantrasyonuna bağlı olarak 2 ila 10 dakika arasında değişmektedir. Geliştirilen QTF tabanlı sensörle, sıvı matris içinde 1.1069 Hz/ µg hassasiyette ölçüm yapabilmektedir. Moleküler baskılı kitosanın üstün özellikleri sayesinde, sıvı çözeltilerde bile gerçek zamanlı veri toplama yeteneğine sahip bir QTF tabanlı biyosensör geliştirilmiştir.

Keywords

• Kuvars ayar çatalı
• Kütle hassas sensör
• Moleküler baskılı polimer
• Kitosan
• Sığır serum albumin

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